| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Short Communications |
From the Department of Anatomy and Histology and Institute of
Biomedical Research*
and the Department of
Medicine,
University of Sydney, Sydney, New
South Wales, Australia
The cellular and microvascular responses of JC Lewis rats to an
intravenous injection of activated T cells specific for ovalbumin were
examined with the retinal whole mount technique. The retina was
examined at various times post-injection (pi) with the use of
antibodies to the 
ß T cell receptor (TCR) or to major
histocompatibility complex class II (MHC II), the monoclonal
antibody ED1, and intravascular tracers. By 12 hours
pi, small numbers of TCR+,
ED1+, and MHC II+ cells were present
within the lumen of retinal vessels, and minor breakdown of the
blood-retinal barrier (BRB) and microglial activation were evident. The
intensity of these responses had increased by 1 day pi, when
small numbers of TCR+ cells had also undergone
extravasation. By 2 to 3 days pi, the numbers of
TCR+, ED1+, and MHC
II+ cells in the retinal parenchyma had increased,
but the BRB breakdown and microglial activation had subsided.
Thus, in the absence of target antigen, activated T
cells induced limited and transient breakdown of the BRB,
microglial activation, and the extravasation of
ED1+, MHC II+ monocytes. In
contrast, the retina of rats that received an intraocular
injection of ovalbumin in addition to the intravascular injection of T
cells showed massive cellular recruitment and breakdown of the BRB.
These results indicate that an increase in the number of activated T
cells in the circulation, such as that which occurs during
viral or bacterial infection, has the potential to result in
transient breakdown of the BRB and a mild local microglial
response.
This article has been cited by other articles:
 |
|
 |
|
  D. S. Gregerson, N. D. Heuss, U. Lehmann, and S. W. McPherson Peripheral Induction of Tolerance by Retinal Antigen Expression J. Immunol., July 15, 2009; 183(2): 814 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Jiang, Y. Ke, D. Sun, G. Han, H. J. Kaplan, and H. Shao Reactivation of Uveitogenic T Cells by Retinal Astrocytes Derived from Experimental Autoimmune Uveitis-Prone B10RIII Mice Invest. Ophthalmol. Vis. Sci., January 1, 2008; 49(1): 282 - 289. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xu, R. Dawson, J. V. Forrester, and J. Liversidge Identification of Novel Dendritic Cell Populations in Normal Mouse Retina Invest. Ophthalmol. Vis. Sci., April 1, 2007; 48(4): 1701 - 1710. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Gregerson and H. Kawashima APC derived from donor splenocytes support retinal autoimmune disease in allogeneic recipients J. Leukoc. Biol., August 1, 2004; 76(2): 383 - 387. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Gregerson, T. N. Sam, and S. W. McPherson The Antigen-Presenting Activity of Fresh, Adult Parenchymal Microglia and Perivascular Cells from Retina J. Immunol., June 1, 2004; 172(11): 6587 - 6597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Nitta, M. Hata, S. Gotoh, Y. Seo, H. Sasaki, N. Hashimoto, M. Furuse, and S. Tsukita Size-selective loosening of the blood-brain barrier in claudin-5-deficient mice J. Cell Biol., May 12, 2003; 161(3): 653 - 660. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Xu, J. V. Forrester, J. Liversidge, and I. J. Crane Leukocyte Trafficking in Experimental Autoimmune Uveitis: Breakdown of Blood-Retinal Barrier and Upregulation of Cellular Adhesion Molecules Invest. Ophthalmol. Vis. Sci., January 1, 2003; 44(1): 226 - 234. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Kim, M. Zhang, B. P. Vistica, C.-C. Chan, D.-F. Shen, E. F. Wawrousek, and I. Gery Induction of Ocular Inflammation by T-Helper Lymphocytes Type 2 Invest. Ophthalmol. Vis. Sci., March 1, 2002; 43(3): 758 - 765. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
