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(American Journal of Pathology. 2001;158:317-321.)
© 2001 American Society for Investigative Pathology

Regular Articles

Molecular Characterization of Pancreatic Serous Microcystic Adenomas

Evidence for a Tumor Suppressor Gene on Chromosome 10q

Patrick S. Moore^*, Giuseppe Zamboni^*, Antonietta Brighenti^*, Daniele Lissandrini^*, Davide Antonello^*, Paola Capelli^*, Gildas Rigaud^*, Massimo Falconi and Aldo Scarpa^*

From the Departments of Pathology^*
and Surgery,
Università di Verona, Verona, Italy

Pancreatic serous microcystic adenomas (SCAs) are rare, benign tumors with a striking female preference. Virtually no information is available about chromosomal or genetic anomalies in this disease. We performed extensive molecular characterization of 21 cases of formalin-fixed, paraffin-embedded sporadic SCAs consisting in genome-wide allelic loss analysis with 79 microsatellite markers covering all 22 autosomes, assessment of microsatellite instability, and mutational analysis of the VHL, K-ras, and p53 genes in nine cases for which frozen tissue was available. Although no case showed microsatellite instability of the type seen in mismatch repair-deficient tumors, a relatively low fractional allelic loss of 0.08 was found. Losses on chromosome 10q were the most frequent event in SCAs (50% of cases), followed by allelic losses on chromosome 3p (40% of cases). Moderately frequent losses (>25% of cases) were found on chromosomes 1q, 2q, and 7q. The VHL gene, located on chromosome 3p, had somatic inactivating mutations in two of nine cases (22%), whereas no mutations were found in either K-ras or p53, in agreement with the finding that all 21 cases stained negative for p53 by immunohistochemistry. Our study indicates that the involvement of chromosomal arms 10q and 3p is characteristic of SCAs and that the VHL gene is involved in a subset of sporadic cases.

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