help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bussolati, B.
Right arrow Articles by Camussi, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bussolati, B.
Right arrow Articles by Camussi, G.
(American Journal of Pathology. 1999;154:623-632.)
© 1999 American Society for Investigative Pathology

Regular Articles

Interleukin-12 Is Synthesized by Mesangial Cells and Stimulates Platelet-Activating Factor Synthesis, Cytoskeletal Reorganization, and Cell Shape Change

Benedetta Bussolati*{dagger} , Filippo Mariano* , Luigi Biancone{ddagger} , Robert Foà{dagger}§ , Salvatore David{dagger} , Vincenzo Cambi{dagger} and Giovanni Camussi*{ddagger}

From the Laboratorio di Immunopatologia Renale,* Dipartimento di Medicina Interna, and Dipartimento di Scienze Biomediche ed Oncologia Umana,§ Università di Torino, the Cattedra di Nefrologia,{dagger} Università di Parma, and the Dipartimento di Scienze Cliniche e Biologiche,{ddagger} Università dell'Insubria, Italy

Preliminary studies indicate the involvement of interleukin (IL)-12 in experimental renal pathology. In the present study, we evaluated whether cultured glomerular mesangial cells are able to produce IL-12 and whether IL-12 may regulate some of their functions, including the cytoskeletal reorganization, the change in cell shape, and the production of platelet-activating factor (PAF). The results obtained indicate that pro-inflammatory stimuli, such as tumor necrosis factor-{alpha} and bacterial polysaccharides, induce the expression of IL-12 mRNA and the synthesis of the protein by cultured mesangial cells. Moreover, cultured mesangial cells were shown to bind IL-12 and to express the human low-affinity IL-12 ß1-chain receptor. When challenged with IL-12, mesangial cells produced PAF in a dose- and time-dependent manner and superoxide anions. No production of tumor necrosis factor-{alpha} and IL-8 was observed. Moreover, we demonstrate that IL-12 induced a delayed and sustained shape change of mesangial cells that reached its maximum between 90 and 120 minutes of incubation. The changes in cell shape occurred concomitantly with cytoskeletal rearrangements and may be consistent with cell contraction. As IL-12-dependent shape change of mesangial cells was concomitant with the synthesis of PAF, which is known to promote mesangial cell contraction, we investigated the role of PAF using two chemically different PAF receptor antagonists. Both antagonists inhibited almost completely the cell shape change induced by IL-12, whereas they were ineffective on angiotensin-II-induced cell shape change. In conclusion, our results suggest that mesangial cells can either produce IL-12 or be stimulated by this cytokine to synthesize PAF and to undergo shape changes compatible with cell contraction.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
P. Dentelli, A. Rosso, A. Zeoli, R. Gambino, L. Pegoraro, G. Pagano, R. Falcioni, and M. F. Brizzi
Oxidative Stress-mediated Mesangial Cell Proliferation Requires RAC-1/Reactive Oxygen Species Production and beta4 Integrin Expression
J. Biol. Chem., September 7, 2007; 282(36): 26101 - 26110.
[Abstract] [Full Text] [PDF]

Home page
 Stem CellsHome page
M. B. Herrera, S. Bruno, S. Buttiglieri, C. Tetta, S. Gatti, M. C. Deregibus, B. Bussolati, and G. Camussi
Isolation and Characterization of a Stem Cell Population from Adult Human Liver
Stem Cells, December 1, 2006; 24(12): 2840 - 2850.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. J. Brown, H. R. Lock, S. H. Sacks, and M. G. Robson
TLR2 Stimulation of Intrinsic Renal Cells in the Induction of Immune-Mediated Glomerulonephritis
J. Immunol., August 1, 2006; 177(3): 1925 - 1931.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
B. Bussolati, S. Bruno, C. Grange, S. Buttiglieri, M. C. Deregibus, D. Cantino, and G. Camussi
Isolation of Renal Progenitor Cells from Adult Human Kidney
Am. J. Pathol., February 1, 2005; 166(2): 545 - 555.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. Bussolati, G. Peri, G. Salvidio, D. Verzola, A. Mantovani, and G. Camussi
The Long Pentraxin Ptx3 Is Synthesized in IgA Glomerulonephritis and Activates Mesangial Cells
J. Immunol., February 1, 2003; 170(3): 1466 - 1472.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
W. I. Khan, P. A. Blennerhassett, Y. Deng, J. Gauldie, B. A. Vallance, and S. M. Collins
IL-12 gene transfer alters gut physiology and host immunity in nematode-infected mice
Am J Physiol Gastrointest Liver Physiol, July 1, 2001; 281(1): G102 - G110.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
J. R. TIMOSHANKO, A. R. KITCHING, S. R. HOLDSWORTH, and P. G. TIPPING
Interleukin-12 from Intrinsic Cells Is an Effector of Renal Injury in Crescentic Glomerulonephritis
J. Am. Soc. Nephrol., March 1, 2001; 12(3): 464 - 471.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the American Society for Investigative Pathology.