| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
Receptor Knockout Mice Are Protected from the Fibroproliferative Effects of Inhaled Asbestos Fibers
From the Lung Biology Program*
of the Department
of Pathology and the Department of Medicine,
Tulane University Medical Center, New Orleans, Louisiana
We have demonstrated that C57BL/6–129 hybrid mice with
genes for both the 55kd and 75kd receptors for TNF-
knocked out
(TNF-RKO) fail to develop fibroproliferative lesions after asbestos
exposure. There is good evidence that TNF- plays a major role in
mediating interstitial pulmonary fibrosis. Our findings support this
view and we present here new data obtained by in situ
hybridization showing that expression of the genes coding for
transforming growth factor (TGF-) and platelet-derived growth
factor A-chain (PDGF-A) is reduced in the TNF-RKO mice compared with
control animals. In accordance with this observation, data on
bromodeoxyuridine (BrdU) incorporation in the lungs of the TNF-RKO
mice show no increases over unexposed control animals. In
contrast, wild-type control mice exposed to asbestos exhibit
15- to 20-fold increases in BrdU uptake and consequently develop
fibrogenic lesions. Even though the levels of TNF- gene expression
and protein production were increased in the asbestos-exposed
TNF-RKO mice, the lack of receptor signaling protected the
mice from developing fibroproliferative lesions. We agree with the view
that TNF- is essential for the development of interstitial pulmonary
fibrosis and postulate that TNF- mediates its effects through
activation of other growth factors such as PDGF and TGF- that
control cell growth and matrix production.
This article has been cited by other articles:
 |
|
 |
|
  J. Levis, R. Loi, K. J. Butnor, P. Vacek, C. Steele, B. T. Mossman, and D. J. Weiss Decreased Asbestos-Induced Lung Inflammation and Fibrosis after Radiation and Bone Marrow Transplant Am. J. Respir. Cell Mol. Biol., January 1, 2008; 38(1): 16 - 25. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Spees, D. A. Pociask, D. E. Sullivan, M. J. Whitney, J. A. Lasky, D. J. Prockop, and A. R. Brody Engraftment of Bone Marrow Progenitor Cells in a Rat Model of Asbestos-Induced Pulmonary Fibrosis Am. J. Respir. Crit. Care Med., August 15, 2007; 176(4): 385 - 394. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Fujita, S. Saika, W. W.-Y. Kao, K. Fujita, T. Miyamoto, K. Ikeda, Y. Nakajima, and Y. Ohnishi Endogenous TNF{alpha} Suppression of Neovascularization in Corneal Stroma in Mice Invest. Ophthalmol. Vis. Sci., July 1, 2007; 48(7): 3051 - 3055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yang, M. Bocchetta, B. Kroczynska, A. G. Elmishad, Y. Chen, Z. Liu, C. Bubici, B. T. Mossman, H. I. Pass, J. R. Testa, et al. TNF-{alpha} inhibits asbestos-induced cytotoxicity via a NF-{kappa}B-dependent pathway, a possible mechanism for asbestos-induced oncogenesis PNAS, July 5, 2006; 103(27): 10397 - 10402. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Saika, K. Ikeda, O. Yamanaka, K. C. Flanders, Y. Okada, T. Miyamoto, A. Kitano, A. Ooshima, Y. Nakajima, Y. Ohnishi, et al. Loss of Tumor Necrosis Factor {alpha} Potentiates Transforming Growth Factor {beta}-mediated Pathogenic Tissue Response during Wound Healing Am. J. Pathol., June 1, 2006; 168(6): 1848 - 1860. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Agostini and C. Gurrieri Chemokine/Cytokine cocktail in idiopathic pulmonary fibrosis. Proceedings of the ATS, January 1, 2006; 3(4): 357 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Sullivan, M. Ferris, D. Pociask, and A. R. Brody Tumor Necrosis Factor-{alpha} Induces Transforming Growth Factor-{beta}1 Expression in Lung Fibroblasts Through the Extracellular Signal-Regulated Kinase Pathway Am. J. Respir. Cell Mol. Biol., April 1, 2005; 32(4): 342 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Fujita, J. M. Shannon, O. Morikawa, J. Gauldie, N. Hara, and R. J. Mason Overexpression of Tumor Necrosis Factor-{alpha} Diminishes Pulmonary Fibrosis Induced by Bleomycin or Transforming Growth Factor-{beta} Am. J. Respir. Cell Mol. Biol., December 1, 2003; 29(6): 669 - 676. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kaminski, J. A. Belperio, P. B. Bitterman, L. Chen, S. W. Chensue, A. M.K. Choi, S. Dacic, J. H. Dauber, R. M. du Bois, J. J. Enghild, et al. Idiopathic Pulmonary Fibrosis Am. J. Respir. Cell Mol. Biol., September 1, 2003; 29(3): S1 - 105. [Full Text] [PDF]
|
|
|
|
 |
|
