| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 152, 805-813, Copyright © 1998 by American Society for Investigative Pathology
REGULAR ARTICLES |
WG Degen, LC van Kempen, EG Gijzen, JJ van Groningen, Y van Kooyk, HP Bloemers and GW Swart
Department of Biochemistry, University of Nijmegen, The Netherlands.
From a differential mRNA display comparing a non- and a highly metastasizing human melanoma cell line, we isolated and characterized memD. memD is preferentially expressed in the highly metastasizing melanoma cell lines of a larger panel. The encoded protein, MEMD, is identical to activated leukocyte cell adhesion molecule (ALCAM), a recently identified ligand of CD6. ALCAM is involved in homophylic (ALCAM-ALCAM) and heterophylic (ALCAM-CD6) cell adhesion interactions. We have studied MEMD/ALCAM cell-cell interactions between human melanoma cells. The expression of this cell adhesion molecule not only correlates with enhanced metastatic properties and with aggregational behavior of human melanoma cells as tested by FACS analysis, but transfection experiments also make clear that MEMD/ALCAM expression is essential for cell-cell interaction of the investigated human melanoma cells. As the melanoma cell lines analyzed are all CD6 negative, these results strongly suggest that MEMD/ALCAM is an adhesion molecule mediating homophylic clustering of melanoma cells. MEMD/ALCAM expression is not restricted to subsets of leukocytes and melanoma cells, it can also be found in healthy organs and in several other malignant tumor cell lines. Besides, MEMD/ALCAM is also expressed in cultured endothelial cells, pericytes and melanocytes, in xenografts derived from the radial and vertical growth phase and in 4 of 13 melanoma metastasis lesions. The potential role is discussed of MEMD/ALCAM mediated cell-cell interactions in migration of mobile cells (ie, activated leukocytes, metastasizing tumor cells) through tissues.
This article has been cited by other articles:
 |
|
 |
|
  J. W.J. van Kilsdonk, R. H. Wilting, M. Bergers, G. N.P. van Muijen, J. Schalkwijk, L. C.L.T. van Kempen, and G. W.M. Swart Attenuation of Melanoma Invasion by a Secreted Variant of Activated Leukocyte Cell Adhesion Molecule Cancer Res., May 15, 2008; 68(10): 3671 - 3679. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Mezzanzanica, M. Fabbi, M. Bagnoli, S. Staurengo, M. Losa, E. Balladore, P. Alberti, L. Lusa, A. Ditto, S. Ferrini, et al. Subcellular Localization of Activated Leukocyte Cell Adhesion Molecule Is a Molecular Predictor of Survival in Ovarian Carcinoma Patients Clin. Cancer Res., March 15, 2008; 14(6): 1726 - 1733. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-C. Choi, K. D. Kim, J.-T. Kim, J. W. Kim, H. G. Lee, J.-M. Kim, Y.-S. Jang, D.-Y. Yoon, K. I. Kim, Y. Yang, et al. Expression of human NDRG2 by myeloid dendritic cells inhibits down-regulation of activated leukocyte cell adhesion molecule (ALCAM) and contributes to maintenance of T cell stimulatory activity J. Leukoc. Biol., January 1, 2008; 83(1): 89 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. te Riet, A. W. Zimmerman, A. Cambi, B. Joosten, S. Speller, R. Torensma, F. N. van Leeuwen, C. G. Figdor, and F. de Lange Distinct kinetic and mechanical properties govern ALCAM-mediated interactions as shown by single-molecule force spectroscopy J. Cell Sci., November 15, 2007; 120(22): 3965 - 3976. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Ruan, A. Sassoon, F. An, J. P. Simko, and B. Liu Identification of Clinically Significant Tumor Antigens by Selecting Phage Antibody Library on Tumor Cells in Situ Using Laser Capture Microdissection Mol. Cell. Proteomics, December 1, 2006; 5(12): 2364 - 2373. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Kato, Y. Tanaka, M. Hayashi, K. Okawa, and N. Minato Involvement of CD166 in the Activation of Human {gamma}{delta}T Cells by Tumor Cells Sensitized with Nonpeptide Antigens J. Immunol., July 15, 2006; 177(2): 877 - 884. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Burkhardt, E Mayordomo, K-J Winzer, F Fritzsche, T Gansukh, S Pahl, W Weichert, C Denkert, H Guski, M Dietel, et al. Cytoplasmic overexpression of ALCAM is prognostic of disease progression in breast cancer J. Clin. Pathol., April 1, 2006; 59(4): 403 - 409. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Lunter, J. W.J. van Kilsdonk, H. van Beek, I. M.H.A. Cornelissen, M. Bergers, P. H.G.M. Willems, G. N.P. van Muijen, and G. W.M. Swart Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166/MEMD), a Novel Actor in Invasive Growth, Controls Matrix Metalloproteinase Activity Cancer Res., October 1, 2005; 65(19): 8801 - 8808. