| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 152, 23-27, Copyright © 1998 by American Society for Investigative Pathology
REGULAR ARTICLES |
JA Friedline, SH Garrett, S Somji, JH Todd and DA Sens
Robert C. Byrd Health Sciences Center, Department of Pathology, West Virginia University, Morgantown 26506-9203, USA.
The goal of this study was to determine which of the 10 functional metallothionein (MT) genes are expressed in four human breast cancer cell lines and whether expression varies among the cell lines. Using reverse transcription polymerase chain reaction (RT-PCR) technology, it was shown that there was no expression of mRNA for the MT-1A, MT-1B, MT- 1F, MT-1G, MT-1H, MT-3, and MT-4 genes in any of the four cell lines. All four cell lines were shown to express mRNA for the MT-2A and MT-1X genes. The expression level of mRNA for the MT-2A gene demonstrated modest differences among the cell lines, whereas expression of the MT- 1X gene was consistent. In contrast, mRNA for the MT-1E gene was expressed in only two of the four cell lines and expression correlated to the estrogen receptor status of the cell lines. The two estrogen- receptor-positive cell lines showed no mRNA expression for the MT-1E gene. In the two estrogen-receptor-negative cell lines, mRNA expression for the MT-1E gene was elevated with expression levels similar to the housekeeping gene, glyceraldehyde-3-phosphate dehydrogenase. The cellular content of MT protein was also shown to be elevated in the estrogen-receptor-negative cell lines that express MT-1E mRNA. These results suggest a possible relationship between estrogen receptor status and MT-1E gene expression in human breast cancer.
This article has been cited by other articles:
 |
|
 |
|
  A. G. Scibetta, S. Santangelo, J. Coleman, D. Hall, T. Chaplin, J. Copier, S. Catchpole, J. Burchell, and J. Taylor-Papadimitriou Functional Analysis of the Transcription Repressor PLU-1/JARID1B Mol. Cell. Biol., October 15, 2007; 27(20): 7220 - 7235. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. D. Zhou, M. A. Sens, S. H. Garrett, S. Somji, S. Park, V. Gurel, and D. A. Sens Enhanced Expression of Metallothionein Isoform 3 Protein in Tumor Heterotransplants Derived from As+3- and Cd+2-Transformed Human Urothelial Cells Toxicol. Sci., October 1, 2006; 93(2): 322 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Gurel, D. A. Sens, S. Somji, S. H. Garrett, T. Weiland, and M. A. Sens Post-Transcriptional Regulation of Metallothionein Isoform 1 and 2 Expression in the Human Breast and the MCF-10A Cell Line Toxicol. Sci., June 1, 2005; 85(2): 906 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Lacroix, R-A Toillon, and G Leclercq Stable 'portrait' of breast tumors during progression: data from biology, pathology and genetics Endocr. Relat. Cancer, September 1, 2004; 11(3): 497 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ueda, S. Wang, N. Dumont, J. Y. Yi, Y. Koh, and C. L. Arteaga Overexpression of HER2 (erbB2) in Human Breast Epithelial Cells Unmasks Transforming Growth Factor {beta}-induced Cell Motility J. Biol. Chem., June 4, 2004; 279(23): 24505 - 24513. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-K. Tai, O. J.-K. Tan, V. T.-K. Chow, R. Jin, J. L. Jones, P.-H. Tan, A. Jayasurya, and B.-H. Bay Differential Expression of Metallothionein 1 and 2 Isoforms in Breast Cancer Lines with Different Invasive Potential: Identification of a Novel Nonsilent Metallothionein-1H Mutant Variant Am. J. Pathol., November 1, 2003; 163(5): 2009 - 2019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Liu, A. R. Bauskin, J. Zaunders, D. A. Brown, S. Pankurst, P. J. Russell, and S. N. Breit Macrophage Inhibitory Cytokine 1 Reduces Cell Adhesion and Induces Apoptosis in Prostate Cancer Cells Cancer Res., August 15, 2003; 63(16): 5034 - 5040. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
