| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
From the Division of Comparative Pathology, New England Regional Primate Research Center, Harvard Medical School, Southborough, Massachusetts
Neonatal human immunodeficiency virus (HIV) infection usually occurs intrapartum or postpartum and results in a higher incidence of neurological dysfunction than is seen in adults. To explore the neuropathogenesis of neonatal HIV infection, we infected neonatal macaques with simian immunodeficiency virus (SIV) and followed the course of infection focusing on early time points. Infected neonates had decreased brain growth and mild histological changes in brain that resembled those seen in pediatric AIDS, including perivascular infiltrates of mononuclear cells, mineralization of vessels in the basal ganglia, and gliosis. The perivascular lesions and gliosis were associated with the presence of occasional infected cells that required in situ hybridization with radiolabeled riboprobes for detection. Using this technique, SIV-infected cells were detected in the brain parenchyma within 7 days of infection. These findings were confirmed by nested PCR for SIVgag DNA in brain and RT-PCR for viral RNA in cerebrospinal fluid. Together, these techniques revealed SIV infection of the CNS in 12 of 13 neonates infected with SIVmac239, 3 of 3 infected with SIVmac251, and 2 of 2 infected with SIVmac239/316. The prevalence of CNS infection was indistinguishable from that of older animals infected with the same dose and stock of virus, but neonates appeared to have fewer infected cells in the CNS and detecting them required more sensitive techniques. This observation was true regardless of inoculum and despite the fact that neonates had equal or greater viral loads in the periphery compared with older animals. These data suggest that maturation-dependent host factors have a major impact on the neuropathogenesis of pediatric AIDS.
This article has been cited by other articles:
 |
|
 |
|
  M. S. Orandle, R. S. Veazey, and A. A. Lackner Enteric Ganglionitis in Rhesus Macaques Infected with Simian Immunodeficiency Virus J. Virol., June 15, 2007; 81(12): 6265 - 6275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Borda, X. Alvarez, I. Kondova, P. Aye, M. A. Simon, R. C. Desrosiers, and A. A. Lackner Cell Tropism of Simian Immunodeficiency Virus in Culture Is Not Predictive of in Vivo Tropism or Pathogenesis Am. J. Pathol., December 1, 2004; 165(6): 2111 - 2122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. S. Veazey, J. D. Lifson, I. Pandrea, J. Purcell, M. Piatak Jr., and A. A. Lackner Simian Immunodeficiency Virus Infection in Neonatal Macaques J. Virol., August 15, 2003; 77(16): 8783 - 8792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Johnston, C. Silva, and C. Power Envelope Gene-Mediated Neurovirulence in Feline Immunodeficiency Virus Infection: Induction of Matrix Metalloproteinases and Neuronal Injury J. Virol., March 15, 2002; 76(6): 2622 - 2633. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Orandle, K. C. Williams, A. G. MacLean, S. V. Westmoreland, and A. A. Lackner Macaques with Rapid Disease Progression and Simian Immunodeficiency Virus Encephalitis Have a Unique Cytokine Profile in Peripheral Lymphoid Tissues J. Virol., May 1, 2001; 75(9): 4448 - 4452. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Alexander, L. Denekamp, S. Czajak, and R. C. Desrosiers Suboptimal Nucleotides in the Infectious, Pathogenic Simian Immunodeficiency Virus Clone SIVmac239 J. Virol., April 15, 2001; 75(8): 4019 - 4022. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
