| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
From the Department of Physiopathology, Endocrinology
Unit*
and Department of Internal Medicine, Section of
Clinical Immunology, Allergy, and Respiratory
Diseases,
University of Florence, Florence,
Italy; and the Division of Clinical Immunology and
Allergy,
University of Naples Federico II,
Naples, Italy
Eosinophils, basophils, and Th2 cells express the chemokine receptor CCR3, which binds eotaxin, RANTES, and some other chemokines. Using immunohistochemistry and flow cytometry, we demonstrate that CCR3 is also expressed by a variable proportion of human mast cells in gut, skin, and lung tissue. By contrast, with the same anti-CCR3 antibody (B711), CCR3 was poorly if at all detectable on human Th2 cells in vitro and in vivo. Eotaxin neither induced histamine release from purified human mast cells nor increased anti-IgE-stimulated histamine secretion. However, both eotaxin and RANTES elicited mast cell migration in vitro with a similar efficacy. High percentages of CCR3-expressing mast cells were present in the skin and in the intestinal submucosa; much lower percentages were found in the intestinal mucosa and in lung interstitium. Double immunostaining with anti-CCR3 and anti-chymase antibody showed that the vast majority of CCR3-expressing mast cells in the various tissues examined were tryptase-chymase double-positive. Therefore, tryptase-chymase double-positive mast cells express CCR3 and are attracted by CCR3-binding chemokines, eotaxin, and RANTES. Our findings indicate that these chemokines may play an important role in the differentiation and/or migration of this mast cell subset in connective tissues, as well as in sites of allergic inflammation.
This article has been cited by other articles:
 |
|
 |
|
  E. L. Wise, C. Duchesnes, P. C. A. da Fonseca, R. A. Allen, T. J. Williams, and J. E. Pease Small Molecule Receptor Agonists and Antagonists of CCR3 Provide Insight into Mechanisms of Chemokine Receptor Activation J. Biol. Chem., September 21, 2007; 282(38): 27935 - 27943. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-P. Belot, L. Abdennebi-Najar, F. Gaudin, M. Lieberherr, V. Godot, J. Taieb, D. Emilie, and V. Machelon Progesterone reduces the migration of mast cells toward the chemokine stromal cell-derived factor-1/CXCL12 with an accompanying decrease in CXCR4 receptors Am J Physiol Endocrinol Metab, May 1, 2007; 292(5): E1410 - E1417. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Chen, P. Keshavan, G. D. Gregory, and D. J. Klumpp RANTES mediates TNF-dependent lamina propria mast cell accumulation and barrier dysfunction in neurogenic cystitis Am J Physiol Renal Physiol, May 1, 2007; 292(5): F1372 - F1379. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. de Paulis, N. Prevete, I. Fiorentino, F. W. Rossi, S. Staibano, N. Montuori, P. Ragno, A. Longobardi, B. Liccardo, A. Genovese, et al. Expression and Functions of the Vascular Endothelial Growth Factors and Their Receptors in Human Basophils J. Immunol., November 15, 2006; 177(10): 7322 - 7331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Madson, D. T. Lynch, K. L. Tinkum, S. K. Putta, and L. A. Hansen Erbb2 Regulates Inflammation and Proliferation in the Skin after Ultraviolet Irradiation Am. J. Pathol., October 1, 2006; 169(4): 1402 - 1414. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Woodman, A. Sutcliffe, D. Kaur, M. Berry, P. Bradding, I. D. Pavord, and C. E. Brightling Chemokine Concentrations and Mast Cell Chemotactic Activity in BAL Fluid in Patients With Eosinophilic Bronchitis and Asthma, and in Normal Control Subjects. Chest, August 1, 2006; 130(2): 371 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Sutcliffe, D Kaur, S Page, L Woodman, C L Armour, M Baraket, P Bradding, J M Hughes, and C E Brightling Mast cell migration to Th2 stimulated airway smooth muscle from asthmatics Thorax, August 1, 2006; 61(8): 657 - 662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Das, K. G. Vaddi, K. A. Solomon, C. Krauthauser, X. Jiang, K. W. McIntyre, X. X. Yang, E. Wadman, P. Welch, M. Covington, et al. Selective Inhibition of Eosinophil Influx into the Lung by Small Molecule CC Chemokine Receptor 3 Antagonists in Mouse Models of Allergic Inflammation J. Pharmacol. Exp. Ther., July 1, 2006; 318(1): 411 - 417. