| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 151, 1141-1152, Copyright © 1997 by American Society for Investigative Pathology
REGULAR ARTICLES |
S Breiteneder-Geleff, K Matsui, A Soleiman, P Meraner, H Poczewski, R Kalt, G Schaffner and D Kerjaschki
Department of Clinical Pathology, University of Vienna, Austria.
Puromycin aminonucleoside nephrosis (PAN), a rat model of human minimal change nephropathy, is characterized by extensive flattening of glomerular epithelial cell (podocyte) foot processes and by severe proteinuria. For comparison of expression of glomerular membrane proteins of normal and PAN rats, a membrane protein fraction of isolated rat glomeruli was prepared and monoclonal antibodies were raised against it. An IgG-secreting clone designated LF3 was selected that specifically immunolabeled podocytes of normal but not of PAN rats. The antigen of LF3 IgG was identified as a 43-kd glycoprotein. Molecular cloning of its cDNA was performed in a delta gt11 expression library prepared from mRNA of isolated rat glomeruli. The predicted amino acid sequence indicated a 166-amino-acid integral membrane protein with a single membrane-spanning domain, two potential phosphorylation sites in its short cytoplasmic tail, and six potential O-glycosylation sites in the large ectodomain. High amino acid sequence identities were found to membrane glycoproteins of rat lung and bone and mouse thymus epithelial cells as well as to a phorbol-ester-induced protein in a mouse osteoblast cell line and to a canine influenza C virus receptor. In PAN, expression of this 43-kd protein was selectively reduced to < 30%, as determined by quantitative immunogold electron microscopy, immunoblotting, and Northern blotting. These data provide evidence that transcription of the 43-kd transmembrane podocyte glycoprotein is specifically down-regulated in PAN. To indicate that this protein could be associated with transformation of arborized foot processes to flat feet (Latin, pes planus) we have called it podoplanin.
This article has been cited by other articles:
 |
|
 |
|
  K. Koop, M. Eikmans, M. Wehland, H. Baelde, D. Ijpelaar, R. Kreutz, H. Kawachi, D. Kerjaschki, E. de Heer, and J. A. Bruijn Selective Loss of Podoplanin Protein Expression Accompanies Proteinuria and Precedes Alterations in Podocyte Morphology in a Spontaneous Proteinuric Rat Model Am. J. Pathol., August 1, 2008; 173(2): 315 - 326. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. H. T. IJpelaar, A. Schulz, K. Koop, M. Schlesener, J. A. Bruijn, D. Kerjaschki, R. Kreutz, and E. de Heer Glomerular hypertrophy precedes albuminuria and segmental loss of podoplanin in podocytes in Munich-Wistar-Fromter rats Am J Physiol Renal Physiol, April 1, 2008; 294(4): F758 - F767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Suzuki-Inoue, Y. Kato, O. Inoue, M. K. Kaneko, K. Mishima, Y. Yatomi, Y. Yamazaki, H. Narimatsu, and Y. Ozaki Involvement of the Snake Toxin Receptor CLEC-2, in Podoplanin-mediated Platelet Activation, by Cancer Cells J. Biol. Chem., September 7, 2007; 282(36): 25993 - 26001. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jackowski, M. Janusch, E. Fiedler, W. C. Marsch, E. J. Ulbrich, G. Gaisbauer, J. Dunst, D. Kerjaschki, and P. Helmbold Radiogenic Lymphangiogenesis in the Skin Am. J. Pathol., July 1, 2007; 171(1): 338 - 348. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Hinojar-Gutierrez, M.-E. Fernandez-Contreras, R. Gonzalez-Gonzalez, M.-J. Fernandez-Luque, A. Hinojar-Arzadun, M. Quintanilla, and C. Gamallo Intratumoral Lymphatic Vessels and VEGF-C Expression Are Predictive Factors of Lymph Node Relapse in T1-T4 N0 Laryngopharyngeal Squamous Cell Carcinoma Ann. Surg. Oncol., January 1, 2007; 14(1): 248 - 257. