help button home button Am J Pathol International Conference on Pathology of Chest Diseases
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Engelhardt, E.
Right arrow Articles by Gillitzer, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Engelhardt, E.
Right arrow Articles by Gillitzer, R.
(American Journal of Pathology. 1998;153:1849-1860.)
© 1998 American Society for Investigative Pathology

Regular Articles

Chemokines IL-8, GRO{alpha}, MCP-1, IP-10, and Mig Are Sequentially and Differentially Expressed During Phase-Specific Infiltration of Leukocyte Subsets in Human Wound Healing

Eva Engelhardt* , Atiye Toksoy* , Matthias Goebeler* , Sebastian Debus{dagger} , Eva-Bettina Bröcker* and Reinhard Gillitzer*

From the Departments of Dermatology* and Surgery,{dagger} University of Würzburg Medical School, Würzburg, Germany

Healing of cutaneous wounds requires a complex integrated network of repair mechanisms, including the action of newly recruited leukocytes. Using a skin repair model in adult humans, we investigated the role chemokines play in sequential infiltration of leukocyte subsets during wound healing. At day 1 after injury, the C-X-C chemokines IL-8 and growth-related oncogene {alpha} are maximally expressed in the superficial wound bed and are spatially and temporally associated with neutrophil infiltration. IL-8 and growth-related oncogene {alpha} profiles also correlate with keratinocyte migration and subsequently subside after wound closure at day 4. Macrophage infiltration reaches the highest levels at day 2 and is paralleled by monocyte chemoattractant protein-1 mRNA expression in both the basal layer of the proliferative epidermis at the wound margins and mononuclear cells in the wound area. Other monocyte-attracting chemokines such as monocyte chemoattractant protein-3, macrophage inflammatory protein-1{alpha} and -1ß, RANTES, and I309 are undetectable. At day 4, perivascular focal lymphocyte accumulation correlates with strong focal expression of the C-X-C chemokines Mig and IP-10. Our results suggest that a dynamic set of chemokines contributes to the spatially and temporally different infiltration of leukocyte subsets and thus integrates the inflammatory and reparative processes during wound repair.




