help button home button Am J Pathol ASIP MEMBERSHIP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baens, M.
Right arrow Articles by De Wolf-Peeters, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baens, M.
Right arrow Articles by De Wolf-Peeters, C.
(American Journal of Pathology. 2000;156:1433-1439.)
© 2000 American Society for Investigative Pathology

Regular Articles

The Product of the t(11;18), an API2-MLT Fusion, Marks Nearly Half of Gastric MALT Type Lymphomas without Large Cell Proliferation

Mathijs Baens*, Brigitte Maes{dagger}, Anja Steyls*, Karel Geboes{dagger}, Peter Marynen* and Christiane De Wolf-Peeters{dagger}

From the Human Genome Laboratory,*
Center for Human Genetics, Flanders Interuniversity Institute for Biotechnology, Leuven; and the Department of Pathology,{dagger}
University of Leuven, Leuven, Belgium

Recently we demonstrated that the t(11;18)(q21;q21) associated with extranodal marginal zone B cell lymphomas of MALT type results in the expression of a chimeric transcript fusing 5' API2 on chromosome 11 to 3' MLT on chromosome 18. Here we report the development of an RT-PCR approach for the detection of the API2-MLT fusion transcript and its application for the analysis of 58 cases of gastric lymphoma. Initially nested PCR amplification was combined with Southern analysis using internal API2 and MLT probes. A genuine API2-MLT fusion transcript of variable length was demonstrated in 11 out of 58 cases. Sequence analysis revealed that in all cases the breakpoint on chromosome 11 occurred between exons 7 and 8 of the API2 gene. In contrast, the breakpoints on chromosome 18 appeared to be heterogeneous as fusions to bp 814, 1123, and 1150, respectively, of MLT were observed. These observations allowed us to work out a highly sensitive diagnostic test for the API2-MLT fusion on an ABI Prism 7700 sequence detector that confirmed the results of our initial approach. The API2-MLT fusion was found in 48% of gastric marginal zone cell lymphomas of MALT type that did not contain a large cell component and it was lacking in all other lymphomas of the stomach.




This article has been cited by other articles:


Home page
 haematolHome page
E. M. Murga Penas, E. Callet-Bauchu, H. Ye, K. Hinz, N. Albert, C. Copie-Bergman, S. Gazzo, F. Berger, G. Salles, C. Bokemeyer, et al.
The translocations t(6;18;11)(q24;q21;q21) and t(11;14;18)(q21;q32;q21) lead to a fusion of the API2 and MALT1 genes and occur in MALT lymphomas
Haematologica, March 1, 2007; 92(3): 405 - 409.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
M-Q Du and J C Atherton
Molecular subtyping of gastric MALT lymphomas: implications for prognosis and management.
Gut, June 1, 2006; 55(6): 886 - 893.
[Full Text] [PDF]

Home page
 JCOHome page
X. Sagaert, P. de Paepe, L. Libbrecht, V. Vanhentenrijk, G. Verhoef, J. Thomas, I. Wlodarska, and C. De Wolf-Peeters
Forkhead Box Protein P1 Expression in Mucosa-Associated Lymphoid Tissue Lymphomas Predicts Poor Prognosis and Transformation to Diffuse Large B-Cell Lymphoma
J. Clin. Oncol., June 1, 2006; 24(16): 2490 - 2497.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
K Volp, M-L Brezniceanu, S Bosser, T Brabletz, T Kirchner, D Gottel, S Joos, and M Zornig
Increased expression of high mobility group box 1 (HMGB1) is associated with an elevated level of the antiapoptotic c-IAP2 protein in human colon carcinomas
Gut, February 1, 2006; 55(2): 234 - 242.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
T. Wundisch, C. Thiede, A. Morgner, A. Dempfle, A. Gunther, H. Liu, H. Ye, M.-Q. Du, T. D. Kim, E. Bayerdorffer, et al.
Long-Term Follow-Up of Gastric MALT Lymphoma After Helicobacter Pylori Eradication
J. Clin. Oncol., November 1, 2005; 23(31): 8018 - 8024.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
A. Salar, B. Bellosillo, S. Serrano, and C. Besses
Persistent Residual Disease in t(11;18)(q21;q21) Positive Gastric Mucosa-Associated Lymphoid Tissue Lymphoma Treated With Chemotherapy or Rituximab
J. Clin. Oncol., October 10, 2005; 23(29): 7361 - 7362.
[Full Text] [PDF]

