This Article Order Full text via Infotrieve Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Takahashi, K. Articles by Takatsu, K. Search for Related Content PubMed PubMed Citation Articles by Takahashi, K. Articles by Takatsu, K.

American Journal of Pathology, Vol 152, 445-456, Copyright © 1998 by American Society for Investigative Pathology

REGULAR ARTICLES

Effects of granulocyte/macrophage colony-stimulating factor on the development and differentiation of CD5-positive macrophages and their potential derivation from a CD5-positive B-cell lineage in mice

K Takahashi, K Miyakawa, AA Wynn, KI Nakayama, YY Myint, M Naito, LD Shultz, A Tominaga and K Takatsu
Second Department of Pathology, Kumamoto University School of Medicine, Honjo, Japan.

In co-cultures of either the murine pre-B cell line J13, fetal liver cells, or adult peritoneal or bone marrow cells with ST2 mouse bone marrow stromal cells in the presence of granulocyte/macrophage colony- stimulating factor (GM-CSF), the development of CD5+ macrophages was demonstrated by immunohistochemical staining and flow cytometry. Although CD5+ macrophages were not present in the peritoneal cavities of normal mice, approximately 30% of the peritoneal macrophages in viable motheaten (mev/mev) mice, deficient in SHP-1 protein tyrosine phosphatase, expressed cell surface CD5 and B220, markers for B cells. In the mev/mev mice, GM-CSF level in peritoneal fluid was increased significantly. At 5 days after daily intravenous injection with GM-CSF, many CD5+ macrophages appeared in the peritoneal cavity and in omental milky spots of normal mice but fewer in osteopetrosis (op) mutant mice, deficient in macrophage (M)-CSF. These results indicate that GM-CSF, in combination with M-CSF, induces the development and differentiation of CD5+ macrophages in the peritoneal cavity, particularly in the omental milky spots of mice. In the peritoneal cavity of GM-CSF-treated mice, the percentages of hematopoietic progenitor cells doubly positive for CD5 and CD34 or c-kit and of macrophage precursor cells doubly positive for CD5 and ER-MP58 or ER-MP20 were increased significantly during the development of CD5+ macrophages and CD5 B cells, suggesting that CD5+ macrophages and B cells may share a bipotential progenitor in vivo.

This article has been cited by other articles:

				F. B. Aiello, J. R. Keller, K. D. Klarmann, G. Dranoff, R. Mazzucchelli, and S. K. Durum IL-7 Induces Myelopoiesis and Erythropoiesis J. Immunol., February 1, 2007; 178(3): 1553 - 1563. [Abstract] [Full Text] [PDF]

				A. D. Cook, E. L. Braine, and J. A. Hamilton Stimulus-Dependent Requirement for Granulocyte-Macrophage Colony-Stimulating Factor in Inflammation J. Immunol., October 1, 2004; 173(7): 4643 - 4651. [Abstract] [Full Text] [PDF]

				M. Y. Chiang and J. G. Monroe Role for Transcription Pax5A Factor in Maintaining Commitment to the B Cell Lineage by Selective Inhibition of Granulocyte-Macrophage Colony-Stimulating Factor Receptor Expression J. Immunol., May 15, 2001; 166(10): 6091 - 6098. [Abstract] [Full Text] [PDF]

				H.-C. Hsu, L. D. Shultz, X. Su, J. Shi, P.-A. Yang, M. J. Relyea, H.-G. Zhang, and J. D. Mountz Mutation of the Hematopoietic Cell Phosphatase (Hcph) Gene Is Associated with Resistance to {{gamma}}-Irradiation-Induced Apoptosis in Src Homology Protein Tyrosine Phosphatase (SHP)-1-Deficient ""Motheaten"" Mutant Mice J. Immunol., January 15, 2001; 166(2): 772 - 780. [Abstract] [Full Text] [PDF]

				A. A. Wynn, K. Miyakawa, E. Miyata, G. Dranoff, M. Takeya, and K. Takahashi Role of Granulocyte/Macrophage Colony-Stimulating Factor in Zymocel-Induced Hepatic Granuloma Formation Am. J. Pathol., January 1, 2001; 158(1): 131 - 145. [Abstract] [Full Text] [PDF]

				J. Jongstra-Bilen, V. L. Misener, C. Wang, H. Ginzberg, A. Auerbach, A. L. Joyner, G. P. Downey, and J. Jongstra LSP1 modulates leukocyte populations in resting and inflamed peritoneum Blood, September 1, 2000; 96(5): 1827 - 1835. [Abstract] [Full Text] [PDF]

				Y. Y. Myint, K. Miyakawa, M. Naito, L. D Shultz, Y. Oike, K.-i. Yamamura, and K. Takahashi Granulocyte/Macrophage Colony-Stimulating Factor and Interleukin-3 Correct Osteopetrosis in Mice with Osteopetrosis Mutation Am. J. Pathol., February 1, 1999; 154(2): 553 - 566. [Abstract] [Full Text] [PDF]