This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Katou, F. Articles by Motegi, K. Search for Related Content PubMed PubMed Citation Articles by Katou, F. Articles by Motegi, K.

(American Journal of Pathology. 2001;158:1263-1270.)
© 2001 American Society for Investigative Pathology

Regular Articles

Macrophage-Derived Chemokine (MDC/CCL22) and CCR4 Are Involved in the Formation of T Lymphocyte-Dendritic Cell Clusters in Human Inflamed Skin and Secondary Lymphoid Tissue

Fuminori Katou^*, Haruo Ohtani, Takashi Nakayama, Katsuhiko Ono, Kouji Matsushima^§, Anne Saaristo^¶, Hiroshi Nagura, Osamu Yoshie and Katsutoshi Motegi^*

From the Departments of Oral and Maxillofacial Surgery I,^*
and Pathology,
Tohoku University, Sendai, Japan; the Department of Bacteriology,
Kinki University, Osaka-Sayama, Japan; the Department of Molecular Preventive Medicine,^§
University of Tokyo, Tokyo, Japan; and the Molecular/Cancer Biology Laboratory and Department of Pathology,^¶
Haartman Institute, University of Helsinki, Helsinki, Finland

Our previous study demonstrated formation of T cell-dendritic cell (DC) clusters in inflamed dermis of intraorally autotransplanted skin flaps. Such T cell-DC clusters are supposed to be important for close interactions between T cells and DCs including the specific antigen presentation. Here we show the involvement of the macrophage-derived chemokine (MDC/CCL22) and its specific receptor CC chemokine receptor 4 (CCR4) in the formation of T cell-DC clusters. Reverse transcriptase-polymerase chain reaction analysis revealed high levels of mRNA expression for MDC and CCR4 in inflamed skin and neck lymph nodes (LNs), but not in normal skin. Immunohistochemically, MDC⁺ cells and CCR4⁺ cells were mainly located within the T cell-DC clusters both in the dermis of inflamed skin and the T cell area of LNs. MDC⁺ cells were identified to be DCs both in inflamed skin and LNs. The majority of CCR4⁺ cells were CD4⁺ T cells, accounting for approximately one-third of total CD4⁺ T cells in the inflamed skin. Our data suggest that the MDC-CCR4 system plays an important role in the formation of T cell-DC clusters both in inflamed skin and LNs.

This article has been cited by other articles:

				J. Bayry, E. Z. Tchilian, M. N. Davies, E. K. Forbes, S. J. Draper, S. V. Kaveri, A. V. S. Hill, M. D. Kazatchkine, P. C. L. Beverley, D. R. Flower, et al. In silico identified CCR4 antagonists target regulatory T cells and exert adjuvant activity in vaccination PNAS, July 22, 2008; 105(29): 10221 - 10226. [Abstract] [Full Text] [PDF]

				F. Katou, H. Ohtani, Y. Watanabe, T. Nakayama, O. Yoshie, and K. Hashimoto Differing Phenotypes between Intraepithelial and Stromal Lymphocytes in Early-Stage Tongue Cancer Cancer Res., December 1, 2007; 67(23): 11195 - 11201. [Abstract] [Full Text] [PDF]

				B. D. Sather, P. Treuting, N. Perdue, M. Miazgowicz, J. D. Fontenot, A. Y. Rudensky, and D. J. Campbell Altering the distribution of Foxp3+ regulatory T cells results in tissue-specific inflammatory disease J. Exp. Med., June 11, 2007; 204(6): 1335 - 1347. [Abstract] [Full Text] [PDF]

				M. Bogunovic, F. Ginhoux, A. Wagers, M. Loubeau, L. M. Isola, L. Lubrano, V. Najfeld, R. G. Phelps, C. Grosskreutz, E. Scigliano, et al. Identification of a radio-resistant and cycling dermal dendritic cell population in mice and men J. Exp. Med., November 27, 2006; 203(12): 2627 - 2638. [Abstract] [Full Text] [PDF]

				C. M. Freeman, V. R. Stolberg, B.-C. Chiu, N. W. Lukacs, S. L. Kunkel, and S. W. Chensue CCR4 Participation in Th Type 1 (Mycobacterial) and Th Type 2 (Schistosomal) Anamnestic Pulmonary Granulomatous Responses J. Immunol., September 15, 2006; 177(6): 4149 - 4158. [Abstract] [Full Text] [PDF]

