This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tanière, P. Articles by Hainaut, P. Search for Related Content PubMed PubMed Citation Articles by Tanière, P. Articles by Hainaut, P.

(American Journal of Pathology. 2001;158:33-40.)
© 2001 American Society for Investigative Pathology

Short Communications

Molecular and Clinical Differences between Adenocarcinomas of the Esophagus and of the Gastric Cardia

Philippe Tanière^*, Ghislaine Martel-Planche^*, Daniela Maurici^*, Catherine Lombard-Bohas, Jean-Yves Scoazec, Ruggero Montesano^*, Françoise Berger and Pierre Hainaut^*

From the International Agency for Research on Cancer;^*
the Fédération des Spécialités Digestives
and the Laboratoire d’Anatomie Pathologique,
Hôpital Edouard Herriot, Lyon, France

Adenocarcinoma of the esophagus (ADCE) with Barrett’s mucosa and adenocarcinoma of the cardia (ADCC) are often reported as a single pathological entity. In this study we have used strict anatomical-pathological criteria to distinguish between these two lesions and we have investigated their differences in TP53 mutations, MDM2 gene amplification, and cytokeratin expression. DNA was extracted from the tumor areas of formalin-fixed, paraffin-embedded sections in 26 ADCC and 28 ADCE patients. TP53 mutations were detected by temporal temperature gradient electrophoresis and identified by sequencing. MDM2 amplification was assessed by differential polymerase chain reaction. The expression of cytokeratins 4, 7, and 13 was examined by immunohistochemistry. In ADCC, the male to female ratio was 1.8:1, compared to 27:1 in ADCE. Five ADCC patients had a history of other neoplasms, compared to only one ADCE patient. The two types of tumor differed in the prevalence of TP53 mutations (31% in ADCC and 50% in ADCE) and of MDM2 gene amplification (19% in ADCC and 4% in ADCE), and in the pattern of expression of cytokeratin 7 (positive in 100% of ADCE and in 41% of ADCC) and cytokeratin 13 (positive in 81% of ADCE and in 36.5% of ADCC). ADCE and ADCC differ in their clinical characteristics, in the prevalence of TP53 mutations and MDM2 amplifications, and in the patterns of cytokeratin expression. These results support the notion that ADCC and ADCE are distinct pathological entities.

This article has been cited by other articles:

				S.-H. Kim, H. Nakagawa, A. Navaraj, Y. Naomoto, A. J.P. Klein-Szanto, A. K. Rustgi, and W. S. El-Deiry Tumorigenic Conversion of Primary Human Esophageal Epithelial Cells Using Oncogene Combinations in the Absence of Exogenous Ras Cancer Res., November 1, 2006; 66(21): 10415 - 10424. [Abstract] [Full Text] [PDF]

				F. Kamangar, S. M. Dawsey, M. J. Blaser, G. I. Perez-Perez, P. Pietinen, C. J. Newschaffer, C. C. Abnet, D. Albanes, J. Virtamo, and P. R. Taylor Opposing Risks of Gastric Cardia and Noncardia Gastric Adenocarcinomas Associated With Helicobacter pylori Seropositivity. J Natl Cancer Inst, October 18, 2006; 98(20): 1445 - 1452. [Abstract] [Full Text] [PDF]

				R. F. Souza and S. J. Spechler Concepts in the Prevention of Adenocarcinoma of the Distal Esophagus and Proximal Stomach CA Cancer J Clin, November 1, 2005; 55(6): 334 - 351. [Abstract] [Full Text] [PDF]

				F. Le Calvez, A. Mukeria, J. D. Hunt, O. Kelm, R. J. Hung, P. Taniere, P. Brennan, P. Boffetta, D. G. Zaridze, and P. Hainaut TP53 and KRAS Mutation Load and Types in Lung Cancers in Relation to Tobacco Smoke: Distinct Patterns in Never, Former, and Current Smokers Cancer Res., June 15, 2005; 65(12): 5076 - 5083. [Abstract] [Full Text] [PDF]

				C J Buskens, A Sivula, B P van Rees, C Haglund, G J A Offerhaus, J J B van Lanschot, and A Ristimaki Comparison of cyclooxygenase 2 expression in adenocarcinomas of the gastric cardia and distal oesophagus Gut, December 1, 2003; 52(12): 1678 - 1683. [Abstract] [Full Text] [PDF]

				C. T. Miller, S. Aggarwal, T. K. Lin, S. L. Dagenais, J. I. Contreras, M. B. Orringer, T. W. Glover, D. G. Beer, and L. Lin Amplification and Overexpression of the Dual-Specificity Tyrosine-(Y)-Phosphorylation Regulated Kinase 2 (DYRK2) Gene in Esophageal and Lung Adenocarcinomas Cancer Res., July 15, 2003; 63(14): 4136 - 4143. [Abstract] [Full Text] [PDF]

				A. G. Casson, S. C. Evans, A. Gillis, G. A. Porter, P. Veugelers, S. J. Darnton, D. L. Guernsey, and P. Hainaut Clinical implications of p53 tumor suppressor gene mutation and protein expression in esophageal adenocarcinomas: Results of a ten-year prospective study J. Thorac. Cardiovasc. Surg., May 1, 2003; 125(5): 1121 - 1131. [Abstract] [Full Text] [PDF]

				G. T. Y. Chung, Y. Yamada, R. Pannell, A. Forster, and T. H. Rabbitts The hepatitis C virus internal ribosome entry site facilitates efficient protein synthesis in blood vessel endothelium during tumour angiogenesis Nucleic Acids Res., April 15, 2003; 31(8): e46 - e46. [Abstract] [Full Text] [PDF]

				H. van Dekken, J. C. Alers, P. H. J. Riegman, C. Rosenberg, H. W. Tilanus, and K. Vissers Molecular Cytogenetic Evaluation of Gastric Cardia Adenocarcinoma and Precursor Lesions Am. J. Pathol., June 1, 2001; 158(6): 1961 - 1967. [Abstract] [Full Text] [PDF]