This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Yang, P.-C. Articles by Perdue, M. H. Search for Related Content PubMed PubMed Citation Articles by Yang, P.-C. Articles by Perdue, M. H.

(American Journal of Pathology. 2001;158:681-690.)
© 2001 American Society for Investigative Pathology

Regular Article

Mucosal Pathophysiology and Inflammatory Changes in the Late Phase of the Intestinal Allergic Reaction in the Rat

From the Intestinal Disease Research Program, McMaster University, Hamilton, Ontario, Canada

Relatively little information exists concerning the late phase of the allergic reaction in the gastrointestinal tract. Here, we characterized jejunal mucosal pathophysiology and inflammation after oral antigen challenge of sensitized rats, and examined the role of mast cells in events after challenge. Sprague-Dawley rats, mast cell-deficient (Ws/Ws), and +/+ control rats were sensitized to horseradish peroxidase, and challenged intragastrically with antigen 14 days later. Jejunal segments were obtained at 0.5 to 72 hours after challenge for functional assessment in Ussing chambers and for morphological assessment by light and electron microscopy. Intestine from sensitized Sprague-Dawley rats demonstrated enhanced ion secretion and permeability at all times after challenge. Electron microscopy revealed abnormal mitochondria within enterocytes and disruption of the epithelial basement membrane associated with influx into the mucosa of mast cells, eosinophils, neutrophils, and mononuclear cells. Many inflammatory cells appeared activated. In contrast, antigen-challenged Ws/Ws rats demonstrated no functional changes or inflammatory cell infiltrate. We conclude that oral antigen challenge of sensitized rats induces sustained epithelial dysfunction. Mast cells mediate both epithelial pathophysiology and recruitment of additional inflammatory cells that may contribute to persistent pathophysiology and symptoms.

This article has been cited by other articles:

				R G Nielsen, C Fenger, C Bindslev-Jensen, and S Husby Eosinophilia in the upper gastrointestinal tract is not a characteristic feature in cow's milk sensitive gastro-oesophageal reflux disease. Measurement by two methodologies J. Clin. Pathol., January 1, 2006; 59(1): 89 - 94. [Abstract] [Full Text] [PDF]

				P.-C. Yang, J. Jury, J. D. Soderholm, P. M. Sherman, D. M. McKay, and M. H. Perdue Chronic Psychological Stress in Rats Induces Intestinal Sensitization to Luminal Antigens Am. J. Pathol., January 1, 2006; 168(1): 104 - 114. [Abstract] [Full Text] [PDF]

				G. T. Furuta, E. E. S. Nieuwenhuis, J. Karhausen, G. Gleich, R. S. Blumberg, J. J. Lee, and S. J. Ackerman Eosinophils alter colonic epithelial barrier function: role for major basic protein Am J Physiol Gastrointest Liver Physiol, November 1, 2005; 289(5): G890 - G897. [Abstract] [Full Text] [PDF]

				M. Boerma, J. Wang, J. Wondergem, J. Joseph, X. Qiu, R. H. Kennedy, and M. Hauer-Jensen Influence of Mast Cells on Structural and Functional Manifestations of Radiation-Induced Heart Disease Cancer Res., April 15, 2005; 65(8): 3100 - 3107. [Abstract] [Full Text] [PDF]

				T. Ogawa, S. Miura, Y. Tsuzuki, T. Ogino, K. Teramoto, T. Inamura, C. Watanabe, R. Hokari, H. Nagata, and H. Ishii Chronic allergy to dietary ovalbumin induces lymphocyte migration to rat small intestinal mucosa that is inhibited by MAdCAM-1 Am J Physiol Gastrointest Liver Physiol, May 1, 2004; 286(5): G702 - G710. [Abstract] [Full Text] [PDF]

				D. C. Cara, K. V. J. Ebbert, and D.-M. McCafferty Mast Cell-Independent Mechanisms of Immediate Hypersensitivity: A Role for Platelets J. Immunol., April 15, 2004; 172(8): 4964 - 4971. [Abstract] [Full Text] [PDF]

				V. S. OSSOVSKAYA and N. W. BUNNETT Protease-Activated Receptors: Contribution to Physiology and Disease Physiol Rev, April 1, 2004; 84(2): 579 - 621. [Abstract] [Full Text] [PDF]

				H. L. Cameron, P.-C. Yang, and M. H. Perdue Glucagon-like peptide-2-enhanced barrier function reduces pathophysiology in a model of food allergy Am J Physiol Gastrointest Liver Physiol, June 1, 2003; 284(6): G905 - G912. [Abstract] [Full Text] [PDF]

				D. R. S. Steiner, N. C. Gonzalez, and J. G. Wood Mast cells mediate the microvascular inflammatory response to systemic hypoxia J Appl Physiol, January 1, 2003; 94(1): 325 - 334. [Abstract] [Full Text] [PDF]

				R. Schramm, T. Schaefer, M. D. Menger, and H. Thorlacius Acute mast cell-dependent neutrophil recruitment in the skin is mediated by KC and LFA-1: inhibitory mechanisms of dexamethasone J. Leukoc. Biol., December 1, 2002; 72(6): 1122 - 1132. [Abstract] [Full Text] [PDF]