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(American Journal of Pathology. 2000;157:723-728.)
© 2000 American Society for Investigative Pathology

Technical Advance

The Development of a Cell Array and Its Combination with Laser-Scanning Cytometry Allows a High-Throughput Analysis of Nuclear DNA Content

Kenta Oode*, Tomoko Furuya*, Kei Harada*, Shigeto Kawauchi*, Koutaro Yamamoto{dagger}, Takashi Hirano{ddagger} and Kohsuke Sasaki*

From the Departments of Pathology*
and Surgery,{dagger}
Yamaguchi University School of Medicine, Ube; and the National Institute of Bioscience and Human Technology,{ddagger}
Tsukuba, Ibaraki, Japan

We developed a cell array device for analyzing cellular characteristics such as DNA ploidy, numerical chromosomal aberrations, and antigen expression in multiple specimens in a single experiment. Fifty (10 x 5) spots, 2 mm in diameter, were arrayed in an area of 30 x 16 mm on a glass slide, and ~1,000 cells were placed on each spot. To demonstrate the usefulness of the cell array, we measured nuclear DNA content using laser-scanning cytometry for DNA ploidy analysis in nine human tumor cell lines and normal lymphocytes. Combining the cell array with laser-scanning cytometry allows not only measurement of nuclear DNA content for 50 samples but also easy comparison of DNA ploidy among the samples in a single experiment. In addition, we used the cell array for fluorescence in situ hybridization using a DNA probe specific for the pericentromeric region of chromosome 11.




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