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Martin-Villar, D. Megias, S. Castel, M. M. Yurrita, S. Vilaro, and M. Quintanilla Podoplanin binds ERM proteins to activate RhoA and promote epithelial-mesenchymal transition J. Cell Sci., November 1, 2006; 119(21): 4541 - 4553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Kreuger, I. Nilsson, D. Kerjaschki, T. Petrova, K. Alitalo, and L. Claesson-Welsh Early Lymph Vessel Development From Embryonic Stem Cells Arterioscler. Thromb. Vasc. Biol., May 1, 2006; 26(5): 1073 - 1078. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y Nakamura, H Yasuoka, M Tsujimoto, K Kurozumi, M Nakahara, K Nakao, and K Kakudo Importance of lymph vessels in gastric cancer: a prognostic indicator in general and a predictor for lymph node metastasis in early stage cancer J. Clin. Pathol., January 1, 2006; 59(1): 77 - 82. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Schacht, S. S. Dadras, L. A. Johnson, D. G. Jackson, Y.-K. Hong, and M. Detmar Up-Regulation of the Lymphatic Marker Podoplanin, a Mucin-Type Transmembrane Glycoprotein, in Human Squamous Cell Carcinomas and Germ Cell Tumors Am. J. Pathol., March 1, 2005; 166(3): 913 - 921. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Chen, K. Takenaka, E. Ogawa, K. Yanagihara, Y. Otake, H. Wada, and F. Tanaka Flt-4-Positive Endothelial Cell Density and Its Clinical Significance in Non-Small Cell Lung Cancer Clin. Cancer Res., December 15, 2004; 10(24): 8548 - 8553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.C. McElroy and M. Kasper The use of alveolar epithelial type I cell-selective markers to investigate lung injury and repair Eur. Respir. J., October 1, 2004; 24(4): 664 - 673. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kaneko, Y. Kato, A. Kunita, N. Fujita, T. Tsuruo, and M. Osawa Functional Sialylated O-Glycan to Platelet Aggregation on Aggrus (T1{alpha}/Podoplanin) Molecules Expressed in Chinese Hamster Ovary Cells J. Biol. Chem., September 10, 2004; 279(37): 38838 - 38843. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Colvin Emphatically Lymphatic J. Am. Soc. Nephrol., March 1, 2004; 15(3): 827 - 829. [Full Text] [PDF]
|
|
|
|
 |
|
 M. Sharma, R. Sharma, E. T. McCarthy, and V. J. Savin The Focal Segmental Glomerulosclerosis Permeability Factor: Biochemical Characteristics and Biological Effects Experimental Biology and Medicine, January 1, 2004; 229(1): 85 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kato, N. Fujita, A. Kunita, S. Sato, M. Kaneko, M. Osawa, and T. Tsuruo Molecular Identification of Aggrus/T1{alpha} as a Platelet Aggregation-inducing Factor Expressed in Colorectal Tumors J. Biol. Chem., December 19, 2003; 278(51): 51599 - 51605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Schmid, A. Henger, C. D. Cohen, K. Frach, H.-J. Grone, D. Schlondorff, and M. Kretzler Gene Expression Profiles of Podocyte-Associated Molecules as Diagnostic Markers in Acquired Proteinuric Diseases J. Am. Soc. Nephrol., November 1, 2003; 14(11): 2958 - 2966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Suzuki, T. Ito, E. Imai, M. Yamato, H. Iwatani, H. Kawachi, and M. Hori Retinoids Regulate the Repairing Process of the Podocytes in Puromycin Aminonucleoside-induced Nephrotic Rats J. Am. Soc. Nephrol., April 1, 2003; 14(4): 981 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Pavenstadt, W. Kriz, and M. Kretzler Cell Biology of the Glomerular Podocyte Physiol Rev, January 1, 2003; 83(1): 253 - 307. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Cursiefen, U. Schlotzer-Schrehardt, M. Kuchle, L. Sorokin, S. Breiteneder-Geleff, K. Alitalo, and D. Jackson Lymphatic Vessels in Vascularized Human Corneas: Immunohistochemical Investigation Using LYVE-1 and Podoplanin Invest. Ophthalmol. Vis. Sci., July 1, 2002; 43(7): 2127 - 2135. