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(American Journal of Pathology. 1998;153:1597-1607.)
© 1998 American Society for Investigative Pathology

Regular Articles

H19 Overexpression in Breast Adenocarcinoma Stromal Cells Is Associated with Tumor Values and Steroid Receptor Status but Independent of p53 and Ki-67 Expression

Eric Adriaenssens* , Lionel Dumont* , Séverine Lottin* , Domitille Bolle* , Alain Leprêtre , Alice Delobelle , Fatima Bouali§ , Thierry Dugimont*|| , Jean Coll¶ and Jean-Jacques Curgy*

From the Centre de Biologie Cellulaire,* Unité Dynamique des Cellules Embryonnaires et Cancéreuses, and Laboratoire d'Ecologie Numérique, Université des Sciences et Technologies de Lille, Villeneuve d'Ascq, and the Laboratoire d'Anatomie et de Cytologie Pathologique, Centre Oscar Lambret, and Régulation des Processus Invasifs, de l'Angiogenèse et de l'Apoptose§ and Immunopathologie Cellulaire des Maladies Infectueuses,^¶ Institut de Biologie Moléculaire, Institut Pasteur, Lille, France; and Faculté des Sciences Jean Perrin,^|| Universitré d'Antois, Lens, France

In a previous study we described the expression of the H19 gene by in situ hybridization (ISH) in normal breast and in benign or malignant breast tumors (Dugimont T, Curgy JJ, Wernert N, Delobelle A, Raes MB, Joubel A, Stéhelin D, Coll J: Biol Cell 1995, 85:117–124). In the present work, 1) we extend the previous one to a statistically useful number of adenocarcinomas, including 10 subclasses, 2) we provide information on the precise ISH localization of the H19 RNA by using, on serial tissue sections, antibodies delineating specifically the stromal or the epithelial component of the breast, and 3) we consider relationships between the H19 gene expression and various clinicopathological information as tumor values (T0 to T4), grades, steroid receptors, lymph node status, and molecular features as the p53 gene product and the Ki-67/MIB-1 protein, which is specific to proliferating cells. Data indicate that 1) in 72.5% of studied breast adenocarcinomas an overall H19 gene expression is increased when compared with healthy tissues, 2) the H19 gene is generally overexpressed in stromal cells (92.2%) and rarely in epithelial cells (2.9% only), 3) an up-regulation of the H19 gene is significantly correlated with the tumor values and the presence of both estrogen and progesterone receptors, and 4) at the cellular level, the H19 gene demonstrates an independent expression versus accumulation of both the p53 protein and the Ki-67/MIB-1 cell-cycle marker.

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