| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
From The First Department of Internal Medicine,*
Shinshu
University School of Medicine, Matsumoto, and the Department of
Respiratory Medicine,
Graduate School of
Medicine, Kyoto University, Kyoto, Japan
Inflammatory cells accumulate within the lungs of cigarette smokers. Current concepts suggest that these cells can induce protease-antiprotease and/or oxidant-antioxidant imbalance(s), which may damage the normal lung alveolar and interstitial structures. Because type II pneumocytes line the alveolar space, and because the inflammatory cells migrate and reside at the alveolus, we postulated that the type II pneumocytes might release chemotactic activity for neutrophils and monocytes in response to smoke extract. To test this hypothesis, A549 cells were cultured and the supernatant fluids were evaluated for the neutrophil and monocyte chemotactic activity (NCA and MCA) by a blind-well chamber technique. A549 cells released NCA and MCA in response to smoke extract in a dose- and time-dependent manner (P < 0.05). Checkerboard analysis showed that the activity was chemotactic. Partial characterization of NCA and MCA revealed that the activity was partly heat labile, trypsin sensitive, and ethyl acetate extractable. Lipoxygenase inhibitors and cycloheximide inhibited the release of NCA and MCA. Molecular sieve column chromatography showed multiple peaks for both NCA and MCA. NCA was inhibited by anti-human-interleukin (IL)-8 antibody, granulocyte colony-stimulating factor (G-CSF) antibody, or leukotriene (LT)B4 receptor antagonist. Monocyte chemoattractant protein (MCP)-1 antibody or LTB4 receptor antagonist inhibited MCA. Immunoreactive IL-8, G-CSF, MCP-1, and LTB4 significantly increased in the supernatant fluids in response to smoke extract. These data suggest that the type II pneumocytes may release NCA and MCA and modulate the inflammatory cell recruitment into the lung.
This article has been cited by other articles:
 |
|
 |
|
  R. L. Rouse, G. Murphy, M. J. Boudreaux, D. B. Paulsen, and A. L. Penn Soot Nanoparticles Promote Biotransformation, Oxidative Stress, and Inflammation in Murine Lungs Am. J. Respir. Cell Mol. Biol., August 1, 2008; 39(2): 198 - 207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. F. Chung and I. M. Adcock Multifaceted mechanisms in COPD: inflammation, immunity, and tissue repair and destruction Eur. Respir. J., June 1, 2008; 31(6): 1334 - 1356. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Facchinetti, F. Amadei, P. Geppetti, F. Tarantini, C. Di Serio, A. Dragotto, P. M. Gigli, S. Catinella, M. Civelli, and R. Patacchini {alpha},beta-Unsaturated Aldehydes in Cigarette Smoke Release Inflammatory Mediators from Human Macrophages Am. J. Respir. Cell Mol. Biol., November 1, 2007; 37(5): 617 - 623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. Thatcher, S. B. Maggirwar, C. J. Baglole, H. F. Lakatos, T. A. Gasiewicz, R. P. Phipps, and P. J. Sime Aryl Hydrocarbon Receptor-Deficient Mice Develop Heightened Inflammatory Responses to Cigarette Smoke and Endotoxin Associated with Rapid Loss of the Nuclear Factor-{kappa}B Component RelB Am. J. Pathol., March 1, 2007; 170(3): 855 - 864. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. L. Thirkill, H. Vedagiri, and G. C. Douglas Macaque Trophoblast Migration toward RANTES Is Inhibited by Cigarette Smoke-Conditioned Medium Toxicol. Sci., June 1, 2006; 91(2): 557 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Meyer, L. D. Dwyer-Nield, G. Hurteau, R. L. Keith, Y. Ouyang, B. M. Freed, L. R. Kisley, M. W. Geraci, J. V. Bonventre, R. A. Nemenoff, et al. Attenuation of the pulmonary inflammatory response following butylated hydroxytoluene treatment of cytosolic phospholipase A2 null mice Am J Physiol Lung Cell Mol Physiol, June 1, 2006; 290(6): L1260 - L1266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Berrahmoune, J. V. Lamont, B. Herbeth, P. S. FitzGerald, and S. Visvikis-Siest Biological Determinants of and Reference Values for Plasma Interleukin-8, Monocyte Chemoattractant Protein-1, Epidermal Growth Factor, and Vascular Endothelial Growth Factor: Results from the STANISLAS Cohort Clin. Chem., March 1, 2006; 52(3): 504 - 510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Shinkai, G. H. Foster, and B. K. Rubin Macrolide antibiotics modulate ERK phosphorylation and IL-8 and GM-CSF production by human bronchial epithelial cells Am J Physiol Lung Cell Mol Physiol, January 1, 2006; 290(1): L75 - L85. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. F. Chung The Role of Airway Smooth Muscle in the Pathogenesis of Airway Wall Remodeling in Chronic Obstructive Pulmonary Disease Proceedings of the ATS, November 1, 2005; 2(4): 347 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. H. Thatcher, N. A. McHugh, R. W. Egan, R. W. Chapman, J. A. Hey, C. K. Turner, M. R. Redonnet, K. E. Seweryniak, P. J. Sime, and R. P. Phipps Role of CXCR2 in cigarette smoke-induced lung inflammation Am J Physiol Lung Cell Mol Physiol, August 1, 2005; 289(2): L322 - L328. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Q. Zhang, P. Adiseshaiah, and S. P. Reddy Matrix Metalloproteinase/Epidermal Growth Factor Receptor/Mitogen-Activated Protein Kinase Signaling Regulate fra-1 Induction by Cigarette Smoke in Lung Epithelial Cells Am. J. Respir. Cell Mol. Biol., January 1, 2005; 32(1): 72 - 81. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Laan, S. Bozinovski, and G. P. Anderson Cigarette Smoke Inhibits Lipopolysaccharide-Induced Production of Inflammatory Cytokines by Suppressing the Activation of Activator Protein-1 in Bronchial Epithelial Cells J. Immunol., September 15, 2004; 173(6): 4164 - 4170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. R. Witherden, E. J. Vanden Bon, P. Goldstraw, C. Ratcliffe, U. Pastorino, and T. D. Tetley Primary Human Alveolar Type II Epithelial Cell Chemokine Release: Effects of Cigarette Smoke and Neutrophil Elastase Am. J. Respir. Cell Mol. Biol., April 1, 2004; 30(4): 500 - 509. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Koch, M. Giembycz, K. Ito, S. Lim, E. Jazrawi, P. J. Barnes, I. Adcock, E. Erdmann, and K. F. Chung Mitogen-Activated Protein Kinase Modulation of Nuclear Factor-{kappa}B-Induced Granulocyte Macrophage-Colony-Stimulating Factor Release from Human Alveolar Macrophages Am. J. Respir. Cell Mol. Biol., March 1, 2004; 30(3): 342 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E{-}J.D. Oudijk, J{-}W.J. Lammers, and L. Koenderman Systemic inflammation in chronic obstructive pulmonary disease Eur. Respir. J., November 2, 2003; 22(46_suppl): 5s - 13s. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Churg, R. D. Wang, H. Tai, X. Wang, C. Xie, J. Dai, S. D. Shapiro, and J. L. Wright Macrophage Metalloelastase Mediates Acute Cigarette Smoke-induced Inflammation via Tumor Necrosis Factor-{alpha} Release Am. J. Respir. Crit. Care Med., April 15, 2003; 167(8): 1083 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Koyama, E. Sato, A. Tsukadaira, M. Haniuda, H. Numanami, M. Kurai, S. Nagai, and T. Izumi Vascular endothelial growth factor mRNA and protein expression in airway epithelial cell lines in vitro Eur. Respir. J., December 1, 2002; 20(6): 1449 - 1456. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. K. Russell, A. Thorley, S. V. Culpitt, S. Dodd, L. E. Donnelly, C. Demattos, M. Fitzgerald, and P. J. Barnes Alveolar macrophage-mediated elastolysis: roles of matrix metalloproteinases, cysteine, and serine proteases Am J Physiol Lung Cell Mol Physiol, October 1, 2002; 283(4): L867 - L873. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Churg, K. Zay, S. Shay, C. Xie, S. D. Shapiro, R. Hendricks, and J. L Wright Acute Cigarette Smoke-Induced Connective Tissue Breakdown Requires both Neutrophils and Macrophage Metalloelastase in Mice Am. J. Respir. Cell Mol. Biol., September 1, 2002; 27(3): 368 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Christofidou-Solomidou, S. Kennel, A. Scherpereel, R. Wiewrodt, C. C. Solomides, G. G. Pietra, J.-C. Murciano, S. A. Shah, H. Ischiropoulos, S. M. Albelda, et al. Vascular Immunotargeting of Glucose Oxidase to the Endothelial Antigens Induces Distinct Forms of Oxidant Acute Lung Injury : Targeting to Thrombomodulin, But Not to PECAM-1, Causes Pulmonary Thrombosis and Neutrophil Transmigration Am. J. Pathol., March 1, 2002; 160(3): 1155 - 1169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.F. Chung Cytokines in chronic obstructive pulmonary disease Eur. Respir. J., July 2, 2001; 18(34_suppl): 50S - 59s. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P MAESTRELLI, L RICHELDI, M MORETTI, and L M FABBRI Analysis of sputum in COPD Thorax, June 1, 2001; 56(6): 420 - 422. [Full Text]
|
|
|
|
|
|
I. Rahman and W. MacNee Lung glutathione and oxidative stress: implications in cigarette smoke-induced airway disease Am J Physiol Lung Cell Mol Physiol, December 1, 1999; 277(6): L1067 - L1088. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Sato, S. Koyama, T. Masubuchi, A. Takamizawa, K. Kubo, S. Nagai, and T. Izumi Bleomycin stimulates lung epithelial cells to release neutrophil and monocyte chemotactic activities Am J Physiol Lung Cell Mol Physiol, June 1, 1999; 276(6): L941 - L950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Takamizawa, S. Koyama, E. Sato, T. Masubuchi, K. Kubo, M. Sekiguchi, S. Nagai, and T. Izumi Bleomycin Stimulates Lung Fibroblasts to Release Neutrophil and Monocyte Chemotactic Activity J. Immunol., May 15, 1999; 162(10): 6200 - 6208. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