Home page
 JNCI J Natl Cancer InstHome page
L.-T. Chen, J.-T. Lin, J. J. Tai, G.-H. Chen, H.-Z. Yeh, S.-S. Yang, H.-P. Wang, S.-H. Kuo, B.-S. Sheu, C.-M. Jan, et al.
Long-Term Results of Anti-Helicobacter pylori Therapy in Early-Stage Gastric High-Grade Transformed MALT Lymphoma
J Natl Cancer Inst, September 21, 2005; 97(18): 1345 - 1353.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
P. Farinha and R. D. Gascoyne
Molecular Pathogenesis of Mucosa-Associated Lymphoid Tissue Lymphoma
J. Clin. Oncol., September 10, 2005; 23(26): 6370 - 6378.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
F. Bertoni and E. Zucca
State-of-the-Art Therapeutics: Marginal-Zone Lymphoma
J. Clin. Oncol., September 10, 2005; 23(26): 6415 - 6420.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
M. Levy, C. Copie-Bergman, C. Gameiro, M.-T. Chaumette, M.-H. Delfau-Larue, C. Haioun, A. Charachon, F. Hemery, P. Gaulard, K. Leroy, et al.
Prognostic Value of Translocation t(11;18) in Tumoral Response of Low-Grade Gastric Lymphoma of Mucosa-Associated Lymphoid Tissue Type to Oral Chemotherapy
J. Clin. Oncol., August 1, 2005; 23(22): 5061 - 5066.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K.-H. Yeh, S.-H. Kuo, L.-T. Chen, T.-L. Mao, S.-L. Doong, M.-S. Wu, H.-C. Hsu, Y.-S. Tzeng, C.-L. Chen, J.-T. Lin, et al.
Nuclear expression of BCL10 or nuclear factor kappa B helps predict Helicobacter pylori-independent status of low-grade gastric mucosa-associated lymphoid tissue lymphomas with or without t(11;18)(q21;q21)
Blood, August 1, 2005; 106(3): 1037 - 1041.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
U. Fischer and K. Schulze-Osthoff
New Approaches and Therapeutics Targeting Apoptosis in Disease
Pharmacol. Rev., June 1, 2005; 57(2): 187 - 215.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
S.-H. Kuo, L.-T. Chen, K.-H. Yeh, M.-S. Wu, H.-C. Hsu, P.-Y. Yeh, T.-L. Mao, C.-L. Chen, S.-L. Doong, J.-T. Lin, et al.
Nuclear Expression of BCL10 or Nuclear Factor Kappa B Predicts Helicobacter pylori-Independent Status of Early-Stage, High-Grade Gastric Mucosa-Associated Lymphoid Tissue Lymphomas
J. Clin. Oncol., September 1, 2004; 22(17): 3491 - 3497.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. Wohlfart, D. Sebinger, P. Gruber, J. Buch, D. Polgar, G. Krupitza, M. Rosner, M. Hengstschlager, M. Raderer, A. Chott, et al.
FAS (CD95) Mutations Are Rare in Gastric MALT Lymphoma but Occur More Frequently in Primary Gastric Diffuse Large B-Cell Lymphoma
Am. J. Pathol., March 1, 2004; 164(3): 1081 - 1089.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
B. Streubel, D. Huber, S. Wohrer, A. Chott, and M. Raderer
Frequency of Chromosomal Aberrations Involving MALT1 in Mucosa-Associated Lymphoid Tissue Lymphoma in Patients with Sjogren's Syndrome
Clin. Cancer Res., January 15, 2004; 10(2): 476 - 480.
[Abstract] [Full Text] [PDF]

