help button home button Am J Pathol Epitomics, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hakulinen, J.
Right arrow Articles by Meri, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hakulinen, J.
Right arrow Articles by Meri, S.
(American Journal of Pathology. 1998;153:845-855.)
© 1998 American Society for Investigative Pathology

Regular Articles

Complement-Mediated Killing of Microtumors in Vitro

Juha Hakulinen and Seppo Meri

From the Department of Bacteriology and Immunology, Haartman Institute, University of Helsinki, Helsinki, Finland

Complement-mediated lysis of cancer cells growing in three-dimensional aggregates involves factors that are not associated with the killing of cells in suspension. We have used multicellular tumor spheroids established from breast carcinoma (T47D) and ovarian teratocarcinoma (PA-1) cell lines as models to study complement-mediated destruction of micrometastases and small solid tumors. We found that significant killing of microtumors treated with an antitumor antibody and a specific monoclonal antibody (YTH53.1) against the complement lysis inhibitor protectin (CD59) started to occur after a 1 to 2-hour lag phase. After an overnight incubation, the microtumors became totally infiltrated by the YTH53.1 monoclonal antibody and C1q, whereas C3 and C5b-9 penetrated as a frontier to the peripheral cell layers. A 51Cr release assay showed that during a 24-hour pulsed treatment with complement, 33% of cells in the spheroids were killed, and the average tumor volume decreased by 28%. According to propidium iodide staining, complement exposure resulted in killing and peeling off of the outermost tumor cells.




This article has been cited by other articles:


Home page
 Cancer Res.Home page
S. K. Green, M. C. I. Karlsson, J. V. Ravetch, and R. S. Kerbel
Disruption of Cell-Cell Adhesion Enhances Antibody-dependent Cellular Cytotoxicity: Implications for Antibody-based Therapeutics of Cancer
Cancer Res., December 1, 2002; 62(23): 6891 - 6900.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pathol.Home page
T Inoue, M Yamakawa, and T Takahashi
Expression of complement regulating factors in gastric cancer cells
Mol. Pathol., June 1, 2002; 55(3): 193 - 199.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. A. Lindorfer, A. Nardin, P. L. Foley, M. D. Solga, A. J. Bankovich, E. N. Martin, A. L. Henderson, C. W. Price, E. Gyimesi, C. P. Wozencraft, et al.
Targeting of Pseudomonas aeruginosa in the Bloodstream with Bispecific Monoclonal Antibodies
J. Immunol., August 15, 2001; 167(4): 2240 - 2249.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
M. Redondo, E. Villar, J. Torres-Munoz, T. Tellez, M. Morell, and C. K. Petito
Overexpression of Clusterin in Human Breast Carcinoma
Am. J. Pathol., August 1, 2000; 157(2): 393 - 399.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Society for Investigative Pathology.