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pang, M. Nie, L. Corbett, A. Sutcliffe, and A. J. Knox Mast Cell beta-Tryptase Selectively Cleaves Eotaxin and RANTES and Abrogates Their Eosinophil Chemotactic Activities J. Immunol., March 15, 2006; 176(6): 3788 - 3795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Debes, M. E. Dahl, A. J. Mahiny, K. Bonhagen, D. J. Campbell, K. Siegmund, K. J. Erb, D. B. Lewis, T. Kamradt, and A. Hamann Chemotactic Responses of IL-4-, IL-10-, and IFN-{gamma}-Producing CD4+ T Cells Depend on Tissue Origin and Microbial Stimulus J. Immunol., January 1, 2006; 176(1): 557 - 566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Brightling, A. J. Ammit, D. Kaur, J. L. Black, A. J. Wardlaw, J. M. Hughes, and P. Bradding The CXCL10/CXCR3 Axis Mediates Human Lung Mast Cell Migration to Asthmatic Airway Smooth Muscle Am. J. Respir. Crit. Care Med., May 15, 2005; 171(10): 1103 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Brightling, D. Kaur, P. Berger, A. J. Morgan, A. J. Wardlaw, and P. Bradding Differential expression of CCR3 and CXCR3 by human lung and bone marrow-derived mast cells: implications for tissue mast cell migration J. Leukoc. Biol., May 1, 2005; 77(5): 759 - 766. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. de Paulis, N. Montuori, N. Prevete, I. Fiorentino, F. W. Rossi, V. Visconte, G. Rossi, G. Marone, and P. Ragno Urokinase Induces Basophil Chemotaxis through a Urokinase Receptor Epitope That Is an Endogenous Ligand for Formyl Peptide Receptor-Like 1 and -Like 2 J. Immunol., November 1, 2004; 173(9): 5739 - 5748. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. Kodali, W. J.H. Kim, I. I. Galaria, C. Miller, A. D. Schecter, S. A. Lira, and M. B. Taubman CCL11 (Eotaxin) Induces CCR3-Dependent Smooth Muscle Cell Migration Arterioscler. Thromb. Vasc. Biol., July 1, 2004; 24(7): 1211 - 1216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. de Paulis, N. Prevete, I. Fiorentino, A. F. Walls, M. Curto, A. Petraroli, V. Castaldo, P. Ceppa, R. Fiocca, and G. Marone Basophils Infiltrate Human Gastric Mucosa at Sites of Helicobacter pylori Infection, and Exhibit Chemotaxis in Response to H. pylori-derived Peptide Hp(2-20) J. Immunol., June 15, 2004; 172(12): 7734 - 7743. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F Qadri, T R Bhuiyan, K K Dutta, R Raqib, M S Alam, N H Alam, A-M Svennerholm, and M M Mathan Acute dehydrating disease caused by Vibrio cholerae serogroups O1 and O139 induce increases in innate cells and inflammatory mediators at the mucosal surface of the gut Gut, January 1, 2004; 53(1): 62 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-J. Jeong, H.-J. Na, S.-H. Hong, and H.-M. Kim Inhibition of the Stem Cell Factor-Induced Migration of Mast Cells by Dexamethasone Endocrinology, September 1, 2003; 144(9): 4080 - 4086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Warrior, E. M. Mckeegan, S. M. Rottinghaus, L. Garcia, L. Traphagen, G. Grayson, V. Komater, T. McNally, R. Helfrich, R. R. Harris, et al. Identification and Characterization of Novel Antagonists of the CCR3 Receptor J Biomol Screen, June 1, 2003; 8(3): 324 - 331. [Abstract] [PDF]
|
|
|
|
 |
|
 R. Raqib, P. K. Moly, P. Sarker, F. Qadri, N. H. Alam, M. Mathan, and J. Andersson Persistence of Mucosal Mast Cells and Eosinophils in Shigella-Infected Children Infect. Immun., May 1, 2003; 71(5): 2684 - 2692. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Forsythe and A. D. Befus CCR3: A Key to Mast Cell Phenotypic and Functional Diversity? Am. J. Respir. Cell Mol. Biol., April 1, 2003; 28(4): 405 - 409. [Full Text] [PDF]
|
|
|
|
|
|
