This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Zhang, W. Articles by Yan, S.-F. Search for Related Content PubMed PubMed Citation Articles by Zhang, W. Articles by Yan, S.-F.

(American Journal of Pathology. 2000;157:1311-1320.)
© 2000 American Society for Investigative Pathology

Regular Articles

Expression of Egr-1 in Late Stage Emphysema

Weisu Zhang^*, Shi Du Yan, Aiping Zhu^*, Yu Shan Zou^*, Matthew Williams^*, Gabriel C. Godman, Byron M. Thomashow, Mark E. Ginsburg^*, David M. Stern^*§ and Shi-Fang Yan^*

From the Departments of Surgery,^*
Pathology,
Medicine,
and Physiology and Cellular Biophysics,^§
College of Physicians and Surgeons of Columbia University, New York, New York

The transcription factor early growth response (Egr)-1 is an immediate-early gene product rapidly and transiently expressed after acute tissue injury. In contrast, in this report we demonstrate that lung tissue from patients undergoing lung reduction surgery for advanced emphysema, without clinical or anatomical evidence of acute infection, displays a selective and apparently sustained increase in Egr-1 transcripts and antigen, compared with a broad survey of other genes, including the transcription factor Sp1, whose levels were not significantly altered. Enhanced Egr-1 expression was especially evident in smooth muscle cells of bronchial and vascular walls, in alveolar macrophages, and some vascular endothelium. Gel shift analysis with ³²P-labeled Egr probe showed a band with nuclear extracts from emphysematous lung which was supershifted with antibody to Egr-1. Egr-1 has the capacity to regulate genes relevant to the pathophysiology of emphysema, namely those related to extracellular matrix formation and remodeling, thrombogenesis, and those encoding cytokines/chemokines and growth factors. Thus, we propose that further analysis of Egr-1, which appears to be up-regulated in a sustained fashion in patients with late stage emphysema, may provide insights into the pathogenesis of this destructive pulmonary disease, as well as a new facet in the biology of Egr-1.

This article has been cited by other articles:

				H. Yasuoka, E. Hsu, X. D. Ruiz, R. A. Steinman, A. M.K. Choi, and C. A. Feghali-Bostwick The Fibrotic Phenotype Induced by IGFBP-5 Is Regulated by MAPK Activation and Egr-1-Dependent and -Independent Mechanisms Am. J. Pathol., August 1, 2009; 175(2): 605 - 615. [Abstract] [Full Text] [PDF]

				T. Rangasamy, V. Misra, L. Zhen, C. G. Tankersley, R. M. Tuder, and S. Biswal Cigarette smoke-induced emphysema in A/J mice is associated with pulmonary oxidative stress, apoptosis of lung cells, and global alterations in gene expression Am J Physiol Lung Cell Mol Physiol, June 1, 2009; 296(6): L888 - L900. [Abstract] [Full Text] [PDF]

				S. Bhattacharya, S. Srisuma, D. L. DeMeo, S. D. Shapiro, R. Bueno, E. K. Silverman, J. J. Reilly, and T. J. Mariani Molecular Biomarkers for Quantitative and Discrete COPD Phenotypes Am. J. Respir. Cell Mol. Biol., March 1, 2009; 40(3): 359 - 367. [Abstract] [Full Text] [PDF]

				S. Bhattacharyya, S.-J. Chen, M. Wu, M. Warner-Blankenship, H. Ning, G. Lakos, Y. Mori, E. Chang, C. Nihijima, K. Takehara, et al. Smad-Independent Transforming Growth Factor-{beta} Regulation of Early Growth Response-1 and Sustained Expression in Fibrosis: Implications for Scleroderma Am. J. Pathol., October 1, 2008; 173(4): 1085 - 1099. [Abstract] [Full Text] [PDF]

				W. Ning, Y. Dong, J. Sun, C. Li, M. A. Matthay, C. A. Feghali-Bostwick, and A. M. K. Choi Cigarette Smoke Stimulates Matrix Metalloproteinase-2 Activity via EGR-1 in Human Lung Fibroblasts Am. J. Respir. Cell Mol. Biol., April 1, 2007; 36(4): 480 - 490. [Abstract] [Full Text] [PDF]

