| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Regular Articles |
From the Lombardi Cancer Center, Georgetown University Medical Center, Washington, District of Columbia
Matriptase and its cognate, Kunitz-type serine protease inhibitor, HAI-1, comprise a newly characterized extracellular matrix-degrading protease system that may function as an epithelial membrane activator for other proteases and latent growth factors. Both enzyme and inhibitor have been detected in breast cancer cells, immortalized mammary epithelial cells, and human milk, but not in cultured fibroblasts nor in fibrosarcoma cells. To test the hypothesis that this system is expressed by normal breast epithelium, invasive breast cancers, and other cancers of an epithelial origin (carcinomas) but not in cancers of a mesenchymal origin, we have expanded our expression analysis of matriptase and HAI-1 in vitro and in vivo. Matriptase and HAI-1 were detected at the protein and mRNA levels both in hormone-dependent and hormone-independent cultured breast cancer cells, and this expression correlated with the expression of the epithelial markers E-cadherin or ZO-1. However, none of the breast cancer cell lines tested that express the mesenchymal marker vimentin express matriptase or HAI-1, consistent with an epithelial-selective expression of this system. Expression of matriptase, as determined by Western blot analysis, was observed in primary human breast, gynecological, and colon carcinomas, but not in stromal-derived ovarian tumors and human sarcomas of various origins and histological grades. The epithelial-selective expression of matriptase and HAI-1 was further confirmed in human breast cancers by immunohistochemistry and in situ hybridization, where the expression of the protease and the inhibitor were found in the carcinoma cells and in surrounding normal breast epithelia. The expression of the matriptase/HAI-1 system by malignant epithelial cells in vivo suggests a possible role for this protease in multiple aspects of the pathophysiology of epithelial malignancy, including invasion and metastasis.
This article has been cited by other articles:
 |
|
 |
|
  R. Szabo, P. Kosa, K. List, and T. H. Bugge Loss of Matriptase Suppression Underlies Spint1 Mutation-Associated Ichthyosis and Postnatal Lethality Am. J. Pathol., June 1, 2009; 174(6): 2015 - 2022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Cheng, T. Fukushima, N. Takahashi, H. Tanaka, and H. Kataoka Hepatocyte Growth Factor Activator Inhibitor Type 1 Regulates Epithelial to Mesenchymal Transition through Membrane-Bound Serine Proteinases Cancer Res., March 1, 2009; 69(5): 1828 - 1835. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Janciauskiene, I. Nita, D. Subramaniyam, Q. Li, J. R. Lancaster Jr., and S. Matalon {alpha}1-Antitrypsin Inhibits the Activity of the Matriptase Catalytic Domain In Vitro Am. J. Respir. Cell Mol. Biol., December 1, 2008; 39(6): 631 - 637. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Nagaike, M. Kawaguchi, N. Takeda, T. Fukushima, A. Sawaguchi, K. Kohama, M. Setoyama, and H. Kataoka Defect of Hepatocyte Growth Factor Activator Inhibitor Type 1/Serine Protease Inhibitor, Kunitz Type 1 (Hai-1/Spint1) Leads to Ichthyosis-Like Condition and Abnormal Hair Development in Mice Am. J. Pathol., November 1, 2008; 173(5): 1464 - 1475. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I-C. Tseng, F.-P. Chou, S.-F. Su, M. Oberst, N. Madayiputhiya, M.-S. Lee, J.-K. Wang, D. E. Sloane, M. Johnson, and C.-Y. Lin Purification from human milk of matriptase complexes with secreted serpins: mechanism for inhibition of matriptase other than HAI-1 Am J Physiol Cell Physiol, August 1, 2008; 295(2): C423 - C431. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, S. Wen, N. W. Bunnett, R. Leduc, M. D. Hollenberg, and W. K. MacNaughton Proteinase-activated Receptor-2 Induces Cyclooxygenase-2 Expression through -Catenin and Cyclic AMP-response Element-binding Protein J. Biol. Chem., January 11, 2008; 283(2): 809 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Mathias, M. E. Dodd, K. B. Walters, J. Rhodes, J. P. Kanki, A. T. Look, and A. Huttenlocher Live imaging of chronic inflammation caused by mutation of zebrafish Hai1 J. Cell Sci., October 1, 2007; 120(19): 3372 - 3383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Carney, S. von der Hardt, C. Sonntag, A. Amsterdam, J. Topczewski, N. Hopkins, and M. Hammerschmidt Inactivation of serine protease Matriptase1a by its inhibitor Hai1 is required for epithelial integrity of the zebrafish epidermis Development, October 1, 2007; 134(19): 3461 - 3471. