This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Shiokawa, S. Articles by Yamamoto, K. Search for Related Content PubMed PubMed Citation Articles by Shiokawa, S. Articles by Yamamoto, K.

(American Journal of Pathology. 1998;153:1393-1400.)
© 1998 American Society for Investigative Pathology

Technical Advances

Establishment of a Novel B Cell Clonality Analysis Using Single-Strand Conformation Polymorphism of Immunoglobulin Light Chain Messenger Signals

Satoshi Shiokawa* , Junji Nishimura , Kouichi Ohshima , Naokuni Uike§ and Kazuhiko Yamamoto*

From the Department of Clinical Immunology,* Medical Institute of Bioregulation, Kyushu University, Beppu, Oita, the Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, the Department of Pathology, School of Medicine, Fukuoka University, and the Department of Hematology,§ National Kyushu Cancer Center Hospital, Fukuoka, Fukuoka, Japan

The remarkable diversity of the complementarity determining region (CDR) 3 of the immunoglobulin (Ig) heavy (H) chain gene rearrangements has been exploited to identify the clonal populations of B cells in B cell malignancies. However, when B cell malignancies of different categories were examined, the overall detection rate was found to be approximately 70%. The development of a simple clonality analysis using Ig light (L) chain CDR3 diversity has been hampered due to the sparseness of knowledge regarding the sequence of V and V gene segments and the restriction of L chain CDR3 length. Based on the recently reported V and V gene sequences, we designed V and V framework 3 consensus primers. We combined the reverse transcriptase polymerase chain reaction (RT-PCR) of IgL chain transcripts with a single-strand conformation polymorphism (SSCP) analysis and then analyzed samples from patients with B cell malignancies. Clonal B cell populations were detected as discrete bands, and identical clones showed a similar mobility in a RT-PCR SSCP analysis. This method was thus found to be a useful supplement to the previously described approach of VH gene amplification for detecting clonal B cell populations. By using SSCP, we were able to determine the clonal identities of B cell expansion in different samples.

This article has been cited by other articles:

				R. Yao, S. A. Rich, and E. Schneider Validation of Sixteen Leukemia and Lymphoma Cell Lines as Controls for Molecular Gene Rearrangement Assays Clin. Chem., August 1, 2002; 48(8): 1344 - 1351. [Abstract] [Full Text] [PDF]

				S Shiokawa, N Matsumoto, and J Nishimura Clonal analysis of B cells in the osteoarthritis synovium Ann Rheum Dis, August 1, 2001; 60(8): 802 - 805. [Abstract] [Full Text] [PDF]

				T. Takano, A. Miyauchi, F. Matsuzuka, H. Yoshida, K. Kuma, and N. Amino Diagnosis of Thyroid Malignant Lymphoma by Reverse Transcription-Polymerase Chain Reaction Detecting the Monoclonality of Immunoglobulin Heavy Chain Messenger Ribonucleic Acid J. Clin. Endocrinol. Metab., February 1, 2000; 85(2): 671 - 675. [Abstract] [Full Text]

				J. Z. Gong, S. Zheng, R. Chiarle, C. De Wolf-Peeters, G. Palestro, G. Frizzera, and G. Inghirami Detection of Immunoglobulin {kappa} Light Chain Rearrangements by Polymerase Chain Reaction : An Improved Method for Detecting Clonal B-CellLymphoproliferative Disorders Am. J. Pathol., August 1, 1999; 155(2): 355 - 363. [Abstract] [Full Text] [PDF]