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(American Journal of Pathology. 2000;157:171-175.)
© 2000 American Society for Investigative Pathology

Regular Articles

CD8+ T Cells in Hodgkin’s Disease Tumor Tissue Are a Polyclonal Population with Limited Clonal Expansion but Little Evidence of Selection by Antigen

Klaus Willenbrock*, Axel Roers{dagger}, Birgit Blöhbaum*, Klaus Rajewsky{dagger} and Martin-Leo Hansmann*

From the Institute for Pathology,*
University of Frankfurt, Frankfurt am Main; and the Institute for Genetics,{dagger}
University of Cologne, Cologne, Germany

A minor component (about 25%) of lymphocytes in Hodgkin’s disease (HD) are CD8+ T cells. It is unclear whether the presence of these cells reflects an antitumor cytotoxic response. The goal of the present study was to investigate clonal composition and the T cell receptor (TCR) ß repertoire of the CD8+ T cell population in HD. Single CD8+ cells were micromanipulated from frozen tissue sections of lymph nodes affected by primary HD and subjected to single target amplification of TCRß gene rearrangements. Sequence analysis of the V region genes revealed the presence of expanded CD8+ T cell clones in all three cases analyzed. Most of these clonal expansions accounted for less than 10% of the CD8+ T cell population. In one case, 30% of the CD8+ T cells belonged to one or two clones. Comparison of V region sequences, however, did not provide evidence that the micromanipulated CD8+ cells were sampled from a population that was selected for particular antigen specificities. No obvious biases in TCR Vß and Jß gene segment usage or CDR3 length distribution were found. Similarities of CDR3 amino acid sequences as found in selected CDR3 structures were rare. These results suggest that, like CD4+ T cells, CD8+ T cells may also be recruited into the tumor tissue in an antigen-nonspecific manner.




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