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Piazza, E. Cha, I. Bongarzone, S. Canevari, A. Bolognesi, L. Polito, A. Bargellesi, F. Sassi, S. Ferrini, and M. Fabbi Internalization and recycling of ALCAM/CD166 detected by a fully human single-chain recombinant antibody J. Cell Sci., April 1, 2005; 118(7): 1515 - 1525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Ikeda and T. Quertermous Molecular Isolation and Characterization of a Soluble Isoform of Activated Leukocyte Cell Adhesion Molecule That Modulates Endothelial Cell Function J. Biol. Chem., December 31, 2004; 279(53): 55315 - 55323. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Saifullah, D. A. Fox, S. Sarkar, S. M. A. Abidi, J. Endres, J. Piktel, T. M. Haqqi, and N. G. Singer Expression and Characterization of a Novel CD6 Ligand in Cells Derived from Joint and Epithelial Tissues J. Immunol., November 15, 2004; 173(10): 6125 - 6133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W Weichert, T Knosel, J Bellach, M Dietel, and G Kristiansen ALCAM/CD166 is overexpressed in colorectal carcinoma and correlates with shortened patient survival J. Clin. Pathol., November 1, 2004; 57(11): 1160 - 1164. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Uhlenbrock, A. Eberth, U. Herbrand, N. Daryab, P. Stege, F. Meier, P. Friedl, J. G. Collard, and M. R. Ahmadian The RacGEF Tiam1 inhibits migration and invasion of metastatic melanoma via a novel adhesive mechanism J. Cell Sci., September 15, 2004; 117(20): 4863 - 4871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. W. Zimmerman, J. M. D. T. Nelissen, S. E. van Emst-de Vries, P. H. G. M. Willems, F. de Lange, J. G. Collard, F. N. van Leeuwen, and C. G. Figdor Cytoskeletal restraints regulate homotypic ALCAM-mediated adhesion through PKC{alpha} independently of Rho-like GTPases J. Cell Sci., June 1, 2004; 117(13): 2841 - 2852. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Fujiwara, K. Tatsumi, K. Kosaka, Y. Sato, T. Higuchi, S. Yoshioka, M. Maeda, M. Ueda, and S. Fujii Human Blastocysts and Endometrial Epithelial Cells Express Activated Leukocyte Cell Adhesion Molecule (ALCAM/CD166) J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 3437 - 3443. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-F. Lambert, M. Liu, G. A. Colvin, M. Dooner, C. I. McAuliffe, P. S. Becker, B. G. Forget, S. M. Weissman, and P. J. Quesenberry Marrow Stem Cells Shift Gene Expression and Engraftment Phenotype with Cell Cycle Transit J. Exp. Med., June 2, 2003; 197(11): 1563 - 1572. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Neef, M. A. Kuske, E. Prols, and J. P. Johnson Identification of the Human PHLDA1/TDAG51 Gene: Down-Regulation in Metastatic Melanoma Contributes to Apoptosis Resistance and Growth Deregulation Cancer Res., October 15, 2002; 62(20): 5920 - 5929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Arai, O. Ohneda, T. Miyamoto, X. Q. Zhang, and T. Suda Mesenchymal Stem Cells in Perichondrium Express Activated Leukocyte Cell Adhesion Molecule and Participate in Bone Marrow Formation J. Exp. Med., June 17, 2002; 195(12): 1549 - 1563. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Li, K. Satyamoorthy, and M. Herlyn DYNAMICS OF CELL INTERACTIONS AND COMMUNICATIONS DURING MELANOMA DEVELOPMENT Crit. Rev. Oral. Biol. Med., January 1, 2002; 13(1): 62 - 70. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Boukerche, P. Baril, E. Tabone, F. Bérard, K. Sanhadji, B. Balme, F. Wolf, H. Perrot, and L. Thomas A New Mr 55,000 Surface Protein Implicated in Melanoma Progression: Association with a Metastatic Phenotype Cancer Res., October 1, 2000; 60(20): 5848 - 5856. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. M. D. T. Nelissen, I. M. Peters, B. G. de Grooth, Y. van Kooyk, and C. G. Figdor Dynamic Regulation of Activated Leukocyte Cell Adhesion Molecule-mediated Homotypic Cell Adhesion through the Actin Cytoskeleton Mol. Biol. Cell, June 1, 2000; 11(6): 2057 - 2068. [Abstract] [Full Text]
|
|
|
|
 |
|
 L. C. L. T. van Kempen, J. J. van den Oord, G. N. P. van Muijen, U. H. Weidle, H. P. J. Bloemers, and G. W. M. Swart Activated Leukocyte Cell Adhesion Molecule/CD166, a Marker of Tumor Progression in Primary Malignant Melanoma of the Skin Am. J. Pathol., March 1, 2000; 156(3): 769 - 774. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Cortes, F. Deschaseaux, N. Uchida, M.-C. Labastie, A. M. Friera, D. He, P. Charbord, and B. Peault HCA, an Immunoglobulin-Like Adhesion Molecule Present on the Earliest Human Hematopoietic Precursor Cells, Is Also Expressed by Stromal Cells in Blood-Forming Tissues Blood, February 1, 1999; 93(3): 826 - 837. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. L. T. van Kempen, J. M. D. T. Nelissen, W. G. J. Degen, R. Torensma, U. H. Weidle, H. P. J. Bloemers, C. G. Figdor, and G. W. M. Swart Molecular Basis for the Homophilic Activated Leukocyte Cell Adhesion Molecule (ALCAM)-ALCAM Interaction J. Biol. Chem., July 6, 2001; 276(28): 25783 - 25790. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