Home page
 Ann OncolHome page
S. A. Pileri, P. L. Zinzani, P. Went, A. Pileri Jr, and M. Bendandi
Indolent lymphoma: the pathologist's viewpoint
Ann. Onc., January 1, 2004; 15(1): 12 - 18.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
R. W. Tsang, M. K. Gospodarowicz, M. Pintilie, W. Wells, D. C. Hodgson, A. Sun, M. Crump, and B. J. Patterson
Localized Mucosa-Associated Lymphoid Tissue Lymphoma Treated With Radiation Therapy Has Excellent Clinical Outcome
J. Clin. Oncol., November 15, 2003; 21(22): 4157 - 4164.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Ye, H. Liu, A. Attygalle, A. C. Wotherspoon, A. G. Nicholson, F. Charlotte, V. Leblond, P. Speight, J. Goodlad, A. Lavergne-Slove, et al.
Variable frequencies of t(11;18)(q21;q21) in MALT lymphomas of different sites: significant association with CagA strains of H pylori in gastric MALT lymphoma
Blood, August 1, 2003; 102(3): 1012 - 1018.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
Y Kaneko, S Sakurai, M Hironaka, S Sato, S Oguni, Y Sakuma, K Sato, K Sugano, and K Saito
Distinct methylated profiles in Helicobacter pylori dependent and independent gastric MALT lymphomas
Gut, May 1, 2003; 52(5): 641 - 646.
[Abstract] [Full Text]

Home page
 Am. J. Pathol.Home page
M. Okabe, H. Inagaki, K. Ohshima, T. Yoshino, C. Li, T. Eimoto, R. Ueda, and S. Nakamura
API2-MALT1 Fusion Defines a Distinctive Clinicopathologic Subtype in Pulmonary Extranodal Marginal Zone B-Cell Lymphoma of Mucosa-Associated Lymphoid Tissue
Am. J. Pathol., April 1, 2003; 162(4): 1113 - 1122.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Ye, H. Liu, M. Raderer, A. Chott, A. Ruskone-Fourmestraux, A. Wotherspoon, M. J. S. Dyer, S.-S. Chuang, A. Dogan, P. G. Isaacson, et al.
High incidence of t(11;18)(q21;q21) in Helicobacter pylori-negative gastric MALT lymphoma
Blood, April 1, 2003; 101(7): 2547 - 2550.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. Streubel, A. Lamprecht, J. Dierlamm, L. Cerroni, M. Stolte, G. Ott, M. Raderer, and A. Chott
T(14;18)(q32;q21) involving IGH and MALT1 is a frequent chromosomal aberration in MALT lymphoma
Blood, March 15, 2003; 101(6): 2335 - 2339.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
M. Sanchez-Beato, A. Sanchez-Aguilera, and M. A. Piris
Cell cycle deregulation in B-cell lymphomas
Blood, February 15, 2003; 101(4): 1220 - 1235.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
S Nakamura, T Matsumoto, S Nakamura, Y Jo, K Fujisawa, H Suekane, T Yao, M Tsuneyoshi, and M Iida
Chromosomal translocation t(11;18)(q21;q21) in gastrointestinal mucosa associated lymphoid tissue lymphoma
J. Clin. Pathol., January 1, 2003; 56(1): 36 - 42.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. F. E. Barth, M. Bentz, F. Leithauser, S. Stilgenbauer, R. Siebert, M. Schlotter, R. F. Schlenk, H. Dohner, P. Moller, P. Starostik, et al.
Pathogenic complexity of gastric B-cell lymphoma
Blood, August 1, 2002; 100(3): 1095 - 1097.
[Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
E. D. Remstein, P. J. Kurtin, C. D. James, X.-Y. Wang, R. G. Meyer, and G. W. Dewald
Mucosa-Associated Lymphoid Tissue Lymphomas with t(11;18)(q21;q21) and Mucosa-Associated Lymphoid Tissue Lymphomas with Aneuploidy Develop Along Different Pathogenetic Pathways
Am. J. Pathol., July 1, 2002; 161(1): 63 - 71.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. Inagaki, J. K. C. Chan, J. W. M. Ng, M. Okabe, T. Yoshino, M. Okamoto, H. Ogawa, H. Matsushita, T. Yokose, Y. Matsuno, et al.
Primary Thymic Extranodal Marginal-Zone B-Cell Lymphoma of Mucosa-Associated Lymphoid Tissue Type Exhibits Distinctive Clinicopathological and Molecular Features
Am. J. Pathol., April 1, 2002; 160(4): 1435 - 1443.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
B. Maes, A. Demunter, B. Peeters, and C. De Wolf-Peeters
BCL10 mutation does not represent an important pathogenic mechanism in gastric MALT-type lymphoma, and the presence of the API2-MLT fusion is associated with aberrant nuclear BCL10 expression
Blood, February 15, 2002; 99(4): 1398 - 1404.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
P. Starostik, J. Patzner, A. Greiner, S. Schwarz, J. Kalla, G. Ott, and H. K. Muller-Hermelink
Gastric marginal zone B-cell lymphomas of MALT type develop along 2 distinct pathogenetic pathways
Blood, January 1, 2002; 99(1): 3 - 9.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
Y. Hara, N. Nakamura, T. Kuze, Y. Hashimoto, Y. Sasaki, A. Shirakawa, M. Furuta, K. Yago, K. Kato, and M. Abe
Immunoglobulin Heavy Chain Gene Analysis of Ocular Adnexal Extranodal Marginal Zone B-Cell Lymphoma
Invest. Ophthalmol. Vis. Sci., October 1, 2001; 42(11): 2450 - 2457.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
H. Liu, H. Ye, A. Dogan, R. Ranaldi, R. A. Hamoudi, I. Bearzi, P. G. Isaacson, and M.-Q. Du
T(11;18)(q21;q21) is associated with advanced mucosa-associated lymphoid tissue lymphoma that expresses nuclear BCL10
Blood, August 15, 2001; 98(4): 1182 - 1187.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
H. Inagaki, M. Okabe, M. Seto, S. Nakamura, R. Ueda, and T. Eimoto
API2-MALT1 Fusion Transcripts Involved in Mucosa-Associated Lymphoid Tissue Lymphoma : Multiplex RT-PCR Detection Using Formalin-Fixed Paraffin-Embedded Specimens
Am. J. Pathol., February 1, 2001; 158(2): 699 - 706.
[Abstract] [Full Text] [PDF]

