This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ukimura, A. Articles by Hirai, K. Search for Related Content PubMed PubMed Citation Articles by Ukimura, A. Articles by Hirai, K.

American Journal of Pathology, Vol 150, 2061-2074, Copyright © 1997 by American Society for Investigative Pathology

REGULAR ARTICLES

Intracellular viral localization in murine coxsackievirus-B3 myocarditis. Ultrastructural study by electron microscopic in situ hybridization

A Ukimura, H Deguchi, Y Kitaura, S Fujioka, M Hirasawa, K Kawamura and K Hirai
Department of Internal Medicine, Osaka Medical College, Japan.

Group B Coxsackieviruses are a common cause of myocarditis. To detect the viral genome and its localization in the myocardium, we examined C3H/He mice with Coxsackievirus B3 (CVB3) myocarditis on days 5, 8, and 14 after inoculation by the reverse transcriptase polymerase chain reaction and by in situ hybridization. Sense and antisense CVB3 RNA were detected in the myocardium of all mice up to day 14 by reverse transcriptase polymerase chain reaction. Light microscopic in situ hybridization with a cDNA probe for CVB3 showed clusters of positive signals in the areas of myocardial necrosis and cell infiltration. With electron microscopic in situ hybridization, CVB3 RNA was detected in the cytoplasm of cardiocytes, between the myofibrils, near the mitochondria, and in tubular or vesicular structures. Viral RNA was also detected in necrotic debris, in the cytoplasm of macrophages, and in the cytoplasm of interstitial fibroblasts. These findings suggest that CVB3 RNA is replicated in the cytoplasm of cardiocytes, transferred into tubular or vesicular structures, released into the interstitium, and phagocytosed by macrophages. Some positive signals were also detected in the cytoplasm of cardiocytes showing close contact with infiltrating lymphocytes, suggesting that the lymphocytes recognized virus-infected cardiocytes and caused cell-mediated immune cardiocyte damage.

This article has been cited by other articles:

				S.-M. Kim, J.-H. Park, S.-K. Chung, J.-Y. Kim, H.-Y. Hwang, K.-C. Chung, I. Jo, S.-I. Park, and J.-H. Nam Coxsackievirus B3 Infection Induces cyr61 Activation via JNK To Mediate Cell Death J. Virol., December 15, 2004; 78(24): 13479 - 13488. [Abstract] [Full Text] [PDF]

				E Arbustini, E Porcu, O Bellini, M Grasso, A Pilotto, B Dal Bello, P Morbini, M Diegoli, A Gavazzi, G Specchia, et al. Enteroviral infection causing fatal myocarditis and subclinical myopathy Heart, January 1, 2000; 83(1): 86 - 90. [Abstract] [Full Text] [PDF]

				D. Yang, J. Yu, Z. Luo, C. M. Carthy, J. E. Wilson, Z. Liu, and B. M. McManus Viral Myocarditis : Identification of Five Differentially Expressed Genes in Coxsackievirus B3–Infected Mouse Heart Circ. Res., April 2, 1999; 84(6): 704 - 712. [Abstract] [Full Text] [PDF]