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
C. C. Yates, D. Whaley, A. Y-Chen, P. Kulesekaran, P. A. Hebda, and A. Wells
ELR-Negative CXC Chemokine CXCL11 (IP-9/I-TAC) Facilitates Dermal and Epidermal Maturation during Wound Repair
Am. J. Pathol., September 1, 2008; 173(3): 643 - 652.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
J. A Belperio and A. Ardehali
Chemokines and Transplant Vasculopathy
Circ. Res., August 29, 2008; 103(5): 454 - 466.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
Y. Yu, Y. Su, S. R. Opalenik, T. Sobolik-Delmaire, N. F. Neel, S. Zaja-Milatovic, S. T. Short, J. Sai, and A. Richmond
Short tail with skin lesion phenotype occurs in transgenic mice with keratin-14 promoter-directed expression of mutant CXCR2
J. Leukoc. Biol., August 1, 2008; 84(2): 406 - 419.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Ishida, J.-L. Gao, and P. M. Murphy
Chemokine Receptor CX3CR1 Mediates Skin Wound Healing by Promoting Macrophage and Fibroblast Accumulation and Function
J. Immunol., January 1, 2008; 180(1): 569 - 579.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
M. Rotondi, L. Chiovato, S. Romagnani, M. Serio, and P. Romagnani
Role of Chemokines in Endocrine Autoimmune Diseases
Endocr. Rev., August 1, 2007; 28(5): 492 - 520.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
M. Frink, A. Lu, B. M. Thobe, Y.-C. Hsieh, M. A. Choudhry, M. G. Schwacha, S. L. Kunkel, and I. H. Chaudry
Monocyte chemoattractant protein-1 influences trauma-hemorrhage-induced distal organ damage via regulation of keratinocyte-derived chemokine production
Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2007; 292(3): R1110 - R1116.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Lung Cell. Mol. Physiol.Home page
M. Frink, B. M. Thobe, Y.-C. Hsieh, M. A. Choudhry, M. G. Schwacha, K. I. Bland, and I. H. Chaudry
17beta-Estradiol inhibits keratinocyte-derived chemokine production following trauma-hemorrhage
Am J Physiol Lung Cell Mol Physiol, February 1, 2007; 292(2): L585 - L591.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Melikoglu, S. Uysal, J. G. Krueger, G. Kaplan, F. Gogus, H. Yazici, and S. Oliver
Characterization of the Divergent Wound-Healing Responses Occurring in the Pathergy Reaction and Normal Healthy Volunteers
J. Immunol., November 1, 2006; 177(9): 6415 - 6421.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
B. W. LeBlanc, J. E. Albina, and J. S. Reichner
The effect of PGG-{beta}-glucan on neutrophil chemotaxis in vivo
J. Leukoc. Biol., April 1, 2006; 79(4): 667 - 675.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
L. Stefanovic, D. A. Brenner, and B. Stefanovic
Direct Hepatotoxic Effect of KC Chemokine in the Liver Without Infiltration of Neutrophils
Experimental Biology and Medicine, September 1, 2005; 230(8): 573 - 586.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Med.Home page
J. M. Jameson, G. Cauvi, L. L. Sharp, D. A. Witherden, and W. L. Havran
{gamma}{delta} T cell-induced hyaluronan production by epithelial cells regulates inflammation
J. Exp. Med., April 18, 2005; 201(8): 1269 - 1279.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
L. Satish, H. C. Blair, A. Glading, and A. Wells
Interferon-Inducible Protein 9 (CXCL11)-Induced Cell Motility in Keratinocytes Requires Calcium Flux-Dependent Activation of {micro}-Calpain
Mol. Cell. Biol., March 1, 2005; 25(5): 1922 - 1941.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
A. M. Tager, R. L. Kradin, P. LaCamera, S. D. Bercury, G. S. V. Campanella, C. P. Leary, V. Polosukhin, L.-H. Zhao, H. Sakamoto, T. S. Blackwell, et al.
Inhibition of Pulmonary Fibrosis by the Chemokine IP-10/CXCL10
Am. J. Respir. Cell Mol. Biol., October 1, 2004; 31(4): 395 - 404.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
T. El-Sawy, M. Miura, and R. Fairchild
Early T Cell Response to Allografts Occuring Prior to Alloantigen Priming Up-Regulates Innate-Mediated Inflammation and Graft Necrosis
Am. J. Pathol., July 1, 2004; 165(1): 147 - 157.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Theilgaard-Monch, S. Knudsen, P. Follin, and N. Borregaard
The Transcriptional Activation Program of Human Neutrophils in Skin Lesions Supports Their Important Role in Wound Healing
J. Immunol., June 15, 2004; 172(12): 7684 - 7693.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
P. Proost, S. Verpoest, K. V. de Borne, E. Schutyser, S. Struyf, W. Put, I. Ronsse, B. Grillet, G. Opdenakker, and J. V. Damme
Synergistic induction of CXCL9 and CXCL11 by Toll-like receptor ligands and interferon-{gamma} in fibroblasts correlates with elevated levels of CXCR3 ligands in septic arthritis synovial fluids
J. Leukoc. Biol., May 1, 2004; 75(5): 777 - 784.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
D. A. Armstrong, J. A. Major, A. Chudyk, and T. A. Hamilton
Neutrophil chemoattractant genes KC and MIP-2 are expressed in different cell populations at sites of surgical injury
J. Leukoc. Biol., April 1, 2004; 75(4): 641 - 648.
[Abstract] [Full Text] [PDF]

Home page
 J Am Coll CardiolHome page
C. W. Lee, E. Stabile, T. Kinnaird, M. Shou, J. M. Devaney, S. E. Epstein, and M. S. Burnett
Temporal patterns of gene expression after acute hindlimb ischemia in mice: insights into the genomic program for collateral vessel development
J. Am. Coll. Cardiol., February 4, 2004; 43(3): 474 - 482.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Strasly, G. Doronzo, P. Capello, D. Valdembri, M. Arese, S. Mitola, P. Moore, G. Alessandri, M. Giovarelli, and F. Bussolino
CCL16 activates an angiogenic program in vascular endothelial cells
Blood, January 1, 2004; 103(1): 40 - 49.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. Klunker, A. Trautmann, M. Akdis, J. Verhagen, P. Schmid-Grendelmeier, K. Blaser, and C. A. Akdis
A Second Step of Chemotaxis After Transendothelial Migration: Keratinocytes Undergoing Apoptosis Release IFN-{gamma}-Inducible Protein 10, Monokine Induced by IFN-{gamma}, and IFN-{gamma}-Inducible {alpha}-Chemoattractant for T Cell Chemotaxis Toward Epidermis in Atopic Dermatitis
J. Immunol., July 15, 2003; 171(2): 1078 - 1084.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. WERNER and R. GROSE
Regulation of Wound Healing by Growth Factors and Cytokines
Physiol Rev, July 1, 2003; 83(3): 835 - 870.
[Abstract] [Full Text] [PDF]

