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(American Journal of Pathology. 2000;157:463-471.)
© 2000 American Society for Investigative Pathology

Regular Articles

In Vivo Detection of Vascular Adhesion Protein-1 in Experimental Inflammation

Kimmo Jaakkola^*, Tuomo Nikula^¶, Riikka Holopainen^||, Tommi Vähäsilta^**, Marja-Terttu Matikainen, Marja-Leena Laukkanen, Risto Huupponen^§, Lauri Halkola^||, Lauri Nieminen^§§, Jukka Hiltunen^¶, Sakari Parviainen^¶¶, Michael R. Clark, Juhani Knuuti, Timo Savunen^**, Pekka Kääpä^||, Liisa Maria Voipio-Pulkki and Sirpa Jalkanen^*

From the National Public Health Institute and MediCity Research Laboratory,^*
the Departments of Medicine,
Nuclear Medicine,
and Pharmacology and Clinical Pharmacology,^§
the University of Turku, Turku, Finland; MAP Medical Technologies Inc.,^¶
Tikkakoski, Finland; the Cardiorespiratory Research Unit,^||
the Department of Surgery,^**
and the Centre for Biotechnology,
Åbo Academy University and University of Turku, Turku, Finland; the Department of Pathology,
Immunology Division, Cambridge University, Cambridge, United Kingdom; the Orion Corporation,^§§
Orion Pharma, Turku, Finland; and the Department of Nuclear Medicine,^¶¶
Turku University Central Hospital, Turku, Finland

Vascular adhesion protein-1 (VAP-1) is an inflammation-inducible endothelial glycoprotein which mediates leukocyte-endothelial cell interactions. To study the pathogenetic significance of VAP-1 in inflammatory disorders, an in vivo immunodetection method was used to detect the regulation of luminally expressed VAP-1 in experimental skin and joint inflammation in the pig and dog. Moreover, VAP-1 was studied as a potential target to localize inflammation by radioimmunoscintigraphy. Up-regulation of VAP-1 in experimental dermatitis and arthritis could be visualized by specifically targeted immunoscintigraphy. Moreover, the translocation of VAP-1 to the functional position on the endothelial surface was only seen in inflamed tissues. These results suggest that VAP-1 is both an optimal candidate for anti-adhesive therapy and a potential target molecule for imaging inflammation.

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