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(American Journal of Pathology. 1999;155:1439-1443.)
© 1999 American Society for Investigative Pathology

Short Communications

MYCN Gene Amplification

Identification of Cell Populations Containing Double Minutes andHomogeneously Staining Regions in Neuroblastoma Tumors

Maisa Yoshimoto^*, Silvia Regina Caminada de Toledo^*, Eliana Maria Monteiro Caran^§, Maria Teresa de Seixas, Maria Lucia de Martino Lee^§, Simone de Campos Vieira Abib, Sonia Maria Rossi Vianna^¶, Sergio Thomaz Schettini and Joyce Anderson Duffles Andrade^*

From the Division of Genetics of the Department of Morphology,^*
the Division of Medical Pathology of the Department of Pathology,
and the Division of Pediatric Surgery of the Department of Surgery,
Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo; the Instituto de Oncologia Pediátrica,^§
Universidade Federal de São Paulo, Escola Paulista de Medicina, São Paulo; and the Division of Pediatric Oncology,^¶
Hospital Servidor Público Estadual, São Paulo, Brazil

Neuroblastoma is the second most common solid tumor occurring in children. Amplification of the MYCN oncogene is associated with poor prognosis. To identify neuroblastoma tumors with MYCN amplification, we studied the number of copies of MYCN in interphase cells by fluorescence in situ hybridization in 20 neuroblastoma patients. MYCN amplification appeared in 7 tumor specimens. Interphase and metaphase studies showed a tumor cell population with both forms of amplification, double minutes and homogeneously staining regions, in two patients. These patients showed a smaller tumor cell subpopulation with the presence of more than one homogeneously staining region, suggesting that gene amplification was undergoing karyotype evolution.

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