This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wacker, H. H. Articles by Parwaresch, R. Search for Related Content PubMed PubMed Citation Articles by Wacker, H. H. Articles by Parwaresch, R.

American Journal of Pathology, Vol 151, 423-434, Copyright © 1997 by American Society for Investigative Pathology

REGULAR ARTICLES

Sinus-lining cells of the lymph nodes recognized as a dendritic cell type by the new monoclonal antibody Ki-M9

HH Wacker, SO Frahm, HJ Heidebrecht and R Parwaresch
Institute of Pathology, German Association of Pathologists, University Kiel, Germany.

In the immunobiological characterization of lymph node cells, sinus- lining cells (SLCs) have been given little attention mainly due to the difficulties in their recognition. Ki-M9 is a new monoclonal antibody (MAb) selected for its unique capability to visualize SLCs in human lymph nodes. The details were established by light and electron microscopy and immunoprecipitation of the corresponding biosynthetically labeled antigen. Ki-M9 recognizes a 70-kd protein localized on the surface membrane of SLCs. In the lymphoid tissue, a mild reactivity was exclusively encountered on follicular dendritic reticulum cells in the germinal centers of secondary lymphoid follicles. In other organs, some squamous epithelial and myoepithelial cells were recognized by this antibody. Immunomonitoring of SLCs on light and electron microscopic levels revealed their dendritic morphology, lack of phagosomes, and their close association with type IV collagen fibers. Considering the occurrence of typical dendritic SLCs on the front line of antigen flood, we propose that SLCs be investigated for a possible antigen-binding property.

This article has been cited by other articles:

				D. Lissandrini, W. Vermi, M. Vezzalini, S. Sozzani, F. Facchetti, G. Bellone, A. Mafficini, F. Gentili, M. G. Ennas, C. Tecchio, et al. Receptor-type protein tyrosine phosphatase gamma (PTP{gamma}), a new identifier for myeloid dendritic cells and specialized macrophages Blood, December 15, 2006; 108(13): 4223 - 4231. [Abstract] [Full Text] [PDF]

				H Hattori Caution should be taken in using CD31 for distinguishing the vasculature of lymph nodes J. Clin. Pathol., August 1, 2003; 56(8): 638 - 639. [Full Text]

				J. S. Ahn, A. Konno, J. A. Gebe, A. Aruffo, M. J. Hamilton, Y. H. Park, and W. C. Davis Scavenger receptor cysteine-rich domains 9 and 11 of WC1 are receptors for the WC1 counter receptor J. Leukoc. Biol., August 1, 2002; 72(2): 382 - 390. [Abstract] [Full Text] [PDF]

				E. P. Kaldjian, J. E. Gretz, A. O. Anderson, Y. Shi, and S. Shaw Spatial and molecular organization of lymph node T cell cortex: a labyrinthine cavity bounded by an epithelium-like monolayer of fibroblastic reticular cells anchored to basement membrane-like extracellular matrix Int. Immunol., October 1, 2001; 13(10): 1243 - 1253. [Abstract] [Full Text] [PDF]