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(American Journal of Pathology. 1999;154:495-501.)
© 1999 American Society for Investigative Pathology

Regular Articles

C5a Receptor and Interleukin-6 Are Expressed in Tissue Macrophages and Stimulated Keratinocytes but not in Pulmonary and Intestinal Epithelial Cells

Afshin Fayyazi* , Raoul Sandau , Le Quynh Duong* , Otto Götze , Heinz Joachim Radzun* , Stefan Schweyer* , Afsaneh Soruri and Jörg Zwirner

From the Departments of Pathology* and Immunology, University of Göttingen, Göttingen, Germany

The anaphylatoxin derived from the fifth component of the human complement system (C5a) mediates its effects by binding to a single high-affinity receptor (C5aR/CD88), the expression of which has been traditionally thought to be restricted to granulocytes, monocytes, macrophages (M), and cell lines of myeloid origin. Recent immunohistochemical data suggested that human bronchial and alveolar cells express C5aR as well. To reexamine the tissue distribution of human C5aR expression, transcription of the C5aR gene was investigated in normal and pathologically affected human lung (bronchopneumonia, tuberculosis), large intestine (acute appendicitis, Crohn's disease), and skin (pyogenic granuloma, lichen planus) using in situ hybridization. In contrast to previous evidence, C5aR mRNA could not be detected in pulmonary or intestinal epithelial cells, whereas keratinocytes in inflamed but not in normal skin revealed detectable levels of C5aR transcripts. Additionally, it could be documented that only migrating M express C5aR mRNA, whereas sessile M in normal tissues and epithelioid/multinucleated M found in granulomatous lesions do not. Because C5a has been demonstrated to upregulate the expression of interleukin (IL)-6 in human monocytes, we also studied IL-6 gene transcription in parallel to the C5aR. IL-6 mRNA was detectable in many tissue M. Surprisingly, a tight co-expression of C5aR and IL-6 mRNA was observed in keratinocytes from lesions of pyogenic granuloma and lichen planus. These results point to an as yet unknown role for C5a in the pathogenesis of skin disorders beyond its well-defined function as a chemoattractant and activator of leukocytes.

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