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. STACKER, M. E. BALDWIN, and M. G. ACHEN The role of tumor lymphangiogenesis in metastatic spread FASEB J, July 1, 2002; 16(9): 922 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Jussila and K. Alitalo Vascular Growth Factors and Lymphangiogenesis Physiol Rev, July 1, 2002; 82(3): 673 - 700. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Palmen, S. Lehtonen, A. Ora, D. Kerjaschki, C. Antignac, E. Lehtonen, and H. Holthofer Interaction of Endogenous Nephrin and CD2-Associated Protein in Mouse Epithelial M-1 Cell Line J. Am. Soc. Nephrol., July 1, 2002; 13(7): 1766 - 1772. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Boucherot, R. Schreiber, H. Pavenstadt, and K. Kunzelmann Cloning and expression of the mouse glomerular podoplanin homologue gp38P Nephrol. Dial. Transplant., June 1, 2002; 17(6): 978 - 984. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Kriehuber, S. Breiteneder-Geleff, M. Groeger, A. Soleiman, S. F. Schoppmann, G. Stingl, D. Kerjaschki, and D. Maurer Isolation and Characterization of Dermal Lymphatic and Blood Endothelial Cells Reveal Stable and Functionally Specialized Cell Lineages J. Exp. Med., September 17, 2001; 194(6): 797 - 808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Birner, A. Obermair, M. Schindl, H. Kowalski, G. Breitenecker, and G. Oberhuber Selective Immunohistochemical Staining of Blood and Lymphatic Vessels Reveals Independent Prognostic Influence of Blood and Lymphatic Vessel Invasion in Early-Stage Cervical Cancer Clin. Cancer Res., January 1, 2001; 7(1): 93 - 97. [Abstract] [Full Text]
|
|
|
|
|
|
T. A. PARTANEN, J. AROLA, A. SAARISTO, L. JUSSILA, A. ORA, M. MIETTINEN, S. A. STACKER, M. G. ACHEN, and K. ALITALO VEGF-C and VEGF-D expression in neuroendocrine cells and their receptor, VEGFR-3, in fenestrated blood vessels in human tissues FASEB J, October 1, 2000; 14(13): 2087 - 2096. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Takeda, W. Y. Go, R. A. Orlando, and M. G. Farquhar Expression of Podocalyxin Inhibits Cell-Cell Adhesion and Modifies Junctional Properties in Madin-Darby Canine Kidney Cells Mol. Biol. Cell, September 1, 2000; 11(9): 3219 - 3232. [Abstract] [Full Text]
|
|
|
|
|
|
C. J. I. Raats, J. van den Born, M. A. H. Bakker, B. Oppers-Walgreen, B. J. M. Pisa, H. B. P. M. Dijkman, K. J. M. Assmann, and J. H. M. Berden Expression of Agrin, Dystroglycan, and Utrophin in Normal Renal Tissue and in Experimental Glomerulopathies Am. J. Pathol., May 1, 2000; 156(5): 1749 - 1765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Pavenstadt Roles of the podocyte in glomerular function Am J Physiol Renal Physiol, February 1, 2000; 278(2): F173 - F179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Binder, H. Weiher, M. Exner, and D. Kerjaschki Glomerular Overproduction of Oxygen Radicals in Mpv17 Gene-Inactivated Mice Causes Podocyte Foot Process Flattening and Proteinuria : A Model of Steroid-Resistant Nephrosis Sensitive to RadicalScavenger Therapy Am. J. Pathol., April 1, 1999; 154(4): 1067 - 1075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Breiteneder-Geleff, A. Soleiman, H. Kowalski, R. Horvat, G. Amann, E. Kriehuber, K. Diem, W. Weninger, E. Tschachler, K. Alitalo, et al. Angiosarcomas Express Mixed Endothelial Phenotypes of Blood and Lymphatic Capillaries : Podoplanin as a Specific Marker for Lymphatic Endothelium Am. J. Pathol., February 1, 1999; 154(2): 385 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Scholl, C Gamallo, S Vilar?o, and M Quintanilla Identification of PA2.26 antigen as a novel cell-surface mucin-type glycoprotein that induces plasma membrane extensions and increased motility in keratinocytes J. Cell Sci., January 12, 1999; 112(24): 4601 - 4613. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
