help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Matolcsy, A.
Right arrow Articles by Knowles, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matolcsy, A.
Right arrow Articles by Knowles, D. M.
(American Journal of Pathology. 1998;153:1609-1614.)
© 1998 American Society for Investigative Pathology

Regular Articles

Immunoglobulin VH Gene Mutational Analysis Suggests that Primary Effusion Lymphomas Derive from Different Stages of B Cell Maturation

András Matolcsy* , Roland G. Nádor{dagger} , Ethel Cesarman{dagger} and Daniel M. Knowles{dagger}

From the Department of Pathology,* University Medical School of Pécs, Hungary, and the Department of Pathology,{dagger} Weill Medical College of Cornell University, New York, New York

Primary effusion lymphoma (PEL) is a recently described distinct subtype of non-Hodgkin's lymphoma associated with infection by the Kaposi's sarcoma-associated herpesvirus, also called human herpesvirus-8. Most cases of PEL are also associated with the Epstein-Barr virus (EBV). In order to better characterize the cellular origin of PEL, we investigated the immunoglobulin (Ig) heavy chain variable region (VH) genes expressed by tumor cells of the BC-1 and BC-3 cell lines derived from PELs and five original PEL specimens. In the six EBV-positive PELs examined, including the BC-1 cell line, the expressed VH gene sequences showed numerous point mutations relative to the putative germline VH gene sequences. In addition, the VH segment of one of these cases showed intraclonal sequence heterogeneity, indicating ongoing somatic mutation. In five cases, the distribution and type of mutations indicated that tumor cells had been selected by antigen. Because somatically mutated Ig genes are expressed by B cells that have reached a germinal center/post-germinal center stage of development, these findings suggest that the PEL cell of origin is a germinal center or post-germinal center B cell in most cases. In contrast, the VH gene segment expressed by tumor cells of the BC-3 cell line, which was originated from an EBV-negative PEL obtained from an HIV-negative patient, was unmutated, suggesting a pre-germinal center B cell origin for tumor cells of this particular PEL cell line. Taken together, these findings suggest that development of PELs may not be restricted to one stage of B cell differentiation and may represent transformation of B cells at different stages of ontogeny.




This article has been cited by other articles:


Home page
 J. Virol.Home page
S. J. Wilson, E. H. Tsao, B. L. J. Webb, H. Ye, L. Dalton-Griffin, C. Tsantoulas, C. V. Gale, M.-Q. Du, A. Whitehouse, and P. Kellam
X Box Binding Protein XBP-1s Transactivates the Kaposi's Sarcoma-Associated Herpesvirus (KSHV) ORF50 Promoter, Linking Plasma Cell Differentiation to KSHV Reactivation from Latency
J. Virol., December 15, 2007; 81(24): 13578 - 13586.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. L. Skalsky, M. A. Samols, K. B. Plaisance, I. W. Boss, A. Riva, M. C. Lopez, H. V. Baker, and R. Renne
Kaposi's Sarcoma-Associated Herpesvirus Encodes an Ortholog of miR-155
J. Virol., December 1, 2007; 81(23): 12836 - 12845.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
M-Q Du, C M Bacon, and P G Isaacson
Kaposi sarcoma-associated herpesvirus/human herpesvirus 8 and lymphoproliferative disorders
J. Clin. Pathol., December 1, 2007; 60(12): 1350 - 1357.
[Abstract] [Full Text] [PDF]

