| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
American Journal of Pathology, Vol 152, 1563-1575, Copyright © 1998 by American Society for Investigative Pathology
REGULAR ARTICLES |
G Chevalier, H Yeger, C Martinerie, M Laurent, J Alami, PN Schofield and B Perbal
Laboratoire d'Oncologie Virale et Moleculaire, INSERM U142 Hopital Saint-Antoine, Paris, France.
We previously established that the expression of the human nov gene (novH) was altered in Wilms' tumors and that levels of novH and WT1 mRNA were inversely correlated in individual Wilms' tumors. Insofar as novH has been shown to be a target for WT1 regulation, novH might play an important role during normal nephrogenesis and in the development of Wilms' tumors. We now show that during normal nephrogenesis novH protein is tightly associated with differentiation of glomerular podocytes. NovH expression is not restricted to renal differentiation but is also detected in endothelium and neural tissue of the kidney. Our results establish that alteration of novH expression in sporadic and heritable Wilms' tumors is associated with dysregulated expression of both novH mRNA and protein. In general, the highest novH expression was noted in the Wilms' tumor, genitourinary anomalies, aniridia, and mental retardation (WAGR)-associated Wilms' tumors. Expression in the Denys-Drash syndrome (DDS)-associated Wilms' tumors fell within the variable spectrum observed in sporadic Wilms' tumor cases. As in developing kidney podocytes, novH protein was also prominent in the abnormal hypoplastic podocytes from DDS cases and in kidney podocytes adjoining Wilms' tumors. In Wilms' tumors exhibiting heterotypic differentiation, novH protein was expressed at high levels in tumor- derived striated muscle and at lower levels in tumor-derived cartilage. These observations taken together indicate that novH may represent both a marker of podocytic differentiation in kidney and a marker of heterotypic mesenchymal differentiation in Wilms' tumors. In addition, absence or very low levels of WT1 are correlated with higher novH expression, and its variable expression in cases with mutant WT1 (sporadic and DDS) suggests that the potential activation and repression transcriptional functions possessed by WT1 are likely dependent on the specific mutation incurred.
This article has been cited by other articles:
 |
|
 |
|
  V. Vallacchi, M. Daniotti, F. Ratti, D. Di Stasi, P. Deho, A. De Filippo, G. Tragni, A. Balsari, A. Carbone, L. Rivoltini, et al. CCN3/Nephroblastoma Overexpressed Matricellular Protein Regulates Integrin Expression, Adhesion, and Dissemination in Melanoma Cancer Res., February 1, 2008; 68(3): 715 - 723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gellhaus, M. Schmidt, C. Dunk, S. J. Lye, and E. Winterhager The Circulating Proangiogenic Factors CYR61 (CCN1) and NOV (CCN3) Are Significantly Decreased in Placentae and Sera of Preeclamptic Patients Reproductive Sciences, December 1, 2007; 14(8_suppl): 46 - 52. [Abstract] [PDF]
|
|
|
|
 |
|
 M. Fukunaga-Kalabis, G. Martinez, Z.-J. Liu, J. Kalabis, P. Mrass, W. Weninger, S. M. Firth, N. Planque, B. Perbal, and M. Herlyn CCN3 controls 3D spatial localization of melanocytes in the human skin through DDR1 J. Cell Biol., November 20, 2006; 175(4): 563 - 569. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Yan, R. C. Baxter, B. Perbal, and S. M. Firth The Aminoterminal Insulin-Like Growth Factor (IGF) Binding Domain of IGF Binding Protein-3 Cannot Be Functionally Substituted by the Structurally Homologous Domain of CCN3 Endocrinology, November 1, 2006; 147(11): 5268 - 5274. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Bariety, C. Mandet, G. S. Hill, and P. Bruneval Parietal Podocytes in Normal Human Glomeruli J. Am. Soc. Nephrol., October 1, 2006; 17(10): 2770 - 2780. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. McCallum, S. Price, N. Planque, B. Perbal, A. Pierce, A. D. Whetton, and A. E. Irvine A novel mechanism for BCR-ABL action: stimulated secretion of CCN3 is involved in growth and differentiation regulation Blood, September 1, 2006; 108(5): 1716 - 1723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Gellhaus, M. Schmidt, C. Dunk, S. J. Lye, R. Kimmig, and E. Winterhager Decreased expression of the angiogenic regulators CYR61 (CCN1) and NOV (CCN3) in human placenta is associatedwith pre-eclampsia Mol. Hum. Reprod., June 1, 2006; 12(6): 389 - 399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. G. Lin, C.-C. Chen, S.-J. Leu, T. M. Grzeszkiewicz, and L. F. Lau Integrin-dependent Functions of the Angiogenic Inducer NOV (CCN3): IMPLICATION IN WOUND HEALING J. Biol. Chem., March 4, 2005; 280(9): 8229 - 8237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Gellhaus, X. Dong, S. Propson, K. Maass, L. Klein-Hitpass, M. Kibschull, O. Traub, K. Willecke, B. Perbal, S. J. Lye, et al. Connexin43 Interacts with NOV: A POSSIBLE MECHANISM FOR NEGATIVE REGULATION OF CELL GROWTH IN CHORIOCARCINOMA CELLS J. Biol. Chem., August 27, 2004; 279(35): 36931 - 36942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Fu, J. F. Bechberger, M. A. Ozog, B. Perbal, and C. C. Naus CCN3 (NOV) Interacts with Connexin43 in C6 Glioma Cells: POSSIBLE MECHANISM OF CONNEXIN-MEDIATED GROWTH SUPPRESSION J. Biol. Chem., August 27, 2004; 279(35): 36943 - 36950. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A W Rachfal, M H Luquette, and D R Brigstock Expression of connective tissue growth factor (CCN2) in desmoplastic small round cell tumour J. Clin. Pathol., April 1, 2004; 57(4): 422 - 425. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J Alami, B R Williams, and H Yeger Differential expression of E-cadherin and {beta} catenin in primary and metastatic Wilms's tumours Mol. Pathol., August 1, 2003; 56(4): 218 - 225. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ratcliffe, M. Nakanishi, and R. B. Jaffe Identification of Definitive and Fetal Zone Markers in the Human Fetal Adrenal Gland Reveals Putative Developmental Genes J. Clin. Endocrinol. Metab., July 1, 2003; 88(7): 3272 - 3277. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. G. Lin, S.-J. Leu, N. Chen, C. M. Tebeau, S.-X. Lin, C.-Y. Yeung, and L. F. Lau CCN3 (NOV) Is a Novel Angiogenic Regulator of the CCN Protein Family J. Biol. Chem., June 20, 2003; 278(26): 24200 - 24208. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Thibout, C. Martinerie, C. Creminon, F. Godeau, P. Boudou, Y. Le Bouc, and M. Laurent Characterization of Human NOV in Biological Fluids: An Enzyme Immunoassay for the Quantification of Human NOV in Sera from Patients with Diseases of the Adrenal Gland and of the Nervous System J. Clin. Endocrinol. Metab., January 1, 2003; 88(1): 327 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lafont, M. Laurent, H. Thibout, F. Lallemand, Y. Le Bouc, A. Atfi, and C. Martinerie The Expression of novH in Adrenocortical Cells Is Down-regulated by TGFbeta 1 through c-Jun in a Smad-independent Manner J. Biol. Chem., October 18, 2002; 277(43): 41220 - 41229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Sakamoto, S. Yamaguchi, R. Ando, A. Miyawaki, Y. Kabasawa, M. Takagi, C. L. Li, B. Perbal, and K.