help button home button Am J Pathol Epitomics, Inc.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mahooti, S.
Right arrow Articles by Madri, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mahooti, S.
Right arrow Articles by Madri, J. A.
(American Journal of Pathology. 2000;157:75-81.)
© 2000 American Society for Investigative Pathology

Short Communications

PECAM-1 (CD31) Expression Modulates Bleeding Time in Vivo

Sepi Mahooti*, Donnasue Graesser*, Sonali Patil{dagger}, Peter Newman{dagger}, Gordon Duncan{ddagger}, Tak Mak{ddagger} and Joseph A. Madri*

From the Department of Pathology,*
Yale University School of Medicine, New Haven, Connecticut; the Blood Research Institute,{dagger}
Blood Center of Southeastern Wisconsin, Milwaukee, Wisconsin; and the AMGEN Institute,{ddagger}
University of Toronto, Toronto, Ontario, Canada

PECAM-1 is a 130-kd member of the Ig superfamily present on endothelial cells, platelets, polymorphonuclear leukocytes, monocytes, and lymphocytes. Its expression begins early in development and persists through adulthood. PECAM-1 functions as an adhesion and signaling molecule between adjacent endothelial cells and between endothelial cells and circulating blood elements. Antibodies directed against PECAM-1 have been shown to affect angiogenesis, endothelial cell migration, and polymorphonuclear leukocyte transmigration. Furthermore, its dimerization is associated with the modulation of integrin affinity. Antibody inhibition studies suggest that PECAM-1 plays a role in modulating thrombosis; however, recent in vitro aggregation studies performed on platelets harvested from PECAM-1-deficient mice revealed no abnormalities. In this report we demonstrate prolonged in vivo bleeding times in PECAM-1-deficient mice. This abnormality was not corrected when wild-type hematopoietic precursors were engrafted into marrow-ablated PECAM-1-deficient mice. Furthermore, normal bleeding times were observed when marrow-ablated wild-type mice were engrafted with hematopoietic precursors harvested from PECAM-1-deficient mice. These studies are consistent with a role for PECAM-1 in modulating thrombosis in the vasculature, which is potentially mediated by endothelial cell PECAM-1 expression.




This article has been cited by other articles:


Home page
 Am. J. Pathol.Home page
G. Cao, M. L. Fehrenbach, J. T. Williams, J. M. Finklestein, J.-X. Zhu, and H. M. DeLisser
Angiogenesis in Platelet Endothelial Cell Adhesion Molecule-1-Null Mice
Am. J. Pathol., August 1, 2009; 175(2): 903 - 915.
[Abstract] [Full Text] [PDF]

Home page
 Arterioscler. Thromb. Vasc. Bio.Home page
Z. Chen and E. Tzima
PECAM-1 Is Necessary for Flow-Induced Vascular Remodeling
Arterioscler Thromb Vasc Biol, July 1, 2009; 29(7): 1067 - 1073.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
Y. Wu, K. Tworkoski, M. Michaud, and J. A. Madri
Bone Marrow Monocyte PECAM-1 Deficiency Elicits Increased Osteoclastogenesis Resulting in Trabecular Bone Loss
J. Immunol., March 1, 2009; 182(5): 2672 - 2679.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
C. Garnacho, V. Shuvaev, A. Thomas, L. McKenna, J. Sun, M. Koval, S. Albelda, V. Muzykantov, and S. Muro
RhoA activation and actin reorganization involved in endothelial CAM-mediated endocytosis of anti-PECAM carriers: critical role for tyrosine 686 in the cytoplasmic tail of PECAM-1
Blood, March 15, 2008; 111(6): 3024 - 3033.
[Abstract] [Full Text] [PDF]

Home page
 JEMHome page
P. Baluk, J. Fuxe, H. Hashizume, T. Romano, E. Lashnits, S. Butz, D. Vestweber, M. Corada, C. Molendini, E. Dejana, et al.
Functionally specialized junctions between endothelial cells of lymphatic vessels
J. Exp. Med., October 1, 2007; 204(10): 2349 - 2362.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Wu, T. Welte, M. Michaud, and J. A. Madri
PECAM-1: a multifaceted regulator of megakaryocytopoiesis
Blood, August 1, 2007; 110(3): 851 - 859.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
G. Cao, R. C. Savani, M. Fehrenbach, C. Lyons, L. Zhang, G. Coukos, and H. M. DeLisser
Involvement of Endothelial CD44 during in Vivo Angiogenesis
Am. J. Pathol., July 1, 2006; 169(1): 325 - 336.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Falati, S. Patil, P. L. Gross, M. Stapleton, G. Merrill-Skoloff, N. E. Barrett, K. L. Pixton, H. Weiler, B. Cooley, D. K. Newman, et al.
Platelet PECAM-1 inhibits thrombus formation in vivo
Blood, January 15, 2006; 107(2): 535 - 541.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
J. L. Wee and D. E. Jackson
The Ig-ITIM superfamily member PECAM-1 regulates the "outside-in" signaling properties of integrin {alpha}IIb{beta}3 in platelets
Blood, December 1, 2005; 106(12): 3816 - 3823.
[Abstract] [Full Text] [PDF]

