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From the Departments of Cardiovascular Research,*
Protein Chemistry,
Molecular
Biology,§
and Pathology,¶
Genentech, South San Francisco, California; and Curagen
Corporation,
New Haven, Connecticut
In the present study we have used a novel, comprehensive mRNA profiling technique (GeneCalling) for determining differential gene expression profiles of human endothelial cells undergoing differentiation into tubelike structures. One hundred fifteen cDNA fragments were identified and shown to represent 90 distinct genes. Although some of the genes identified have previously been implicated in angiogenesis, potential roles for many new genes, including OX-40, white protein homolog, KIAA0188, a homolog of angiopoietin-2, ADAMTS-4 (aggrecanase-1), and stanniocalcin were revealed. Support for the biological significance was confirmed by the abrogation of the changes in the expression of angiogenesis inhibitors and in situ hybridization studies. This study has significantly extends the molecular fingerprint of the changes in gene expression that occur during endothelial differentiation and provides new insights into the potential role of a number of new molecules in angiogenesis.
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H. Li, S. Brodsky, S. Kumari, V. Valiunas, P. Brink, J.-I. Kaide, A. Nasjletti, and M. S. Goligorsky Paradoxical overexpression and translocation of connexin43 in homocysteine-treated endothelial cells Am J Physiol Heart Circ Physiol, June 1, 2002; 282(6): H2124 - H2133. [Abstract] [Full Text] [PDF]
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H. Li, A. Lewis, S. Brodsky, R. Rieger, C. Iden, and M. S. Goligorsky Homocysteine Induces 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase in Vascular Endothelial Cells: A Mechanism for Development of Atherosclerosis? Circulation, March 5, 2002; 105(9): 1037 - 1043. [Abstract] [Full Text] [PDF]
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