K. S. Price, D. S. Friend, E. A. Mellor, N. De Jesus, G. F. M. Watts, and J. A. Boyce CC Chemokine Receptor 3 Mobilizes to the Surface of Human Mast Cells and Potentiates Immunoglobulin E-Dependent Generation of Interleukin 13 Am. J. Respir. Cell Mol. Biol., April 1, 2003; 28(4): 420 - 427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Genovese, G. Borgia, L. Bjorck, A. Petraroli, A. de Paulis, M. Piazza, and G. Marone Immunoglobulin Superantigen Protein L Induces IL-4 and IL-13 Secretion from Human Fc{varepsilon}RI+ Cells Through Interaction with the {kappa} Light Chains of IgE J. Immunol., February 15, 2003; 170(4): 1854 - 1861. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. de Paulis, G. Florio, N. Prevete, M. Triggiani, I. Fiorentino, A. Genovese, and G. Marone HIV-1 Envelope gp41 Peptides Promote Migration of Human Fc{epsilon}RI+ Cells and Inhibit IL-13 Synthesis Through Interaction with Formyl Peptide Receptors J. Immunol., October 15, 2002; 169(8): 4559 - 4567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. F. Gurish, A. Humbles, H. Tao, S. Finkelstein, J. A. Boyce, C. Gerard, D. S. Friend, and K. F. Austen CCR3 Is Required for Tissue Eosinophilia and Larval Cytotoxicity After Infection with Trichinella spiralis J. Immunol., June 1, 2002; 168(11): 5730 - 5736. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. S. Cheng, N. W. Lukacs, and S. L. Kunkel Eotaxin/CCL11 Suppresses IL-8/CXCL8 Secretion from Human Dermal Microvascular Endothelial Cells J. Immunol., March 15, 2002; 168(6): 2887 - 2894. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. D'AMBROSIO, M. MARIANI, P. PANINA-BORDIGNON, and F. SINIGAGLIA Chemokines and Their Receptors Guiding T Lymphocyte Recruitment in Lung Inflammation Am. J. Respir. Crit. Care Med., October 1, 2001; 164(7): 1266 - 1275. [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, L. Li, R. Wadley, S. W. Reddel, J. C. Qi, C. Archis, A. Collins, E. Clark, M. Cooley, S. Kouts, et al. Mast cells/basophils in the peripheral blood of allergic individuals who are HIV-1 susceptible due to their surface expression of CD4 and the chemokine receptors CCR3, CCR5, and CXCR4 Blood, June 1, 2001; 97(11): 3484 - 3490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Kambe, M. Kambe, J. P. Kochan, and L. B. Schwartz Human skin-derived mast cells can proliferate while retaining their characteristic functional and protease phenotypes Blood, April 1, 2001; 97(7): 2045 - 2052. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Stellato, M. E. Brummet, J. R. Plitt, S. Shahabuddin, F. M. Baroody, M. C. Liu, P. D. Ponath, and L. A. Beck Cutting Edge: Expression of the C-C Chemokine Receptor CCR3 in Human Airway Epithelial Cells J. Immunol., February 1, 2001; 166(3): 1457 - 1461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. de Paulis, R. De Palma, L. Di Gioia, M. Carfora, N. Prevete, G. Tosi, R. S. Accolla, and G. Marone Tat Protein Is an HIV-1-Encoded {beta}-Chemokine Homolog That Promotes Migration and Up-Regulates CCR3 Expression on Human Fc{epsilon}RI+ Cells J. Immunol., December 15, 2000; 165(12): 7171 - 7179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. H. Falcone, H. Haas, and B. F. Gibbs The human basophil: a new appreciation of its role in immune responses Blood, December 15, 2000; 96(13): 4028 - 4038. [Full Text] [PDF]
|
|
|
|
|
|
F. J. Culley, A. Brown, D. M. Conroy, I. Sabroe, D. I. Pritchard, and T. J. Williams Eotaxin Is Specifically Cleaved by Hookworm Metalloproteases Preventing Its Action In Vitro and In Vivo J. Immunol., December 1, 2000; 165(11): 6447 - 6453. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Genovese, J.-P. Bouvet, G. Florio, B. Lamparter-Schummert, L. Bjorck, and G. Marone Bacterial Immunoglobulin Superantigen Proteins A and L Activate Human Heart Mast Cells by Interacting with Immunoglobulin E Infect. Immun., October 1, 2000; 68(10): 5517 - 5524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Zimmermann, B. L. Daugherty, J. L. Kavanaugh, F. Y. El-Awar, E. A. Moulton, and M. E. Rothenberg Analysis of the CC chemokine receptor 3 gene reveals a complex 5' exon organization, a functional role for untranslated exon 1, and a broadly active promoter with eosinophil-selective elements Blood, October 1, 2000; 96(7): 2346 - 2354. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