Home page
 ASH Education BookHome page
F. Cavalli, P. G. Isaacson, R. D. Gascoyne, and E. Zucca
MALT Lymphomas
Hematology, January 1, 2001; 2001(1): 241 - 258.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
D. A. Arber
Molecular Diagnostic Approach to Non-Hodgkin's Lymphoma
J. Mol. Diagn., November 1, 2000; 2(4): 178 - 190.
[Full Text]

Home page
 BloodHome page
J. Dierlamm, M. Baens, M. Stefanova-Ouzounova, K. Hinz, I. Wlodarska, B. Maes, A. Steyls, A. Driessen, G. Verhoef, P. Gaulard, et al.
Detection of t(11;18)(q21;q21) by interphase fluorescence in situ hybridization using API2 and MLT specific probes
Blood, September 15, 2000; 96(6): 2215 - 2218.
[Abstract] [Full Text] [PDF]

Home page
 ASH Education BookHome page
E. Macintyre, D. Willerford, and S. W. Morris
Non-Hodgkin's Lymphoma: Molecular Features of B Cell Lymphoma
Hematology, January 1, 2000; 2000(1): 180 - 204.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. J. Clem, T.-T. Sheu, B. W. M. Richter, W.-W. He, N. A. Thornberry, C. S. Duckett, and J. M. Hardwick
c-IAP1 Is Cleaved by Caspases to Produce a Proapoptotic C-terminal Fragment
J. Biol. Chem., March 2, 2001; 276(10): 7602 - 7608.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Society for Investigative Pathology.