 C. Jakubzick, E. S. Choi, B. H. Joshi, M. P. Keane, S. L. Kunkel, R. K. Puri, and C. M. Hogaboam Therapeutic Attenuation of Pulmonary Fibrosis Via Targeting of IL-4- and IL-13-Responsive Cells J. Immunol., September 1, 2003; 171(5): 2684 - 2693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Warshamana, D. A. Pociask, P. Sime, D. A. Schwartz, and A. R. Brody Susceptibility to Asbestos-Induced and Transforming Growth Factor-{beta}1-Induced Fibroproliferative Lung Disease in Two Strains of Mice Am. J. Respir. Cell Mol. Biol., December 1, 2002; 27(6): 705 - 713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Churg, J. Dai, H. Tai, C. Xie, and J. L. Wright Tumor Necrosis Factor-{alpha} Is Central to Acute Cigarette Smoke-induced Inflammation and Connective Tissue Breakdown Am. J. Respir. Crit. Care Med., September 15, 2002; 166(6): 849 - 854. [Abstract] [Full Text]
|
|
|
|
|
|
J. C. Bonner, A. B. Rice, J. L. Ingram, C. R. Moomaw, A. Nyska, A. Bradbury, A. R. Sessoms, P. C. Chulada, D. L. Morgan, D. C. Zeldin, et al. Susceptibility of Cyclooxygenase-2-Deficient Mice to Pulmonary Fibrogenesis Am. J. Pathol., August 1, 2002; 161(2): 459 - 470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-Y. Liu, P. J. Sime, T. Wu, G. S. Warshamana, D. Pociask, S.-Y. Tsai, and A. R. Brody Transforming Growth Factor-beta 1 Overexpression in Tumor Necrosis Factor-alpha Receptor Knockout Mice Induces Fibroproliferative Lung Disease Am. J. Respir. Cell Mol. Biol., July 1, 2001; 25(1): 3 - 7. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G.M. Verleden, R.M. du Bois, D. Bouros, M. Drent, A. Millar, J. Muller-Quernheim, G. Semenzato, S. Johnson, G. Sourvinos, D. Olivieri, et al. Genetic predisposition and pathogenetic mechanisms of interstitial lung diseases of unknown origin Eur. Respir. J., July 1, 2001; 18(32_suppl): 17S - 29s. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P.J.A. Borm and R.P.F. Schins Genotype and phenotype in susceptibility to coal workers' pneumoconiosis. The use of cytokines in perspective Eur. Respir. J., July 1, 2001; 18(32_suppl): 127S - 133s. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. Brody, G. S. Warshamana, Jing-Yao, and D. A. Pociask Expression of Transforming Growth Factor-{beta} Induces Fibroproliferative Pulmonary Disease in Fibrosis-Resistant Mice Chest, July 1, 2001; 120(2007): 48S - 49S. [Full Text] [PDF]
|
|
|
|
|
|
P. PANTELIDIS, G. C. FANNING, A. U. WELLS, K. I. WELSH, and R. M. DU BOIS Analysis of Tumor Necrosis Factor-{alpha}, Lymphotoxin-{alpha}, Tumor Necrosis Factor Receptor II, and Interleukin-6 Polymorphisms in Patients with Idiopathic Pulmonary Fibrosis Am. J. Respir. Crit. Care Med., May 1, 2001; 163(6): 1432 - 1436. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. F. Nagueh, S. J. Stetson, N. M. Lakkis, D. Killip, A. Perez-Verdia, M. L. Entman, W. H. Spencer III, and G. Torre-Amione Decreased Expression of Tumor Necrosis Factor-{{alpha}} and Regression of Hypertrophy After Nonsurgical Septal Reduction Therapy for Patients With Hypertrophic Obstructive Cardiomyopathy Circulation, April 10, 2001; 103(14): 1844 - 1850. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Dai and A. Churg Relationship of Fiber Surface Iron and Active Oxygen Species to Expression of Procollagen, PDGF-A, and TGF-{beta}1 in Tracheal Explants Exposed to Amosite Asbestos Am. J. Respir. Cell Mol. Biol., April 1, 2001; 24(4): 427 - 435. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Xie, A. Reusse, J. Dai, K. Zay, J. Harnett, and A. Churg TNF-alpha increases tracheal epithelial asbestos and fiberglass binding via a NF-kappa B-dependent mechanism Am J Physiol Lung Cell Mol Physiol, September 1, 2000; 279(3): L608 - L614. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Abraham, X. Shiwen, C. M. Black, S. Sa, Y. Xu, and A. Leask Tumor Necrosis Factor alpha Suppresses the Induction of Connective Tissue Growth Factor by Transforming Growth Factor-beta in Normal and Scleroderma Fibroblasts J. Biol. Chem., May 12, 2000; 275(20): 15220 - 15225. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. F. Robledo, S. A. Buder-Hoffmann, A. B. Cummins, E. S. Walsh, D. J. Taatjes, and B. T. Mossman Increased Phosphorylated Extracellular Signal-Regulated Kinase Immunoreactivity Associated with Proliferative and Morphologic Lung Alterations after Chrysotile Asbestos Inhalation in Mice Am. J. Pathol., April 1, 2000; 156(4): 1307 - 1316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. Ortiz, J. A. Lasky, H. Safah, M. Reyes, A. Miller, G. Lungarella, and M. Friedman Exacerbation of bleomycin-induced lung injury in mice by amifostine Am J Physiol Lung Cell Mol Physiol, December 1, 1999; 277(6): L1239 - L1244. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Morris and A. R. Brody Stressing Fibrogenesis in Cell Culture Am. J. Respir. Cell Mol. Biol., October 1, 1999; 21(4): 447 - 448. [Full Text]
|
|
|
|
|
|
D. M. Brass, G. W. Hoyle, H. G. Poovey, J.-Y. Liu, and A. R. Brody Reduced Tumor Necrosis Factor-{alpha} and Transforming Growth Factor-ß1 Expression in the Lungs of Inbred Mice that Fail to Develop Fibroproliferative Lesions Consequent to Asbestos Exposure Am. J. Pathol., March 1, 1999; 154(3): 853 - 862. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
