| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Animal Model |
From the Division of Viral Pathogenesis,*
Department of
Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School,
Boston, Massachusetts; the Division of Comparative
Pathology,
New England Regional Primate
Research Center, Harvard Medical School, Southborough, Massachusetts;
the Department of Biochemistry and Molecular
Biology,
University of Hamburg, Hamburg,
Germany; the Bernhard-Nocht-Institute for Tropical
Medicine,§
Hamburg, Germany;
Centocor,¶ Malvern, Pennsylvania, and the
Institute of Immunology,||
Technical University of
Dresden, Dresden, Germany
Nonhuman primates provide valuable animal models for human diseases. However, studies assessing the role of cell-mediated immune responses have been difficult to perform in nonhuman primates. We have shown that CD8+ lymphocyte-mediated immunity in rhesus monkeys can be selectively eliminated using the mouse-human chimeric anti-CD8 monoclonal antibody cM-T807. In vitro, this antibody completely blocked antigen-specific expansion of cytotoxic T cells and decreased major histocompatibility complex class I-restricted, antigen-specific lysis of target cells but did not mediate complement-dependent cell lysis. In vivo administration of cM-T807 in rhesus monkeys resulted in near total depletion of CD8+ T cells from the blood and lymph nodes for up to 6 weeks. This depletion was not solely complement-dependent and persisted longer in adults than in juveniles. Preservation of B cell and CD4+ T cell function in monkeys depleted of CD8+ lymphocytes was demonstrated by their ability to develop humoral immune responses to the administered chimeric monoclonal antibody. Furthermore, during CD8+ lymphocyte depletion, monkeys developed delayed-type hypersensitivity reactions comprised only of CD4+ T cells but not CD8+ T cells. This CD8+ lymphocyte depletion model should prove useful in defining the role of cell-mediated immune responses in controlling infectious diseases in nonhuman primates.
This article has been cited by other articles:
 |
|
 |
|
  E.-Y. Kim, R. S. Veazey, R. Zahn, K. J. McEvers, S. H. C. Baumeister, G. J. Foster, M. D. Rett, M. H. Newberg, M. J. Kuroda, E. P. Rieber, et al. Contribution of CD8+ T Cells to Containment of Viral Replication and Emergence of Mutations in Mamu-A*01-Restricted Epitopes in Simian Immunodeficiency Virus-Infected Rhesus Monkeys J. Virol., June 1, 2008; 82(11): 5631 - 5635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Veazey, P. M. Acierno, K. J. McEvers, S. H. C. Baumeister, G. J. Foster, M. D. Rett, M. H. Newberg, M. J. Kuroda, K. Williams, E.-Y. Kim, et al. Increased Loss of CCR5+ CD45RA- CD4+ T Cells in CD8+ Lymphocyte-Depleted Simian Immunodeficiency Virus-Infected Rhesus Monkeys J. Virol., June 1, 2008; 82(11): 5618 - 5630. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Barry, G. Silvestri, J. T. Safrit, B. Sumpter, N. Kozyr, H. M. McClure, S. I. Staprans, and M. B. Feinberg Depletion of CD8+ Cells in Sooty Mangabey Monkeys Naturally Infected with Simian Immunodeficiency Virus Reveals Limited Role for Immune Control of Virus Replication in a Natural Host Species J. Immunol., June 15, 2007; 178(12): 8002 - 8012. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. VandeWoude and C. Apetrei Going Wild: Lessons from Naturally Occurring T-Lymphotropic Lentiviruses Clin. Microbiol. Rev., October 1, 2006; 19(4): 728 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Dunham, P. Pagliardini, S. Gordon, B. Sumpter, J. Engram, A. Moanna, M. Paiardini, J. N. Mandl, B. Lawson, S. Garg, et al. The AIDS resistance of naturally SIV-infected sooty mangabeys is independent of cellular immunity to the virus Blood, July 1, 2006; 108(1): 209 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Bissel, G. Wang, A. M. Trichel, M. Murphey-Corb, and C. A. Wiley Longitudinal Analysis of Monocyte/Macrophage Infection in Simian Immunodeficiency Virus-Infected, CD8+ T-Cell-Depleted Macaques that Develop Lentiviral Encephalitis Am. J. Pathol., May 1, 2006; 168(5): 1553 - 1569. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Stebbings, N. Berry, H. Waldmann, P. Bird, G. Hale, J. Stott, D. North, R. Hull, J. Hall, J. Lines, et al. CD8+ Lymphocytes Do Not Mediate Protection against Acute Superinfection 20 Days after Vaccination with a Live Attenuated Simian Immunodeficiency Virus J. Virol., October 1, 2005; 79(19): 12264 - 12272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. A. Van Rompay, R. P. Singh, B. Pahar, D. L. Sodora, C. Wingfield, J. R. Lawson, M. L. Marthas, and N. Bischofberger CD8+-Cell-Mediated Suppression of Virulent Simian Immunodeficiency Virus during Tenofovir Treatment J. Virol., May 15, 2004; 78(10): 5324 - 5337. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Harouse, C. Buckner, A. Gettie, R. Fuller, R. Bohm, J. Blanchard, and C. Cheng-Mayer CD8+ T cell-mediated CXC chemokine receptor 4-simian/human immunodeficiency virus suppression in dually infected rhesus macaques PNAS, September 16, 2003; 100(19): 10977 - 10982. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. R. Permar, S. A. Klumpp, K. G. Mansfield, W.-K. Kim, D. A. Gorgone, M. A. Lifton, K. C. Williams, J. E. Schmitz, K. A. Reimann, M. K. Axthelm, et al. Role of CD8+ Lymphocytes in Control and Clearance of Measles Virus Infection of Rhesus Monkeys J. Virol., April 1, 2003; 77(7): 4396 - 4400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Thimme, S. Wieland, C. Steiger, J. Ghrayeb, K. A. Reimann, R. H. Purcell, and F. V. Chisari CD8+ T Cells Mediate Viral Clearance and Disease Pathogenesis during Acute Hepatitis B Virus Infection J. Virol., December 6, 2002; 77(1): 68 - 76. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. E. Burudi, M. C. G. Marcondes, D. D. Watry, M. Zandonatti, M. A. Taffe, and H. S. Fox Regulation of Indoleamine 2,3-Dioxygenase Expression in Simian Immunodeficiency Virus-Infected Monkey Brains J. Virol., October 25, 2002; 76(23): 12233 - 12241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Lifson, J. L. Rossio, M. Piatak Jr., T. Parks, L. Li, R. Kiser, V. Coalter, B. Fisher, B. M. Flynn, S. Czajak, et al. Role of CD8+ Lymphocytes in Control of Simian Immunodeficiency Virus Infection and Resistance to Rechallenge after Transient Early Antiretroviral Treatment J. Virol., November 1, 2001; 75(21): 10187 - 10199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Chung, I. M. Belyakov, M. A. Derby, J. Wang, L. F. Boyd, J. A. Berzofsky, and D. H. Margulies Competitive Inhibition In Vivo and Skewing of the T Cell Repertoire of Antigen-Specific CTL Priming by an Anti-Peptide-MHC Monoclonal Antibody J. Immunol., July 15, 2001; 167(2): 699 - 707. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
