| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
From the Department of Clinical Molecular
Biology,*
Faculty of Medicine, Kyoto University, Kyoto and
the Department of Urology,
Faculty of
Medicine, Kansai Medical University, Osaka, Japan
Physiological scrotal hypothermia is necessary for normal spermatogenesis and fertility in mammals. Human RNA binding motif protein 3 (RBM3) is structurally highly similar to the cold-inducible RNA-binding protein (Cirp), and both mRNAs are induced in human cells at the scrotal temperature (32°C). We report here the cloning of mouse Rbm3 cDNA, which encoded an 18-kd protein with 94% identity in amino acid sequence to that of human RBM3. In the testis of adult mice, Rbm3 mRNA and protein were detected in Sertoli cells, but not germ cells, of seminiferous tubules at all stages. The expression was not observed in Sertoli cells of fetuses, but was observed in newborn and older mice. In the TAMA26 mouse Sertoli cell line, the Rbm3 expression level was increased or decreased within 12 hours after temperature shift from 37°C to 32°C or 39°C, respectively. In contrast to Cirp, the cold-induced growth suppression of TAMA26 cells was not affected by suppression of the Rbm3 expression. When mouse testis was exposed to heat stress by experimental cryptorchidism, the level of Rbm3 was decreased in Sertoli cells. Rbm3 may play important roles distinct from those played by Cirp in spermatogenesis and cryptorchidism by regulating the gene expression in Sertoli cells.
This article has been cited by other articles:
 |
|
 |
|
  J. Dresios, A. Aschrafi, G. C. Owens, P. W. Vanderklish, G. M. Edelman, and V. P. Mauro Cold stress-induced protein Rbm3 binds 60S ribosomal subunits, alters microRNA levels, and enhances global protein synthesis PNAS, February 8, 2005; 102(6): 1865 - 1870. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Y. Gracey, E. J. Fraser, W. Li, Y. Fang, R. R. Taylor, J. Rogers, A. Brass, and A. R. Cossins Coping with cold: An integrative, multitissue analysis of the transcriptome of a poikilothermic vertebrate PNAS, November 30, 2004; 101(48): 16970 - 16975. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Wellmann, C. Buhrer, E. Moderegger, A. Zelmer, R. Kirschner, P. Koehne, J. Fujita, and K. Seeger Oxygen-regulated expression of the RNA-binding proteins RBM3 and CIRP by a HIF-1-independent mechanism J. Cell Sci., May 1, 2004; 117(9): 1785 - 1794. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.J.I. Ellis, R.A. Furlong, A. Wilson, S. Morris, D. Carter, G. Oliver, C. Print, P.S. Burgoyne, K.L. Loveland, and N.A. Affara Modulation of the mouse testis transcriptome during postnatal development and in selected models of male infertility Mol. Hum. Reprod., April 1, 2004; 10(4): 271 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. J. Cok, S. J. Acton, A. E. Sexton, and A. R. Morrison Identification of RNA-binding Proteins in RAW 264.7 Cells That Recognize a Lipopolysaccharide-responsive Element in the 3-Untranslated Region of the Murine Cyclooxygenase-2 mRNA J. Biol. Chem., February 27, 2004; 279(9): 8196 - 8205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Shetty and C. C. Y. Weng Cryptorchidism Rescues Spermatogonial Differentiation in Juvenile Spermatogonial Depletion (Jsd) Mice Endocrinology, January 1, 2004; 145(1): 126 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kita, J. Carmichael, J. Swartz, S. Muro, A. Wyttenbach, K. Matsubara, D. C. Rubinsztein, and K. Kato Modulation of polyglutamine-induced cell death by genes identified by expression profiling Hum. Mol. Genet., September 15, 2002; 11(19): 2279 - 2287. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Sonna, J. Fujita, S. L. Gaffin, and C. M. Lilly Molecular Biology of Thermoregulation: Invited Review: Effects of heat and cold stress on mammalian gene expression J Appl Physiol, April 1, 2002; 92(4): 1725 - 1742. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.T.K. Saunders, S.M. Maguire, S. Macpherson, M.C. Fenelon, S. Sakakibara, and H. Okano RNA Binding Protein Musashi1 Is Expressed in Sertoli Cells in the Rat Testis from Fetal Life to Adulthood Biol Reprod, February 1, 2002; 66(2): 500 - 507. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Chappell, G. C. Owens, and V. P. Mauro A 5' Leader of Rbm3, a Cold Stress-induced mRNA, Mediates Internal Initiation of Translation with Increased Efficiency under Conditions of Mild Hypothermia J. Biol. Chem., September 28, 2001; 276(40): 36917 - 36922. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