				A. Dongari-Bagtzoglou and P.L. Fidel Jr. The Host Cytokine Responses and Protective Immunity in Oropharyngeal Candidiasis J. Dent. Res., November 1, 2005; 84(11): 966 - 977. [Abstract] [Full Text] [PDF]

				R. Bonecchi, M. Locati, E. Galliera, M. Vulcano, M. Sironi, A. M. Fra, M. Gobbi, A. Vecchi, S. Sozzani, B. Haribabu, et al. Differential Recognition and Scavenging of Native and Truncated Macrophage-Derived Chemokine (Macrophage-Derived Chemokine/CC Chemokine Ligand 22) by the D6 Decoy Receptor J. Immunol., April 15, 2004; 172(8): 4972 - 4976. [Abstract] [Full Text] [PDF]

				B. Chazaud, C. Sonnet, P. Lafuste, G. Bassez, A.-C. Rimaniol, F. Poron, F.-J. Authier, P. A. Dreyfus, and R. K. Gherardi Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth J. Cell Biol., December 8, 2003; 163(5): 1133 - 1143. [Abstract] [Full Text] [PDF]

				R. Jotwani and C.W. Cutler Multiple Dendritic Cell (DC) Subpopulations in Human Gingiva and Association of Mature DCs with CD4+ T-cells in situ J. Dent. Res., September 1, 2003; 82(9): 736 - 741. [Abstract] [Full Text] [PDF]

				W. Iijima, H. Ohtani, T. Nakayama, Y. Sugawara, E. Sato, H. Nagura, O. Yoshie, and T. Sasano Infiltrating CD8+ T Cells in Oral Lichen Planus Predominantly Express CCR5 and CXCR3 and Carry Respective Chemokine Ligands RANTES/CCL5 and IP-10/CXCL10 in Their Cytolytic Granules: A Potential Self-Recruiting Mechanism Am. J. Pathol., July 1, 2003; 163(1): 261 - 268. [Abstract] [Full Text] [PDF]

				K. Tsumori, H. Kohrogi, E. Goto, N. Hirata, S. Hirosako, K. Fujii, M. Ando, O. Kawano, and H. Mizuta T Cells of Atopic Asthmatics Preferentially Infiltrate Into Human Bronchial Xenografts in SCID Mice J. Immunol., June 1, 2003; 170(11): 5712 - 5718. [Abstract] [Full Text] [PDF]

				M. Vulcano, S. Struyf, P. Scapini, M. Cassatella, S. Bernasconi, R. Bonecchi, A. Calleri, G. Penna, L. Adorini, W. Luini, et al. Unique Regulation of CCL18 Production by Maturing Dendritic Cells J. Immunol., April 1, 2003; 170(7): 3843 - 3849. [Abstract] [Full Text] [PDF]

				J. Alferink, I. Lieberam, W. Reindl, A. Behrens, S. Weiss, N. Huser, K. Gerauer, R. Ross, A. B. Reske-Kunz, P. Ahmad-Nejad, et al. Compartmentalized Production of CCL17 In Vivo: Strong Inducibility in Peripheral Dendritic Cells Contrasts Selective Absence from the Spleen J. Exp. Med., March 3, 2003; 197(5): 585 - 599. [Abstract] [Full Text] [PDF]

				K. Hieshima, H. Ohtani, M. Shibano, D. Izawa, T. Nakayama, Y. Kawasaki, F. Shiba, M. Shiota, F. Katou, T. Saito, et al. CCL28 Has Dual Roles in Mucosal Immunity as a Chemokine with Broad-Spectrum Antimicrobial Activity J. Immunol., February 1, 2003; 170(3): 1452 - 1461. [Abstract] [Full Text] [PDF]

				T. Horikawa, T. Nakayama, I. Hikita, H. Yamada, R. Fujisawa, T. Bito, S. Harada, A. Fukunaga, D. Chantry, P. W. Gray, et al. IFN-{gamma}-inducible expression of thymus and activation-regulated chemokine/CCL17 and macrophage-derived chemokine/CCL22 in epidermal keratinocytes and their roles in atopic dermatitis Int. Immunol., July 1, 2002; 14(7): 767 - 773. [Abstract] [Full Text] [PDF]

				O. Yoshie, R. Fujisawa, T. Nakayama, H. Harasawa, H. Tago, D. Izawa, K. Hieshima, Y. Tatsumi, K. Matsushima, H. Hasegawa, et al. Frequent expression of CCR4 in adult T-cell leukemia and human T-cell leukemia virus type 1-transformed T cells Blood, March 1, 2002; 99(5): 1505 - 1511. [Abstract] [Full Text] [PDF]