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(American Journal of Pathology. 2000;156:807-812.)
© 2000 American Society for Investigative Pathology

Short Communications

API2-MALT1 Chimeric Transcripts Involved in Mucosa-Associated Lymphoid Tissue Type Lymphoma Predict Heterogeneous Products

Mutsuhito Motegi^*, Masakatsu Yonezumi^*, Hiroko Suzuki^*, Ritsuro Suzuki^*, Yoshitaka Hosokawa^*, Shigetoshi Hosaka^§, Yoshihisa Kodera^¶, Yasuo Morishima, Shigeo Nakamura and Masao Seto^*

From the Laboratory of Chemotherapy, Aichi Cancer Center Research Institute,^*
the Department of Hematology and Chemotherapy,
and the Department of Pathology and Clinical Laboratories,
Aichi Cancer Center Hospital, Nagoya; the Second Department of Internal Medicine,^§
Shinshu University School of Medicine, Nagano; and the Department of Hematology,^¶
Japanese Red Cross Nagoya First Hospital, Nagoya, Japan

Recent progress in molecular analysis of low-grade B cell lymphoma has revealed that API2 at 11q21 and a novel gene, MALT1 at 18q21, are involved in t(11;18)(q21;q21), a characteristic chromosome aberration for mucosa-associated lymphoid tissue (MALT) type lymphoma. We describe here the establishment of a reverse transcription-polymerase chain reaction (RT-PCR) assay that we used to analyze 22 cases of MALT lymphoma. All five cases that were shown to possess t(11;18)(q21;q21) showed the specific amplification of API2-MALT1 chimeric transcripts. Of the remaining 17 cases for which cytogenetic data were not available, three cases demonstrated the presence of fusion transcripts, indicating that a significant percentage of MALT lymphoma cases of the present series appeared to possess t(11;18). A single fragment was observed in each of these cases, but the size varied from case to case. Sequencing analysis revealed that there are two breakpoints in API2 and three in MALT1, and that all of the fusion transcripts are in-frame. On the basis of these results, four kinds of chimeric proteins can be predicted for the present series. Thus, the RT-PCR assay used here should serve as an effective molecular tool for understanding molecular pathogenesis and the clinical significance of API2-MALT1 for MALT lymphomas.

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