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(American Journal of Pathology. 1999;155:799-804.)
© 1999 American Society for Investigative Pathology

Regular Articles

Thymosin ß-10 Gene Overexpression Is a General Event in Human Carcinogenesis

Giovanni Santelli^*, Daniela Califano^*, Gennaro Chiappetta^*, Maria Teresa Vento^*, Paola Cannada Bartoli^*, Fulvio Zullo, Francesco Trapasso, Giuseppe Viglietto^* and Alfredo Fusco

From the Istituto dei Tumori di Napoli,^*
Naples, Italy; the Divisione di Ostetricia e Ginecologia,
Facoltà di Medicina e Chirurgia di Catanzaro, Università degli Studi di Catanzaro, Italy; Catanzaro, and the Dipartimento di Medicina Sperimentale e Clinica,
Facoltà di Medicina e Chirurgia di Catanzaro, Università degli Studi di Catanzaro, Catanzaro, Italy

The ß-thymosins comprise a family of structurally related, highly conserved acidic polypeptides, originally isolated from calf thymus. Recently, we have demonstrated the overexpression of thymosin ß-10 (TB10) in rat thyroid transformed cell lines and in human thyroid carcinoma tissues and cell lines. To verify whether TB10 overexpression is a general event in the process of carcinogenesis, we have analyzed TB10 mRNA levels in human colon carcinomas, germ cell tumors of different histological types, breast carcinomas, ovarian carcinomas, uterine carcinomas, colon and esophageal carcinoma cell lines. Overexpression of the TB10 gene was detected in all of the neoplastic tissues and cell lines compared to the respective normal tissues. Moreover, the mouse model of skin carcinogenesis induced by the combined action of chemical carcinogens and phorbol esters was used to identify the stage of TB10 gene induction. The expression was almost undetectable in normal keratinocytes, its induction occurred even at the papilloma stage, however a further increased expression was observed in the carcinoma derived cell lines. Finally, immunohistochemical analysis of some breast, colon and ovary carcinoma samples by using specific anti-TB10 antibodies revealed the presence of the TB10 protein in all of the neoplastic tissues, but not in the respective normal tissues. Therefore the TB10 detection may be considered a potential tool for the diagnosis of several human neoplasias.

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