| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
§
From the Toronto-Sunnybrook Regional Cancer Centre,*
the
Department of Medicine,
University of Toronto,
the Department of Oral Pathology,
Faculty of
Dentistry, University of Toronto, the Departments of Laboratory
Medicine & Dentistry,§
Sunnybrook Health
Science Centre, Toronto, Canada, and the College of Biological
Sciences,¶
University of Minnesota, St.
Paul, Minnesota
At the tissue level, there is experimental and clinical data to suggest a cytokinetic coordination of the cell cycle with a greater proportion of cycling cells entering S-phase and mitosis at specific times of the day. The association of certain cell-cycle proteins with defined events in the cell cycle is well established and may be used to study the timing of cell-cycle phases over 24 hours. In this study oral mucosal biopsies were obtained from six normal human volunteers at 4-hour intervals, six times over 24 hours. Using immunohistochemistry, the number of positive cells expressing the proteins p53, cyclin-E, cyclin-A, cyclin-B1, and Ki-67 was determined for each biopsy and expressed as the number of positive cells per mm of basement membrane. We found a statistically significant circadian variation in the nuclear expression of all of these proteins with the high point of expression for p53 at 10:56 hours, cyclin-E at 14:59 hours, cyclin-A at 16:09 hours, cyclin-B1 at 21:13 hours, and Ki-67 at 02:50 hours. The circadian variation in the nuclear expression of cyclins-E (G1/S phase), -A (G2-phase), and -B1 (M-phase) with a normal physiological progression over time suggests a statistically significant circadian variation in oral epithelial cell proliferation. The finding of a circadian variation in the nuclear expression of p53 protein corresponding to late G1 is novel. This information has clinical implications regarding the timing of chemotherapy and radiotherapy.
This article has been cited by other articles:
 |
|
 |
|
  A. Grechez-Cassiau, B. Rayet, F. Guillaumond, M. Teboul, and F. Delaunay The Circadian Clock Component BMAL1 Is a Critical Regulator of p21WAF1/CIP1 Expression and Hepatocyte Proliferation J. Biol. Chem., February 22, 2008; 283(8): 4535 - 4542. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. A. Martino, N. Tata, G. A. Bjarnason, M. Straume, and M. J. Sole Diurnal protein expression in blood revealed by high throughput mass spectrometry proteomics and implications for translational medicine and body time of day Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2007; 293(3): R1430 - R1437. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.M. Li and F. Levi Circadian Physiology Is a Toxicity Target of the Anticancer Drug Gemcitabine in Mice J Biol Rhythms, April 1, 2007; 22(2): 159 - 166. [Abstract] [PDF]
|
|
|
|
 |
|
 R. W. Garrett and T. A. Gasiewicz The Aryl Hydrocarbon Receptor Agonist 2,3,7,8-Tetrachlorodibenzo-p-dioxin Alters the Circadian Rhythms, Quiescence, and Expression of Clock Genes in Murine Hematopoietic Stem and Progenitor Cells Mol. Pharmacol., June 1, 2006; 69(6): 2076 - 2083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-M. Li, S. Kanekal, D. Crepin, C. Guettier, J. Carriere, G. Elliott, and F. Levi Circadian pharmacology of L-alanosine (SDX-102) in mice. Mol. Cancer Ther., February 1, 2006; 5(2): 337 - 346. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Zhu, T. Zheng, R. G. Stevens, Y. Zhang, and P. Boyle Does "Clock" Matter in Prostate Cancer? Cancer Epidemiol. Biomarkers Prev., January 1, 2006; 15(1): 3 - 5. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Filipski, P. F. Innominato, M. Wu, X.-M. Li, S. Iacobelli, L.-J. Xian, and F. Levi Effects of Light and Food Schedules on Liver and Tumor Molecular Clocks in Mice J Natl Cancer Inst, April 6, 2005; 97(7): 507 - 517. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Canaple, T. Kakizawa, and V. Laudet The Days and Nights of Cancer Cells Cancer Res., November 15, 2003; 63(22): 7545 - 7552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Munoz, M. Brewer, and R. Baler Circadian Transcription. THINKING OUTSIDE THE E-BOX J. Biol. Chem., September 20, 2002; 277(39): 36009 - 36017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Grundschober, F. Delaunay, A. Puhlhofer, G. Triqueneaux, V. Laudet, T. Bartfai, and P. Nef Circadian Regulation of Diverse Gene Products Revealed by mRNA Expression Profiling of Synchronized Fibroblasts J. Biol. Chem., December 7, 2001; 276(50): 46751 - 46758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A. Squier and M. J. Kremer Biology of Oral Mucosa and Esophagus J Natl Cancer Inst Monographs, October 1, 2001; 2001(29): 7 - 15. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Bjarnason, R. C. K. Jordan, P. A. Wood, Q. Li, D. W. Lincoln, R. B. Sothern, W. J. M. Hrushesky, and Y. Ben-David Circadian Expression of Clock Genes in Human Oral Mucosa and Skin : Association with Specific Cell-Cycle Phases Am. J. Pathol., May 1, 2001; 158(5): 1793 - 1801. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Leoncini, C. Bellan, A. Cossu, P. P. Claudio, S. Lazzi, C. Cinti, G. Cevenini, T. Megha, L. Laurini, P. Luzi, et al. Retinoblastoma-related p107 and pRb2/p130 Proteins in Malignant Lymphomas: Distinct Mechanisms of Cell Growth Control Clin. Cancer Res., December 1, 1999; 5(12): 4065 - 4072. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