Home page
 Anesth. Analg.Home page
C.-Y. Li, C.-S. Tsai, P.-C. Hsu, C.-T. Wu, C.-S. Wong, and S.-T. Ho
Dobutamine Modulates Lipopolysaccharide-Induced Macrophage Inflammatory Protein-1{alpha} and Interleukin-8 Production in Human Monocytes
Anesth. Analg., July 1, 2003; 97(1): 210 - 215.
[Abstract] [Full Text] [PDF]

Home page
 Arch OphthalmolHome page
U. H. M. Spandau, A. Toksoy, S. Verhaart, R. Gillitzer, and F. E. Kruse
High Expression of Chemokines Gro-{alpha} (CXCL-1), IL-8 (CXCL-8), and MCP-1 (CCL-2) in Inflamed Human Corneas In Vivo
Arch Ophthalmol, June 1, 2003; 121(6): 825 - 831.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. I. Schenk, F. Petersen, H.-D. Flad, and E. Brandt
Platelet-Derived Chemokines CXC Chemokine Ligand (CXCL)7, Connective Tissue-Activating Peptide III, and CXCL4 Differentially Affect and Cross-Regulate Neutrophil Adhesion and Transendothelial Migration
J. Immunol., September 1, 2002; 169(5): 2602 - 2610.
[Abstract] [Full Text] [PDF]

Home page
 Arch DermatolHome page
U. Spandau, E.-B. Brocker, E. Kampgen, and R. Gillitzer
CC and CXC Chemokines Are Differentially Expressed in Erythema Multiforme In Vivo
Arch Dermatol, August 1, 2002; 138(8): 1027 - 1033.
[Abstract] [Full Text] [PDF]

Home page
 NeurologyHome page
J. L. De Bleecker, B. De Paepe, I. E. Vanwalleghem, and J. M. Schroder
Differential expression of chemokines in inflammatory myopathies
Neurology, June 25, 2002; 58(12): 1779 - 1785.
[Abstract] [Full Text] [PDF]

Home page
 ChestHome page
W. I. de Boer
Cytokines and Therapy in COPD* : A Promising Combination?
Chest, May 1, 2002; 121(5_suppl): 209S - 218S.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
H. Shiraha, A. Glading, J. Chou, Z. Jia, and A. Wells
Activation of m-Calpain (Calpain II) by Epidermal Growth Factor Is Limited by Protein Kinase A Phosphorylation of m-Calpain
Mol. Cell. Biol., April 15, 2002; 22(8): 2716 - 2727.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Endlich, D. Armstrong, J. Brodsky, M. Novotny, and T. A. Hamilton
Distinct Temporal Patterns of Macrophage-Inflammatory Protein-2 and KC Chemokine Gene Expression in Surgical Injury
J. Immunol., April 1, 2002; 168(7): 3586 - 3594.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Proost, E. Schutyser, P. Menten, S. Struyf, A. Wuyts, G. Opdenakker, M. Detheux, M. Parmentier, C. Durinx, A.-M. Lambeir, et al.
Amino-terminal truncation of CXCR3 agonists impairs receptor signaling and lymphocyte chemotaxis, while preserving antiangiogenic properties
Blood, December 15, 2001; 98(13): 3554 - 3561.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. Miura, K. Morita, H. Kobayashi, T. A. Hamilton, M. D. Burdick, R. M. Strieter, and R. L. Fairchild
Monokine Induced by IFN-{gamma} Is a Dominant Factor Directing T Cells into Murine Cardiac Allografts During Acute Rejection
J. Immunol., September 15, 2001; 167(6): 3494 - 3504.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
K. Morita, M. Miura, D. R. Paolone, T. M. Engeman, A. Kapoor, D. G. Remick, and R. L. Fairchild
Early Chemokine Cascades in Murine Cardiac Grafts Regulate T Cell Recruitment and Progression of Acute Allograft Rejection
J. Immunol., September 1, 2001; 167(5): 2979 - 2984.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
T. M. Carlos
Leukocyte recruitment at sites of tumor: dissonant orchestration
J. Leukoc. Biol., August 1, 2001; 70(2): 171 - 184.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Q. E. H. Low, I. A. Drugea, L. A. Duffner, D. G. Quinn, D. N. Cook, B. J. Rollins, E. J. Kovacs, and L. A. DiPietro
Wound Healing in MIP-1{alpha}-/- and MCP-1-/- Mice
Am. J. Pathol., August 1, 2001; 159(2): 457 - 463.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
R. Salcedo, H. A. Young, M. L. Ponce, J. M. Ward, H. K. Kleinman, W. J. Murphy, and J. J. Oppenheim
Eotaxin (CCL11) Induces In Vivo Angiogenic Responses by Human CCR3+ Endothelial Cells
J. Immunol., June 15, 2001; 166(12): 7571 - 7578.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Nees, J. M. Geoghegan, T. Hyman, S. Frank, L. Miller, and C. D. Woodworth
Papillomavirus Type 16 Oncogenes Downregulate Expression of Interferon-Responsive Genes and Upregulate Proliferation-Associated and NF-{kappa}B-Responsive Genes in Cervical Keratinocytes
J. Virol., May 1, 2001; 75(9): 4283 - 4296.
[Abstract] [Full Text]

