| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
From the Glycobiology Research and Training Center and the Cancer Center, Divisions of Hematology-Oncology and Cellular and Molecular Medicine, University of California San Diego, La Jolla, California
Selectins are adhesion molecules that mediate calcium-dependent cell-cell interactions among leukocytes, platelets, and endothelial cells. The naturally occurring vascular ligands for the selectins are mostly mucin-type glycoproteins. Increased expression and altered glycosylation of mucins are known to be prominent features of carcinoma progression. We have previously shown that all three selectins bind to colon carcinoma cell lines in a calcium-dependent fashion and that carcinoma growth and metastasis formation are attenuated in P-selectin-deficient mice. Here we show that the three recombinant soluble selectins recognize ligands within primary colon carcinoma tissue samples. Affinity chromatography showed that the ligands for all three selectins are O-sialoglycoprotease-sensitive mucins that are recognized in a calcium- and sialic acid-dependent manner. Furthermore, there are separate binding sites on the mucins for each selectin, allowing cross-binding of a single mucin molecule by more than one selectin. We also show that the selectin ligands on purified carcinoma mucins can mediate at least four different pathological interactions among platelets, leukocytes, and endothelial cells. These findings could explain some of the adhesive events of blood-borne tumor cells reported to occur with leukocytes, platelets, and endothelial cells, which are believed to play a part in modulating some early events in tumor metastases.
This article has been cited by other articles:
 |
|
 |
|
  H. Laubli, K.-S. Spanaus, and L. Borsig Selectin-mediated activation of endothelial cells induces expression of CCL5 and promotes metastasis through recruitment of monocytes Blood, November 12, 2009; 114(20): 4583 - 4591. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. N. Thomas, F. Zhu, R. L. Schnaar, C. S. Alves, and K. Konstantopoulos Carcinoembryonic Antigen and CD44 Variant Isoforms Cooperate to Mediate Colon Carcinoma Cell Adhesion to E- and L-selectin in Shear Flow J. Biol. Chem., June 6, 2008; 283(23): 15647 - 15655. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Varki Trousseau's syndrome: multiple definitions and multiple mechanisms Blood, September 15, 2007; 110(6): 1723 - 1729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Borsig, L. Wang, M. C. M. Cavalcante, L. Cardilo-Reis, P. L. Ferreira, P. A. S. Mourao, J. D. Esko, and M. S. G. Pavao Selectin Blocking Activity of a Fucosylated Chondroitin Sulfate Glycosaminoglycan from Sea Cucumber: EFFECT ON TUMOR METASTASIS AND NEUTROPHIL RECRUITMENT J. Biol. Chem., May 18, 2007; 282(20): 14984 - 14991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Yamauchi, M. Yang, P. Jiang, M. Xu, N. Yamamoto, H. Tsuchiya, K. Tomita, A. R. Moossa, M. Bouvet, and R. M. Hoffman Development of Real-time Subcellular Dynamic Multicolor Imaging of Cancer-Cell Trafficking in Live Mice with a Variable-Magnification Whole-Mouse Imaging System. Cancer Res., April 15, 2006; 66(8): 4208 - 4214. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. G. Wahrenbrock and A. Varki Multiple Hepatic Receptors Cooperate to Eliminate Secretory Mucins Aberrantly Entering the Bloodstream: Are Circulating Cancer Mucins the "Tip of the Iceberg"? Cancer Res., February 15, 2006; 66(4): 2433 - 2441. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zipin, M. Israeli-Amit, T. Meshel, O. Sagi-Assif, I. Yron, V. Lifshitz, E. Bacharach, N. I. Smorodinsky, A. Many, P. A. Czernilofsky, et al. Tumor-Microenvironment Interactions: The Fucose-Generating FX Enzyme Controls Adhesive Properties of Colorectal Cancer Cells Cancer Res., September 15, 2004; 64(18): 6571 - 6578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Wei, G. Tai, Y. Gao, N. Li, B. Huang, Y. Zhou, S. Hao, and X. Zeng Modified Heparin Inhibits P-selectin-mediated Cell Adhesion of Human Colon Carcinoma Cells to Immobilized Platelets under Dynamic Flow Conditions J. Biol. Chem., July 9, 2004; 279(28): 29202 - 29210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Ludwig, B. Boehme, M. Podda, R. Henschler, E. Jager, C. Tandi, W.