| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
From the Departments of Physiological Science*
and Pathology and Laboratory Medicine,
University of California, Los Angeles, California
Modified muscle use can result in muscle inflammation that is triggered by unidentified events. In the present investigation, we tested whether the activation of the complement system is a component of muscle inflammation that results from changes in muscle loading. Modified rat hindlimb muscle loading was achieved by removing weight-bearing from the hindlimbs for 10 days followed by reloading through normal ambulation. Experimental animals were injected with the recombinant, soluble complement receptor sCR1 to inhibit complement activation. Assays for complement C4 or factor B in sera showed that sCR1 produced large reductions in the capacity for activation of the complement system through both the classical and alternative pathways. Analysis of complement C4 concentration in serum in untreated animals showed that the classical pathway was activated during the first 2 hours of reloading. Analysis of factor B concentration in untreated animals showed activation of the alternative pathway at 6 hours of reloading. Administration of sCR1 significantly attenuated the invasion of neutrophils (-49%) and ED1+ macrophages (-52%) that occurred in nontreated animals after 6 hours of reloading. The presence of sCR1 also reduced significantly the degree of edema by 22% as compared to untreated animals. Together, these data show that increased muscle loading activated the complement system which then briefly contributes to the early recruitment of inflammatory cells during modified muscle loading.
This article has been cited by other articles:
 |
|
 |
|
  N. Dumont, K. Lepage, C. H. Cote, and J. Frenette Mast cells can modulate leukocyte accumulation and skeletal muscle function following hindlimb unloading J Appl Physiol, July 1, 2007; 103(1): 97 - 104. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Tidball and M. Wehling-Henricks Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo J. Physiol., January 1, 2007; 578(1): 327 - 336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. McClung, J. M. Davis, and J. A. Carson Muscle: Ovarian hormone status and skeletal muscle inflammation during recovery from disuse in rats Exp Physiol, January 1, 2007; 92(1): 219 - 232. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. McClung, J. M. Davis, M. A. Wilson, E. C. Goldsmith, and J. A. Carson Estrogen status and skeletal muscle recovery from disuse atrophy J Appl Physiol, June 1, 2006; 100(6): 2012 - 2023. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Bondesen, S. T. Mills, and G. K. Pavlath The COX-2 pathway regulates growth of atrophied muscle via multiple mechanisms Am J Physiol Cell Physiol, June 1, 2006; 290(6): C1651 - C1659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Van Gammeren, D. J. Falk, K. C. DeRuisseau, J. E. Sellman, M. Decramer, and S. K. Powers Reloading the Diaphragm Following Mechanical Ventilation Does Not Promote Injury Chest, June 1, 2005; 127(6): 2204 - 2210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. McClung, K. A. Mehl, R. W. Thompson, L. L. Lowe, and J. A. Carson Nandrolone decanoate modulates cell cycle regulation in functionally overloaded rat soleus muscle Am J Physiol Regulatory Integrative Comp Physiol, June 1, 2005; 288(6): R1543 - R1552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. X. Nguyen, A. J. Lusis, and J. G. Tidball Null mutation of myeloperoxidase in mice prevents mechanical activation of neutrophil lysis of muscle cell membranes in vitro and in vivo J. Physiol., June 1, 2005; 565(2): 403 - 413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. G. Tidball Inflammatory processes in muscle injury and repair Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2005; 288(2): R345 - R353. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Frenette, N. Chbinou, C. Godbout, D. Marsolais, and P. S. Frenette Macrophages, not neutrophils, infiltrate skeletal muscle in mice deficient in P/E selectins after mechanical reloading Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2003; 285(4): R727 - R732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. M. Lapointe, J. Frenette, and C. H. Cote Lengthening contraction-induced inflammation is linked to secondary damage but devoid of neutrophil invasion J Appl Physiol, May 1, 2002; 92(5): 1995 - 2004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. B. HUNTER, E. J. STEVENSON, A. KONCAREVIC, H. MITCHELL-FELTON, D. A. ESSIG, and S. C. KANDARIAN Activation of an alternative NF-{kappa}B pathway in skeletal muscle during disuse atrophy FASEB J, April 1, 2002; 16(6): 529 - 538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Frenette, M. St-Pierre, C. H. Cote, E. Mylona, and F. X. Pizza Muscle impairment occurs rapidly and precedes inflammatory cell accumulation after mechanical loading Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2002; 282(2): R351 - R357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. X. Pizza, T. J. McLoughlin, S. J. McGregor, E. P. Calomeni, and W. T. Gunning Neutrophils injure cultured skeletal myotubes Am J Physiol Cell Physiol, July 1, 2001; 281(1): C335 - C341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. X. Pizza, T. J. Koh, S. J. McGregor, and S. V. Brooks Muscle inflammatory cells after passive stretches, isometric contractions, and lengthening contractions J Appl Physiol, May 1, 2002; 92(5): 1873 - 1878. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
