| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
From the National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland
To screen pancreatic carcinomas for chromosomal aberrations we have applied molecular cytogenetic techniques, including fluorescent in situ hybridization, comparative genomic hybridization, and spectral karyotyping to a series of nine established cell lines. Comparative genomic hybridization revealed recurring chromosomal gains on chromosome arms 3q, 5p, 7p, 8q, 12p, and 20q. Chromosome losses were mapped to chromosome arms 8p, 9p, 17p, 18q, 19p, and chromosome 21. The comparison with comparative genomic hybridization data from primary pancreatic tumors indicates that a specific pattern of chromosomal copy number changes is maintained in cell culture. Metaphase chromosomes from six cell lines were analyzed by spectral karyotyping, a technique that allows one to visualize all chromosomes simultaneously in different colors. Spectral karyotyping identified multiple chromosomal rearrangements, the majority of which were unbalanced. No recurring reciprocal translocation was detected. Cytogenetic aberrations were confirmed using fluorescent in situ hybridization with probes for the MDR gene and the tumor suppressor genes p16 and DCC. Copy number increases on chromosome 20q were validated with a probe specific for the nuclear receptor coactivator AIB1 that maps to chromosome 20q12. Amplification of this gene was identified in six of nine pancreatic cancer cell lines and correlated with increased expression.
This article has been cited by other articles:
 |
|
 |
|
  L. Amazit, L. Pasini, A. T. Szafran, V. Berno, R.-C. Wu, M. Mielke, E. D. Jones, M. G. Mancini, C. A. Hinojos, B. W. O'Malley, et al. Regulation of SRC-3 Intercompartmental Dynamics by Estrogen Receptor and Phosphorylation Mol. Cell. Biol., October 1, 2007; 27(19): 6913 - 6932. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. C-K. Chung, S. Zhou, L. Liao, J. C.-Y. Tien, N. M. Greenberg, and J. Xu Genetic Ablation of the Amplified-in-Breast Cancer 1 Inhibits Spontaneous Prostate Cancer Progression in Mice Cancer Res., June 15, 2007; 67(12): 5965 - 5975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Schleicher, C. Poremba, H. Wolters, K.-L. Schafer, N. Senninger, and M. Colombo-Benkmann Gain of Chromosome 8q: A Potential Prognostic Marker in Resectable Adenocarcinoma of the Pancreas? Ann. Surg. Oncol., April 1, 2007; 14(4): 1327 - 1335. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Hossain, M. T. Kuo, and G. F. Saunders Mir-17-5p Regulates Breast Cancer Cell Proliferation by Inhibiting Translation of AIB1 mRNA Mol. Cell. Biol., November 1, 2006; 26(21): 8191 - 8201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mani, A. S. Oh, E. T. Bowden, T. Lahusen, K. L. Lorick, A. M. Weissman, R. Schlegel, A. Wellstein, and A. T. Riegel E6AP Mediates Regulated Proteasomal Degradation of the Nuclear Receptor Coactivator Amplified in Breast Cancer 1 in Immortalized Cells. Cancer Res., September 1, 2006; 66(17): 8680 - 8686. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Igarashi-Migitaka, A. Takeshita, N. Koibuchi, S. Yamada, R. Ohtani-Kaneko, and K. Hirata Differential expression of p160 steroid receptor coactivators in the rat testis and epididymis Eur. J. Endocrinol., October 1, 2005; 153(4): 595 - 604. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. Zhou, J. Yan, W. Luo, G. Ayala, S.-H. Lin, H. Erdem, M. Ittmann, S. Y. Tsai, and M.-J. Tsai SRC-3 Is Required for Prostate Cancer Cell Proliferation and Survival Cancer Res., September 1, 2005; 65(17): 7976 - 7983. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Akada, T. Crnogorac-Jurcevic, S. Lattimore, P. Mahon, R. Lopes, M. Sunamura, S. Matsuno, and N. R. Lemoine Intrinsic Chemoresistance to Gemcitabine Is Associated with Decreased Expression of BNIP3 in Pancreatic Cancer Clin. Cancer Res., April 15, 2005; 11(8): 3094 - 3101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Burwinkel, M. Wirtenberger, R. Klaes, R. K. Schmutzler, E. Grzybowska, A. Forsti, B. Frank, J. L. Bermejo, P. Bugert, B. Wappenschmidt, et al. Association of NCOA3 Polymorphisms with Breast Cancer Risk Clin. Cancer Res., March 15, 2005; 11(6): 2169 - 2174. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. T. Tilli, R. Reiter, A. S. Oh, R. T. Henke, K. McDonnell, G. I. Gallicano, P. A. Furth, and A. T. Riegel Overexpression of an N-Terminally Truncated Isoform of the Nuclear Receptor Coactivator Amplified in Breast Cancer 1 Leads to Altered Proliferation of Mammary Epithelial Cells in Transgenic Mice Mol. Endocrinol., March 1, 2005; 19(3): 644 - 656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. T. Henke, B. R. Haddad, S. E. Kim, J. D. Rone, A. Mani, J. M. Jessup, A. Wellstein, A. Maitra, and A. T. Riegel Overexpression of the Nuclear Receptor Coactivator AIB1 (SRC-3) during Progression of Pancreatic Adenocarcinoma Clin. Cancer Res., September 15, 2004; 10(18): 6134 - 6142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Brennan, Y. Zhang, C. Leo, B. Feng, C. Cauwels, A. J. Aguirre, M. Kim, A. Protopopov, and L. Chin High-Resolution Global Profiling of Genomic Alterations with Long Oligonucleotide Microarray Cancer Res., July 15, 2004; 64(14): 4744 - 4748. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Holzmann, H. Kohlhammer, C. Schwaenen, S. Wessendorf, H. A. Kestler, A. Schwoerer, B. Rau, B. Radlwimmer, H. Dohner, P. Lichter, et al. Genomic DNA-Chip Hybridization Reveals a Higher Incidence of Genomic Amplifications in Pancreatic Cancer than Conventional Comparative Genomic Hybridization and Leads to the Identification of Novel Candidate Genes Cancer Res., July 1, 2004; 64(13): 4428 - 4433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. C. Louie, J. X. Zou, A. Rabinovich, and H.-W. Chen ACTR/AIB1 Functions as an E2F1 Coactivator To Promote Breast Cancer Cell Proliferation and Antiestrogen Resistance Mol. Cell. Biol., June 15, 2004; 24(12): 5157 - 5171. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Aguirre, C. Brennan, G. Bailey, R. Sinha, B. Feng, C. Leo, Y. Zhang, J. Zhang, J. D. Gans, N. Bardeesy, et al. High-resolution characterization of the pancreatic adenocarcinoma genome PNAS, June 15, 2004; 101(24): 9067 - 9072. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Heidenblad, E. F. P. M. Schoenmakers, T. Jonson, L. Gorunova, J. A. Veltman, A. G. van Kessel, and M. Hoglund Genome-Wide Array-Based Comparative Genomic Hybridization Reveals Multiple Amplification Targets and Novel Homozygous Deletions in Pancreatic Carcinoma Cell Lines Cancer Res., May 1, 2004; 64(9): 3052 - 3059. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Goel and R. Janknecht Concerted Activation of ETS Protein ER81 by p160 Coactivators, the Acetyltransferase p300 and the Receptor Tyrosine Kinase HER2/Neu J. Biol. Chem., April 9, 2004; 279(15): 14909 - 14916. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Ghadimi, M. Grade, T. Liersch, C. Langer, A. Siemer, L. Fuzesi, and H. Becker Gain of Chromosome 8q23-24 Is a Predictive Marker for Lymph Node Positivity in Colorectal Cancer Clin. Cancer Res., May 1, 2003; 9(5): 1808 - 1814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. P. Tamm, P. M. Silverman, C. Charnsangavej, and D. B. Evans Diagnosis, Staging, and Surveillance of Pancreatic Cancer Am. J. Roentgenol., May 1, 2003; 180(5): 1311 - 1323. [Full Text] [PDF]
|
|
|
|
 |
|
 V. B. Wreesmann, R. A. Ghossein, S. G. Patel, C. P. Harris, E. A. Schnaser, A. R. Shaha, R. M. Tuttle, J. P. Shah, P. H. Rao, and B. Singh Genome-Wide Appraisal of Thyroid Cancer Progression Am. J. Pathol., November 1, 2002; 161(5): 1549 - 1556. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Platzer, M. B. Upender, K. Wilson, J. Willis, J. Lutterbaugh, A. Nosrati, J. K. V. Willson, D. Mack, T. Ried, and S. Markowitz Silence of Chromosomal Amplifications in Colon Cancer Cancer Res., February 1, 2002; 62(4): 1134 - 1138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Phillips, S. W. Hayward, Y. Wang, J. Vasselli, C. Pavlovich, H. Padilla-Nash, J. R. Pezullo, B. M. Ghadimi, G. D. Grossfeld, A. Rivera, et al. The Consequences of Chromosomal Aneuploidy on Gene Expression Profiles in a Cell Line Model for Prostate Carcinogenesis Cancer Res., November 1, 2001; 61(22): 8143 - 8149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Singh, S. K. Gogineni, P. G. Sacks, A. R. Shaha, J. P. Shah, A. Stoffel, and P. H. Rao Molecular Cytogenetic Characterization of Head and Neck Squamous Cell Carcinoma and Refinement of 3q Amplification Cancer Res., June 1, 2001; 61(11): 4506 - 4513. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Chen, S.-C. M. Wang, B. Hosking, and G. E. O. Muscat Subcellular Localization of the Steroid Receptor Coactivators (SRCs) and MEF2 in Muscle and Rhabdomyosarcoma Cells Mol. Endocrinol., May 1, 2001; 15(5): 783 - 796. [Abstract] [Full Text]
|
|
|
|
|
|
T. L. Sander, A. L. Haas, M. J. Peterson, and J. F. Morris Identification of a Novel SCAN Box-related Protein That Interacts with MZF1B. THE LEUCINE-RICH SCAN BOX MEDIATES HETERO- AND HOMOPROTEIN ASSOCIATIONS J. Biol. Chem., April 21, 2000; 275(17): 12857 - 12867. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Gray and C. Collins Genome changes and gene expression in human solid tumors Carcinogenesis, March 1, 2000; 21(3): 443 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-J. List, K. J. Lauritsen, R. Reiter, C. Powers, A. Wellstein, and A. T. Riegel Ribozyme Targeting Demonstrates That the Nuclear Receptor Coactivator AIB1 Is a Rate-limiting Factor for Estrogen-dependent Growth of Human MCF-7 Breast Cancer Cells J. Biol. Chem., June 22, 2001; 276(26): 23763 - 23768. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