-i. Katsube The Nephroblastoma Overexpressed Gene (NOV/ccn3) Protein Associates with Notch1 Extracellular Domain and Inhibits Myoblast Differentiation via Notch Signaling Pathway J. Biol. Chem., August 9, 2002; 277(33): 29399 - 29405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C L Li, V Martinez, B He, A Lombet, and B Perbal A role for CCN3 (NOV) in calcium signalling Mol. Pathol., August 1, 2002; 55(4): 250 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Manara, B. Perbal, S. Benini, R. Strammiello, V. Cerisano, S. Perdichizzi, M. Serra, A. Astolfi, F. Bertoni, J. Alami, et al. The Expression of ccn3(nov) Gene in Musculoskeletal Tumors Am. J. Pathol., March 1, 2002; 160(3): 849 - 859. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Desnoyers, D. Arnott, and D. Pennica WISP-1 Binds to Decorin and Biglycan J. Biol. Chem., December 7, 2001; 276(50): 47599 - 47607. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Gupta, H Wang, T L McLeod, C C G Naus, S Kyurkchiev, S Advani, J Yu, B Perbal, and R R Weichselbaum Inhibition of glioma cell growth and tumorigenic potential by CCN3 (NOV) Mol. Pathol., October 1, 2001; 54(5): 293 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Martinerie, C. Gicquel, A. Louvel, M. Laurent, P. N. Schofield, and Y. Le Bouc Altered Expression of novH Is Associated with Human Adrenocortical Tumorigenesis J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 3929 - 3940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M Maillard, B Cadot, R Y Ball, K Sethia, D R Edwards, B Perbal, and R Tatoud Differential expression of the ccn3 (nov) proto-oncogene in human prostate cell lines and tissues Mol. Pathol., August 1, 2001; 54(4): 275 - 280. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B-Y Su, W-Q Cai, C-G Zhang, V Martinez, A Lombet, and B Perbal The expression of ccn3 (nov) RNA and protein in the rat central nervous system is developmentally regulated Mol. Pathol., June 1, 2001; 54(3): 184 - 191. [Abstract] [Full Text]
|
|
|
|
|
|
B Perbal NOV (nephroblastoma overexpressed) and the CCN family of genes: structural and functional issues Mol. Pathol., April 1, 2001; 54(2): 57 - 79. [Abstract] [Full Text]
|
|
|
|
|
|
B Perbal The CCN family of genes: a brief history Mol. Pathol., April 1, 2001; 54(2): 103 - 104. [Full Text]
|
|
|
|
 |
|
 P. D. Ellis, Q. Chen, P. J. Barker, J. C. Metcalfe, and P. R. Kemp Nov Gene Encodes Adhesion Factor for Vascular Smooth Muscle Cells and Is Dynamically Regulated in Response to Vascular Injury Arterioscler. Thromb. Vasc. Biol., August 1, 2000; 20(8): 1912 - 1919. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Hwa, Y. Oh, and R. G. Rosenfeld The Insulin-Like Growth Factor-Binding Protein (IGFBP) Superfamily Endocr. Rev., December 1, 1999; 20(6): 761 - 787. [Abstract] [Full Text]
|
|
|
|
|
|
D. R. Brigstock The Connective Tissue Growth Factor/Cysteine- Rich 61/Nephroblastoma Overexpressed (CCN) Family Endocr. Rev., April 1, 1999; 20(2): 189 - 206. [Abstract] [Full Text]
|
|
|
|
|
|
Christine P. Burren, E. M. Wilson, V. Hwa, Y. Oh, and R. G. Rosenfeld Binding Properties and Distribution of Insulin-Like Growth Factor Binding Protein-Related Protein 3 (IGFBP-rP3/NovH), an Additional Member of the IGFBP Superfamily J. Clin. Endocrinol. Metab., March 1, 1999; 84(3): 1096 - 1103. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Perbal, C. Martinerie, R. Sainson, M. Werner, B. He, and B. Roizman The C-terminal domain of the regulatory protein NOVH is sufficient to promote interaction with fibulin 1C: A clue for a role of NOVH in cell-adhesion signaling PNAS, February 2, 1999; 96(3): 869 - 874. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Albrecht, H. von der Kammer, M. Mayhaus, J. Klaudiny, M. Schweizer, and R. M. Nitsch Muscarinic Acetylcholine Receptors Induce the Expression of the Immediate Early Growth Regulatory Gene CYR61 J. Biol. Chem., September 8, 2000; 275(37): 28929 - 28936. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
