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(American Journal of Pathology. 2000;156:1227-1234.)
© 2000 American Society for Investigative Pathology

Regular Articles

Detection of TT Virus DNA in Liver Biopsies by in Situ Hybridization

Elena Rodríguez-Iñigo*{dagger}, Mercedes Casqueiro*{dagger}, Javier Bartolomé*{dagger}, Nuria Ortiz-Movilla*{dagger}, Juan Manuel López-Alcorocho*{dagger}, Montserrat Herrero*{dagger}, Félix Manzarbeitia{ddagger}, Horacio Oliva{ddagger} and Vicente Carreño*{dagger}

From the Departments of Hepatology*
and Pathology,{ddagger}
Fundación Jiménez Díaz, and the Fundación Estudio Hepatitis Virales,{dagger}
Madrid, Spain

A novel hepatitis-associated virus named TT virus (TTV) has been isolated. However, its hepatotropism has not been proven. We have retrospectively analyzed the presence of TTV-DNA by polymerase chain reaction (PCR) and in situ hybridization in liver biopsies from 30 patients with liver disease (15 TTV-DNA-positive and 15 TTV-DNA-negative in serum), and prospectively in serum and liver from eight patients with normal liver histology. TTV-DNA was detected by PCR in the liver from the 15 patients with serum TTV-DNA and in serum and liver of two of the eight patients without liver disease. TTV-DNA titers in liver were 10 times higher than in serum, although no correlation between TTV-DNA titers in serum and liver were observed. In situ hybridization shows positive signals in the hepatocytes of the 17 patients infected by TTV but in none of the TTV-DNA-negative patients by PCR. No morphological changes were observed in the hepatocytes showing hybridization signals. The percentage of positive hepatocytes ranged from 2.1% to 30% and correlated with the TTV-DNA titers in liver (r = 0.54; P = 0.037). In conclusion, our results show that TTV is able to infect liver cells although they do not support a role for TTV in causing liver disease.




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 Clin. Microbiol. Rev.Home page
M. Bendinelli, M. Pistello, F. Maggi, C. Fornai, G. Freer, and M. L. Vatteroni
Molecular Properties, Biology, and Clinical Implications of TT Virus, a Recently Identified Widespread Infectious Agent of Humans
Clin. Microbiol. Rev., January 1, 2001; 14(1): 98 - 113.
[Abstract] [Full Text] [PDF]


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