help button home button Am J Pathol ASIP 2008 Summer Academy, Molecular Methcanisms of Human Disease: Injury, Inflammation, and Tissue Repair
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Klotz, L.
Right arrow Articles by Dombrowski, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Klotz, L.
Right arrow Articles by Dombrowski, F.
(American Journal of Pathology. 2000;156:1613-1626.)
© 2000 American Society for Investigative Pathology

Regular Articles

Hepatocellular Alterations after Intraportal Transplantation of Ovarian Tissue in Ovariectomized Rats

Luisa Klotz*, Hans Jörg Hacker{dagger}, Dietrich Klingmüller{ddagger}, Peter Bannasch{dagger}, Ulrich Pfeifer* and Frank Dombrowski*

From the Pathologisches Institut,*
Universität Bonn, Bonn; the Abteilung für Cytopathologie,{dagger}
Deutsches Krebsforschungszentrum, Heidelberg; and the Institut für Klinische Biochemie,{ddagger}
Universität Bonn, Bonn, Germany

The mechanisms of hepatocarcinogenesis by certain synthetic estrogens seem to involve both nongenotoxic and indirect genotoxic effects. However, the natural estrogen estradiol did not exert any carcinogenic effects in established experimental protocols. To elucidate specific long-term effects of natural estrogens on hepatocytes, small pieces of ovarian tissue were transplanted via the portal vein into the livers of ovariectomized female rats. One week, 3 weeks, and 3 months after transplantation the transplants were found to proliferate and to secrete estradiol. Three weeks after transplantation the hepatocytes of the liver acini downstream of the stimulated transplants already showed a remarkable loss of glycogen, distinct cytoplasmic amphophilia, enlargement of their nuclei, a strong increase in the number and size of peroxisomes, an increase in proliferative activity and apoptotic elimination, and changes in the activity of certain key enzymes of energy metabolism. All hepatocellular alterations could be inhibited by the estrogen receptor antagonist toremifene and are, therefore, attributed to specific effects of estradiol produced by the transplants. The observed alterations resemble in some respects amphophilic preneoplastic liver foci, which particularly occur after long-term administration of nongenotoxic hepatocarcinogens, including the adrenal steroid hormone dehydroepiandrosterone. In a preliminary experiment three of six animals exhibited a hepatocellular carcinoma, and another animal developed a hepatocellular adenoma 18 months after intrahepatic ovarian tissue transplantation.




This article has been cited by other articles:


Home page
 Toxicol PatholHome page
G. M. Ledda-Columbano, A. Perra, D. Concas, C. Cossu, F. Molotzu, C. Sartori, H. Shinozuka, and A. Columbano
Different Effects of the Liver Mitogens Triiodo-Thyronine and Ciprofibrate on the Development of Rat Hepatocellular Carcinoma
Toxicol Pathol, January 1, 2003; 31(1): 113 - 120.
[Abstract] [PDF]

Home page
 Arch SurgHome page
J. F. Kuebler, D. Jarrar, B. Toth, K. I. Bland, L. Rue III, P. Wang, and I. H. Chaudry
Estradiol Administration Improves Splanchnic Perfusion Following Trauma-Hemorrhage and Sepsis
Arch Surg, January 1, 2002; 137(1): 74 - 79.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Society for Investigative Pathology.