help button home button Am J Pathol R & D Systems
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Katou, F.
Right arrow Articles by Motegi, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Katou, F.
Right arrow Articles by Motegi, K.
(American Journal of Pathology. 2000;156:519-527.)
© 2000 American Society for Investigative Pathology

Regular Articles

Immunological Activation of Dermal Langerhans Cells in Contact with Lymphocytes in a Model of Human Inflamed Skin

Fuminori Katou*, Haruo Ohtani{dagger}, Anne Saaristo{ddagger}, Hiroshi Nagura{dagger} and Katsutoshi Motegi*

From the Departments of Oral and Maxillofacial Surgery I*
and Pathology,{dagger}
Tohoku University, Sendai, Japan; and the Molecular/Cancer Biology Laboratory and Department of Pathology,{ddagger}
Haartman Institute, University of Helsinki, Helsinki, Finland

Langerhans cells play an important role in the skin’s immune system. Little is known, however, about the antigen-presenting capacity of Langerhans cells in the context of skin inflammation. By immunohistochemistry we investigated the phenotypic characteristics of epidermal and dermal Langerhans cells and their spatial relationship with infiltrating lymphocytes. We studied skin flaps autotransplanted to the oral cavity to fill a defect after maxillofacial cancer surgery. In 15 of 21 cases sampled for the present study, the skin flaps were severely inflamed by Candida albicans infection. In contrast to the normal skin, such inflamed skin showed a marked increase in CD1a+ dermal Langerhans cells. Double immunohistochemistry revealed that dermal Langerhans cells abundantly expressed B7–2 (CD86), a representative costimulatory molecule, and CD83, a marker of mature dendritic cells. Furthermore, these dermal Langerhans cells were in close contact with CD4+/CD45RO+ lymphocytes. This cell-to-cell contact was further visualized by immunoelectron microscopy. Langerhans cells were also observed within lymphatic vessels that were identified by the expression of vascular endothelial growth factor receptor-3. Ki-67 labeling indices were 4.2% in CD4+ T cells and 0.8% in CD8+ T cells within the dermis. Factor XIIIa+ dermal dendrocytes were distributed outside the clusters of lymphocytes and were not in contact with them. Our observations indicate that dermal Langerhans cells in the inflamed skin are activated to express common phenotypes to mature dendritic cells so that they could stimulate neighboring memory CD4+ T cells.




This article has been cited by other articles:


Home page
 J. Dent. Res.Home page
C.W. Cutler and R. Jotwani
Dendritic cells at the oral mucosal interface.
J. Dent. Res., August 1, 2006; 85(8): 678 - 689.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. E. Angel, E. George, A. E. S. Brooks, L. L. Ostrovsky, T. L. H. Brown, and P. R. Dunbar
Cutting Edge: CD1a+ Antigen-Presenting Cells in Human Dermis Respond Rapidly to CCR7 Ligands
J. Immunol., May 15, 2006; 176(10): 5730 - 5734.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
C. Gunther, C. Bello-Fernandez, T. Kopp, J. Kund, N. Carballido-Perrig, S. Hinteregger, S. Fassl, C. Schwarzler, G. Lametschwandtner, G. Stingl, et al.
CCL18 Is Expressed in Atopic Dermatitis and Mediates Skin Homing of Human Memory T Cells
J. Immunol., February 1, 2005; 174(3): 1723 - 1728.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Microbiol. Rev.Home page
L. de Repentigny, D. Lewandowski, and P. Jolicoeur
Immunopathogenesis of Oropharyngeal Candidiasis in Human Immunodeficiency Virus Infection
Clin. Microbiol. Rev., October 1, 2004; 17(4): 729 - 759.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
M. Anlauf, M. K.-H. Schafer, C. Depboylu, W. Hartschuh, L. E. Eiden, G. Kloppel, and E. Weihe
The Vesicular Monoamine Transporter 2 (VMAT2) Is Expressed by Normal and Tumor Cutaneous Mast Cells and Langerhans Cells of the Skin but Is Absent from Langerhans Cell Histiocytosis
J. Histochem. Cytochem., June 1, 2004; 52(6): 779 - 788.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
R. Jotwani and C.W. Cutler
Multiple Dendritic Cell (DC) Subpopulations in Human Gingiva and Association of Mature DCs with CD4+ T-cells in situ
J. Dent. Res., September 1, 2003; 82(9): 736 - 741.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
W. Iijima, H. Ohtani, T. Nakayama, Y. Sugawara, E. Sato, H. Nagura, O. Yoshie, and T. Sasano
Infiltrating CD8+ T Cells in Oral Lichen Planus Predominantly Express CCR5 and CXCR3 and Carry Respective Chemokine Ligands RANTES/CCL5 and IP-10/CXCL10 in Their Cytolytic Granules: A Potential Self-Recruiting Mechanism
Am. J. Pathol., July 1, 2003; 163(1): 261 - 268.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F. Katou, H. Ohtani, T. Nakayama, K. Ono, K. Matsushima, A. Saaristo, H. Nagura, O. Yoshie, and K. Motegi
Macrophage-Derived Chemokine (MDC/CCL22) and CCR4 Are Involved in the Formation of T Lymphocyte-Dendritic Cell Clusters in Human Inflamed Skin and Secondary Lymphoid Tissue
Am. J. Pathol., April 1, 2001; 158(4): 1263 - 1270.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Society for Investigative Pathology.