Home page
 Infect. Immun.Home page
P. L. W. Yun, A. A. Decarlo, C. C. Chapple, and N. Hunter
Functional Implication of the Hydrolysis of Platelet Endothelial Cell Adhesion Molecule 1 (CD31) by Gingipains of Porphyromonas gingivalis for the Pathology of Periodontal Disease
Infect. Immun., March 1, 2005; 73(3): 1386 - 1398.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. Maas, M. Stapleton, C. Bergom, D. L. Mattson, D. K. Newman, and P. J. Newman
Endothelial cell PECAM-1 confers protection against endotoxic shock
Am J Physiol Heart Circ Physiol, January 1, 2005; 288(1): H159 - H164.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. R. Schenkel, T. W. Chew, and W. A. Muller
Platelet Endothelial Cell Adhesion Molecule Deficiency or Blockade Significantly Reduces Leukocyte Emigration in a Majority of Mouse Strains
J. Immunol., November 15, 2004; 173(10): 6403 - 6408.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
J. ESPARZA, M. KRUSE, J. LEE, M. MICHAUD, and J. A. MADRI
MMP-2 null mice exhibit an early onset and severe experimental autoimmune encephalomyelitis due to an increase in MMP-9 expression and activity
FASEB J, November 1, 2004; 18(14): 1682 - 1691.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
C. D. O'Brien, G. Cao, A. Makrigiannakis, and H. M. DeLisser
Role of immunoreceptor tyrosine-based inhibitory motifs of PECAM-1 in PECAM-1-dependent cell migration
Am J Physiol Cell Physiol, October 1, 2004; 287(4): C1103 - C1113.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
S. M. Albelda, K. C. Lau, P. Chien, Z.-Y. Huang, E. Arguiris, A. Bohen, J. Sun, J. A. Billet, M. Christofidou-Solomidou, Z. K. Indik, et al.
Role for Platelet-Endothelial Cell Adhesion Molecule-1 in Macrophage Fc{gamma} Receptor Function
Am. J. Respir. Cell Mol. Biol., August 1, 2004; 31(2): 246 - 255.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
D. GRATZINGER, S. CANOSA, B. ENGELHARDT, and J. A. MADRI
Platelet endothelial cell adhesion molecule-1 modulates endothelial cell motility through the small G-protein Rho
FASEB J, August 1, 2003; 17(11): 1458 - 1469.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
T. R. Kyriakides, P. Rojnuckarin, M. A. Reidy, K. D. Hankenson, T. Papayannopoulou, K. Kaushansky, and P. Bornstein
Megakaryocytes require thrombospondin-2 for normal platelet formation and function
Blood, May 15, 2003; 101(10): 3915 - 3923.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. Solowiej, P. Biswas, D. Graesser, and J. A. Madri
Lack of Platelet Endothelial Cell Adhesion Molecule-1 Attenuates Foreign Body Inflammation because of Decreased Angiogenesis
Am. J. Pathol., March 1, 2003; 162(3): 953 - 962.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
J. M. Enciso, D. Gratzinger, T. D. Camenisch, S. Canosa, E. Pinter, and J. A. Madri
Elevated glucose inhibits VEGF-A-mediated endocardial cushion formation: modulation by PECAM-1 and MMP-2
J. Cell Biol., February 18, 2003; 160(4): 605 - 615.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
S. Tasaka, L. Qin, A. Saijo, S. M. Albelda, H. M. DeLisser, and C. M. Doerschuk
Platelet Endothelial Cell Adhesion Molecule-1 in Neutrophil Emigration during Acute Bacterial Pneumonia in Mice and Rats
Am. J. Respir. Crit. Care Med., January 15, 2003; 167(2): 164 - 170.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
M. Osawa, M. Masuda, K.-i. Kusano, and K. Fujiwara
Evidence for a role of platelet endothelial cell adhesion molecule-1 in endothelial cell mechanosignal transduction: is it a mechanoresponsive molecule?
J. Cell Biol., August 19, 2002; 158(4): 773 - 785.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F. Benavides, M. F. Starost, M. Flores, I. B. Gimenez-Conti, J.-L. Guenet, and C. J. Conti
Impaired Hair Follicle Morphogenesis and Cycling with Abnormal Epidermal Differentiation in nackt Mice, a Cathepsin L-Deficient Mutation
Am. J. Pathol., August 1, 2002; 161(2): 693 - 703.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
K. L. Jones, S. C. Hughan, S. M. Dopheide, R. W. Farndale, S. P. Jackson, and D. E. Jackson
Platelet endothelial cell adhesion molecule-1 is a negative regulator of platelet-collagen interactions
Blood, September 1, 2001; 98(5): 1456 - 1463.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
S. Patil, D. K. Newman, and P. J. Newman
Platelet endothelial cell adhesion molecule-1 serves as an inhibitory receptor that modulates platelet responses to collagen
Blood, March 15, 2001; 97(6): 1727 - 1732.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. Cao, C. D. O'Brien, Z. Zhou, S. M. Sanders, J. N. Greenbaum, A. Makrigiannakis, and H. M. DeLisser
Involvement of human PECAM-1 in angiogenesis and in vitro endothelial cell migration
Am J Physiol Cell Physiol, May 1, 2002; 282(5): C1181 - C1190.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2000 by the American Society for Investigative Pathology.