				S.-F. Yan, E. Harja, M. Andrassy, T. Fujita, and A. M. Schmidt Protein Kinase C {beta}/Early Growth Response-1 Pathway: A Key Player in Ischemia, Atherosclerosis, and Restenosis J. Am. Coll. Cardiol., October 27, 2006; 48(9_Suppl_A): A47 - A55. [Abstract] [Full Text] [PDF]

				J. L. Ingram, A. Antao-Menezes, J. B. Mangum, O. Lyght, P. J. Lee, J. A. Elias, and J. C. Bonner Opposing Actions of Stat1 and Stat6 on IL-13-Induced Up-Regulation of Early Growth Response-1 and Platelet-Derived Growth Factor Ligands in Pulmonary Fibroblasts J. Immunol., September 15, 2006; 177(6): 4141 - 4148. [Abstract] [Full Text] [PDF]

				P. R. Reynolds, M. G. Cosio, and J. R. Hoidal Cigarette Smoke-Induced Egr-1 Upregulates Proinflammatory Cytokines in Pulmonary Epithelial Cells Am. J. Respir. Cell Mol. Biol., September 1, 2006; 35(3): 314 - 319. [Abstract] [Full Text] [PDF]

				J.-H. Kim, K. Yamaguchi, S.-H. Lee, P. K. Tithof, G. S. Sayler, J.-H. Yoon, and S. J. Baek Evaluation of Polycyclic Aromatic Hydrocarbons in the Activation of Early Growth Response-1 and Peroxisome Proliferator Activated Receptors Toxicol. Sci., May 1, 2005; 85(1): 585 - 593. [Abstract] [Full Text] [PDF]

				J. M. Martinez, S. J. Baek, D. M. Mays, P. K. Tithof, T. E. Eling, and N. J. Walker EGR1 Is a Novel Target for AhR Agonists in Human Lung Epithelial Cells Toxicol. Sci., December 1, 2004; 82(2): 429 - 435. [Abstract] [Full Text] [PDF]

				A. Spira, J. Beane, V. Pinto-Plata, A. Kadar, G. Liu, V. Shah, B. Celli, and J. S. Brody Gene Expression Profiling of Human Lung Tissue from Smokers with Severe Emphysema Am. J. Respir. Cell Mol. Biol., December 1, 2004; 31(6): 601 - 610. [Abstract] [Full Text] [PDF]

				W. Ning, C.-J. Li, N. Kaminski, C. A. Feghali-Bostwick, S. M. Alber, Y. P. Di, S. L. Otterbein, R. Song, S. Hayashi, Z. Zhou, et al. Comprehensive gene expression profiles reveal pathways related to the pathogenesis of chronic obstructive pulmonary disease PNAS, October 12, 2004; 101(41): 14895 - 14900. [Abstract] [Full Text] [PDF]

				C. G. Lee, S. J. Cho, M. J. Kang, S. P. Chapoval, P. J. Lee, P. W. Noble, T. Yehualaeshet, B. Lu, R. A. Flavell, J. Milbrandt, et al. Early Growth Response Gene 1-mediated Apoptosis Is Essential for Transforming Growth Factor {beta}1-induced Pulmonary Fibrosis J. Exp. Med., August 2, 2004; 200(3): 377 - 389. [Abstract] [Full Text] [PDF]

				M. Shozu, K. Murakami, T. Segawa, T. Kasai, H. Ishikawa, K. Shinohara, M. Okada, and M. Inoue Decreased Expression of Early Growth Response-1 and Its Role in Uterine Leiomyoma Growth Cancer Res., July 1, 2004; 64(13): 4677 - 4684. [Abstract] [Full Text] [PDF]

				J. Hjoberg, L. Le, A. Imrich, V. Subramaniam, S. I. Mathew, J. Vallone, K. J. Haley, F. H. Y. Green, S. A. Shore, and E. S. Silverman Induction of early growth-response factor 1 by platelet-derived growth factor in human airway smooth muscle Am J Physiol Lung Cell Mol Physiol, April 1, 2004; 286(4): L817 - L825. [Abstract] [Full Text] [PDF]

				E. Harja, L. G. Bucciarelli, Y. Lu, D. M. Stern, Y. S. Zou, A. M. Schmidt, and S.-F. Yan Early Growth Response-1 Promotes Atherogenesis: Mice Deficient in Early Growth Response-1 and Apolipoprotein E Display Decreased Atherosclerosis and Vascular Inflammation Circ. Res., February 20, 2004; 94(3): 333 - 339. [Abstract] [Full Text] [PDF]