Home page
 J. Leukoc. Biol.Home page
R. Gillitzer and M. Goebeler
Chemokines in cutaneous wound healing
J. Leukoc. Biol., April 1, 2001; 69(4): 513 - 521.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
M. Goebeler, A. Trautmann, A. Voss, E.-B. Brocker, A. Toksoy, and R. Gillitzer
Differential and Sequential Expression of Multiple Chemokines during Elicitation of Allergic Contact Hypersensitivity
Am. J. Pathol., February 1, 2001; 158(2): 431 - 440.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
M. T. Liu, D. Armstrong, T. A. Hamilton, and T. E. Lane
Expression of Mig (Monokine Induced by Interferon-{{gamma}}) Is Important in T Lymphocyte Recruitment and Host Defense Following Viral Infection of the Central Nervous System
J. Immunol., February 1, 2001; 166(3): 1790 - 1795.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
R. SALCEDO, J. H. RESAU, D. HALVERSON, E. A. HUDSON, M. DAMBACH, D. POWELL, K. WASSERMAN, and J. J. OPPENHEIM
Differential expression and responsiveness of chemokine receptors (CXCR1-3) by human microvascular endothelial cells and umbilical vein endothelial cells
FASEB J, October 1, 2000; 14(13): 2055 - 2064.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
M. T. Liu, B. P. Chen, P. Oertel, M. J. Buchmeier, D. Armstrong, T. A. Hamilton, and T. E. Lane
Cutting Edge: The T Cell Chemoattractant IFN-Inducible Protein 10 Is Essential in Host Defense Against Viral-Induced Neurologic Disease
J. Immunol., September 1, 2000; 165(5): 2327 - 2330.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
R. Salcedo, M. L. Ponce, H. A. Young, K. Wasserman, J. M. Ward, H. K. Kleinman, J. J. Oppenheim, and W. J. Murphy
Human endothelial cells express CCR2 and respond to MCP-1: direct role of MCP-1 in angiogenesis and tumor progression
Blood, July 1, 2000; 96(1): 34 - 40.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Beilmann, G. F. Vande Woude, H.-P. Dienes, and P. Schirmacher
Hepatocyte growth factor-stimulated invasiveness of monocytes
Blood, June 15, 2000; 95(12): 3964 - 3969.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Watarai, S. Koga, D. R. Paolone, T. M. Engeman, C. Tannenbaum, T. A. Hamilton, and R. L. Fairchild
Intraallograft Chemokine RNA and Protein During Rejection of MHC-Matched/Multiple Minor Histocompatibility-Disparate Skin Grafts
J. Immunol., June 1, 2000; 164(11): 6027 - 6033.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
H. Shiraha, A. Glading, K. Gupta, and A. Wells
IP-10 Inhibits Epidermal Growth Factor–induced Motility by Decreasing Epidermal Growth Factor Receptor–mediated Calpain Activity
J. Cell Biol., July 12, 1999; 146(1): 243 - 254.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
J. E. Feugate, Q. Li, L. Wong, and M. Martins-Green
The cxc chemokine cCAF stimulates differentiation of fibroblasts into myofibroblasts and accelerates wound closure
J. Cell Biol., January 7, 2002; 156(1): 161 - 172.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the American Society for Investigative Pathology.