-H. Boehncke, T. M. Zollner, R. Kaufmann, and J. Gille Endothelial P-Selectin as a Target of Heparin Action in Experimental Melanoma Lung Metastasis Cancer Res., April 15, 2004; 64(8): 2743 - 2750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Rump, Y. Morikawa, M. Tanaka, S. Minami, N. Umesaki, M. Takeuchi, and A. Miyajima Binding of Ovarian Cancer Antigen CA125/MUC16 to Mesothelin Mediates Cell Adhesion J. Biol. Chem., March 5, 2004; 279(10): 9190 - 9198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Borsig Selectins Facilitate Carcinoma Metastasis and Heparin Can Prevent Them Physiology, February 1, 2004; 19(1): 16 - 21. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Brown, M. M. Fuster, T. Whisenant, and J. D. Esko Expression Patterns of {alpha}2,3-Sialyltransferases and {alpha}1,3-Fucosyltransferases Determine the Mode of Sialyl Lewis X Inhibition by Disaccharide Decoys J. Biol. Chem., June 20, 2003; 278(26): 23352 - 23359. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. M. Fuster, J. R. Brown, L. Wang, and J. D. Esko A Disaccharide Precursor of Sialyl Lewis X Inhibits Metastatic Potential of Tumor Cells Cancer Res., June 1, 2003; 63(11): 2775 - 2781. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Hanley, O. McCarty, S. Jadhav, Y. Tseng, D. Wirtz, and K. Konstantopoulos Single Molecule Characterization of P-selectin/Ligand Binding J. Biol. Chem., March 14, 2003; 278(12): 10556 - 10561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ozmen, K. N. Ekdahl, G. Elgue, R. Larsson, O. Korsgren, and B. Nilsson Inhibition of Thrombin Abrogates the Instant Blood-Mediated Inflammatory Reaction Triggered by Isolated Human Islets: Possible Application of the Thrombin Inhibitor Melagatran in Clinical Islet Transplantation Diabetes, June 1, 2002; 51(6): 1779 - 1784. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Borsig, R. Wong, R. O. Hynes, N. M. Varki, and A. Varki Synergistic effects of L- and P-selectin in facilitating tumor metastasis can involve non-mucin ligands and implicate leukocytes as enhancers of metastasis PNAS, February 19, 2002; 99(4): 2193 - 2198. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. F. Argibay, J. M. Di Noia, A. Hidalgo, E. Mocetti, M. Barbich, A. S. Lorenti, D. Bustos, M. Tambutti, S. H. Hyon, A. C.C. Frasch, et al. Trypanosoma cruzi surface mucin TcMuc-e2 expressed on higher eukaryotic cells induces human T cell anergy, which is reversible Glycobiology, January 1, 2002; 12(1): 25 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Jadhav, B. S. Bochner, and K. Konstantopoulos Hydrodynamic Shear Regulates the Kinetics and Receptor Specificity of Polymorphonuclear Leukocyte-Colon Carcinoma Cell Adhesive Interactions J. Immunol., November 15, 2001; 167(10): 5986 - 5993. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Borsig, R. Wong, J. Feramisco, D. R. Nadeau, N. M. Varki, and A. Varki Heparin and cancer revisited: Mechanistic connections involving platelets, P-selectin, carcinoma mucins, and tumor metastasis PNAS, March 13, 2001; 98(6): 3352 - 3357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Friederichs, Y. Zeller, A. Hafezi-Moghadam, H.-J. Gröne, K. Ley, and P. Altevogt The CD24/P-selectin Binding Pathway Initiates Lung Arrest of Human A125 Adenocarcinoma Cells Cancer Res., December 1, 2000; 60(23): 6714 - 6722. [Abstract] [Full Text]
|
|
|
|
|
|
M. Ota, N. Takamura, and T. Irimura Involvement of Cell Surface Glycans in Adhesion of Human Colon Carcinoma Cells to Liver Tissue in a Frozen Section Assay: Role of Endo-{beta}-galactosidase-sensitive Structures Cancer Res., September 1, 2000; 60(18): 5261 - 5268. [Abstract] [Full Text]
|
|
|
|
|
|
O. J. T. McCarty, S. A. Mousa, P. F. Bray, and K. Konstantopoulos Immobilized platelets support human colon carcinoma cell tethering, rolling, and firm adhesion under dynamic flow conditions Blood, September 1, 2000; 96(5): 1789 - 1797. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Bruehl, C. R. Bertozzi, and S. D. Rosen Minimal Sulfated Carbohydrates for Recognition by L-selectin and the MECA-79 Antibody J. Biol. Chem., October 13, 2000; 275(42): 32642 - 32648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Perrais, P. Pigny, M.-P. Buisine, N. Porchet, J.-P. Aubert, and I. Van Seuningen-Lempire Aberrant Expression of Human Mucin Gene MUC5B in Gastric Carcinoma and Cancer Cells. IDENTIFICATION AND REGULATION OF A DISTAL PROMOTER J. Biol. Chem., April 27, 2001; 276(18): 15386 - 15396. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