Home page
 The OncologistHome page
Y.-B. Chen, A. Rahemtullah, and E. Hochberg
Primary Effusion Lymphoma
Oncologist, May 1, 2007; 12(5): 569 - 576.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Arguello, S. Paz, E. Hernandez, C. Corriveau-Bourque, L. M. Fawaz, J. Hiscott, and R. Lin
Leukotriene A4 Hydrolase Expression in PEL Cells Is Regulated at the Transcriptional Level and Leads to Increased Leukotriene B4 Production.
J. Immunol., June 1, 2006; 176(11): 7051 - 7061.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
W. Fan, D. Bubman, A. Chadburn, W. J. Harrington Jr., E. Cesarman, and D. M. Knowles
Distinct Subsets of Primary Effusion Lymphoma Can Be Identified Based on Their Cellular Gene Expression Profile and Viral Association
J. Virol., January 15, 2005; 79(2): 1244 - 1251.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
M. G. Alexandrakis, F. H. Passam, D. S. Kyriakou, and D. Bouros
Pleural Effusions in Hematologic Malignancies
Chest, April 1, 2004; 125(4): 1546 - 1555.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Trivedi, K. Takazawa, C. Zompetta, L. Cuomo, E. Anastasiadou, A. Carbone, S. Uccini, F. Belardelli, K. Takada, L. Frati, et al.
Infection of HHV-8+ primary effusion lymphoma cells with a recombinant Epstein-Barr virus leads to restricted EBV latency, altered phenotype, and increased tumorigenicity without affecting TCL1 expression
Blood, January 1, 2004; 103(1): 313 - 316.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. G. Jenner, K. Maillard, N. Cattini, R. A. Weiss, C. Boshoff, R. Wooster, and P. Kellam
Kaposi's sarcoma-associated herpesvirus-infected primary effusion lymphoma has a plasma cell gene expression profile
PNAS, September 2, 2003; 100(18): 10399 - 10404.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
U. Klein, A. Gloghini, G. Gaidano, A. Chadburn, E. Cesarman, R. Dalla-Favera, and A. Carbone
Gene expression profile analysis of AIDS-related primary effusion lymphoma (PEL) suggests a plasmablastic derivation and identifies PEL-specific transcripts
Blood, May 15, 2003; 101(10): 4115 - 4121.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. S. Gordon, C. M. Kanegai, J. R. Doerr, and R. Wall
Somatic hypermutation of the B cell receptor genes B29 (Igbeta , CD79b) and mb1 (Igalpha , CD79a)
PNAS, April 1, 2003; 100(7): 4126 - 4131.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. W. French, C. S. Malone, R. R. Shen, M. Renard, S. E. Henson, M. D. Miner, R. Wall, and M. A. Teitell
Sp1 Transactivation of the TCL1 Oncogene
J. Biol. Chem., January 3, 2003; 278(2): 948 - 955.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M.-Q. Du, T. C. Diss, H. Liu, H. Ye, R. A. Hamoudi, J. Cabecadas, H. Y. Dong, N. L. Harris, J. K. C. Chan, J. W. Rees, et al.
KSHV- and EBV-associated germinotropic lymphoproliferative disorder
Blood, October 16, 2002; 100(9): 3415 - 3418.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
D. V. Ablashi, L. G. Chatlynne, J. E. Whitman Jr., and E. Cesarman
Spectrum of Kaposi's Sarcoma-Associated Herpesvirus, or Human Herpesvirus 8, Diseases
Clin. Microbiol. Rev., July 1, 2002; 15(3): 439 - 464.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
E. Oksenhendler, E. Boulanger, L. Galicier, M.-Q. Du, N. Dupin, T. C. Diss, R. Hamoudi, M.-T. Daniel, F. Agbalika, C. Boshoff, et al.
High incidence of Kaposi sarcoma-associated herpesvirus-related non-Hodgkin lymphoma in patients with HIV infection and multicentric Castleman disease
Blood, April 1, 2002; 99(7): 2331 - 2336.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
D Kamnasaran and D W Cox
Current status of human chromosome 14
J. Med. Genet., February 1, 2002; 39(2): 81 - 90.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
C. L. Perez and S. Rudoy
Anti-CD20 Monoclonal Antibody Treatment of Human Herpesvirus 8-Associated, Body Cavity-Based Lymphoma with an Unusual Phenotype in a Human Immunodeficiency Virus-Negative Patient
Clin. Vaccine Immunol., September 1, 2001; 8(5): 993 - 996.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. S. Malone, M. D. Miner, J. R. Doerr, J. P. Jackson, S. E. Jacobsen, R. Wall, and M. Teitell
CmC(A/T)GG DNA methylation in mature B cell lymphoma gene silencing
PNAS, August 10, 2001; (2001) 181206898.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M.-Q. Du, H. Liu, T. C. Diss, H. Ye, R. A. Hamoudi, N. Dupin, V. Meignin, E. Oksenhendler, C. Boshoff, and P. G. Isaacson
Kaposi sarcoma-associated herpesvirus infects monotypic (IgM{lambda}) but polyclonal naive B cells in Castleman disease and associated lymphoproliferative disorders
Blood, April 1, 2001; 97(7): 2130 - 2136.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Krithivas, D. B. Young, G. Liao, D. Greene, and S. D. Hayward
Human Herpesvirus 8 LANA Interacts with Proteins of the mSin3 Corepressor Complex and Negatively Regulates Epstein-Barr Virus Gene Expression in Dually Infected PEL Cells
J. Virol., October 15, 2000; 74(20): 9637 - 9645.
[Abstract] [Full Text]

Home page
 BloodHome page
F. Pica, A. Volpi, A. Serafino, M. Fraschetti, O. Franzese, and E. Garaci
Autocrine nerve growth factor is essential for cell survival and viral maturation in HHV-8-infected primary effusion lymphoma cells
Blood, May 1, 2000; 95(9): 2905 - 2912.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
N. Dupin, T. L. Diss, P. Kellam, M. Tulliez, M.-Q. Du, D. Sicard, R. A. Weiss, P. G. Isaacson, and C. Boshoff
HHV-8 is associated with a plasmablastic variant of Castleman disease that is linked to HHV-8-positive plasmablastic lymphoma
Blood, February 15, 2000; 95(4): 1406 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. K. Muller-Hermelink and A. Greiner
Molecular Analysis of Human Immunoglobulin Heavy Chain Variable Genes (IgVH) in Normal and Malignant B Cells
Am. J. Pathol., November 1, 1998; 153(5): 1341 - 1346.
[Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. S. Malone, M. D. Miner, J. R. Doerr, J. P. Jackson, S. E. Jacobsen, R. Wall, and M. Teitell
CmC(A/T)GG DNA methylation in mature B cell lymphoma gene silencing
PNAS, August 28, 2001; 98(18): 10404 - 10409.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Society for Investigative Pathology.