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.-S. Lee, I-C. Tseng, Y. Wang, K.-i. Kiyomiya, M. D. Johnson, R. B. Dickson, and C.-Y. Lin Autoactivation of matriptase in vitro: requirement for biomembrane and LDL receptor domain Am J Physiol Cell Physiol, July 1, 2007; 293(1): C95 - C105. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Parr, A. J. Sanders, G. Davies, T. Martin, J. Lane, M. D. Mason, R. E. Mansel, and W. G. Jiang Matriptase-2 Inhibits Breast Tumor Growth and Invasion and Correlates with Favorable Prognosis for Breast Cancer Patients Clin. Cancer Res., June 15, 2007; 13(12): 3568 - 3576. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Netzel-Arnett, B. M. Currie, R. Szabo, C.-Y. Lin, L.-M. Chen, K. X. Chai, T. M. Antalis, T. H. Bugge, and K. List Evidence for a Matriptase-Prostasin Proteolytic Cascade Regulating Terminal Epidermal Differentiation J. Biol. Chem., November 3, 2006; 281(44): 32941 - 32945. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Kilpatrick, R. L. Harris, K. A. Owen, R. Bass, C. Ghorayeb, A. Bar-Or, and V. Ellis Initiation of plasminogen activation on the surface of monocytes expressing the type II transmembrane serine protease matriptase Blood, October 15, 2006; 108(8): 2616 - 2623. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. K. Hallikas, J. M. Aaltonen, H. von Koskull, L.-A. Lindberg, L. Valmu, N. Kalkkinen, T. Wahlstrom, H. Kataoka, L. Andersson, D. Lindholm, et al. Identification of Antibodies against HAI-1 and Integrin {alpha}6{beta}4 as Immunohistochemical Markers of Human Villous Cytotrophoblast J. Histochem. Cytochem., July 1, 2006; 54(7): 745 - 752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-i. Kiyomiya, M.-S. Lee, I-C. Tseng, H. Zuo, R. J. Barndt, M. D. Johnson, R. B. Dickson, and C.-Y. Lin Matriptase activation and shedding with HAI-1 is induced by steroid sex hormones in human prostate cancer cells, but not in breast cancer cells Am J Physiol Cell Physiol, July 1, 2006; 291(1): C40 - C49. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Le Naour, M. Andre, C. Greco, M. Billard, B. Sordat, J.-F. Emile, F. Lanza, C. Boucheix, and E. Rubinstein Profiling of the Tetraspanin Web of Human Colon Cancer Cells Mol. Cell. Proteomics, May 1, 2006; 5(5): 845 - 857. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ito, A. Akinaga, K. Yamanaka, T. Nakagawa, A. Kondo, R. B. Dickson, C.-Y. Lin, A. Miyauchi, N. Taniguchi, and E. Miyoshi Co-expression of matriptase and N-acetylglucosaminyltransferase V in thyroid cancer tissues--its possible role in prolonged stability in vivo by aberrant glycosylation Glycobiology, May 1, 2006; 16(5): 368 - 374. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. List, R. Szabo, A. Molinolo, B. S. Nielsen, and T. H. Bugge Delineation of Matriptase Protein Expression by Enzymatic Gene Trapping Suggests Diverging Roles in Barrier Function, Hair Formation, and Squamous Cell Carcinogenesis Am. J. Pathol., May 1, 2006; 168(5): 1513 - 1525. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Ge, H. Hu, K. Ding, L. Sun, and S. Zheng Protein Interaction Analysis of ST14 Domains and Their Point and Deletion Mutants J. Biol. Chem., March 17, 2006; 281(11): 7406 - 7412. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Saleem, V. M. Adhami, W. Zhong, B. J. Longley, C.-Y. Lin, R. B. Dickson, S. Reagan-Shaw, D. F. Jarrard, and H. Mukhtar A novel biomarker for staging human prostate adenocarcinoma: overexpression of matriptase with concomitant loss of its inhibitor, hepatocyte growth factor activator inhibitor-1. Cancer Epidemiol. Biomarkers Prev., February 1, 2006; 15(2): 217 - 227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-S. Jin, A. Chen, D.-S. Hsieh, C.-W. Yao, M.-F. Cheng, and Y.-F. Lin Expression of Serine Protease Matriptase in Renal Cell Carcinoma: Correlation of Tissue Microarray Immunohistochemical Expression Analysis Results with Clinicopathological Parameters International Journal of Surgical Pathology, January 1, 2006; 14(1): 65 - 72. [Abstract] [PDF]
|
|
|
|
 |
|
 K. List, R. Szabo, A. Molinolo, V. Sriuranpong, V. Redeye, T. Murdock, B. Burke, B. S. Nielsen, J. S. Gutkind, and T. H. Bugge Deregulated matriptase causes ras-independent multistage carcinogenesis and promotes ras-mediated malignant transformation Genes & Dev., August 15, 2005; 19(16): 1934 - 1950. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Oberst, L.-Y. L. Chen, K.-I. Kiyomiya, C. A. Williams, M.-S. Lee, M. D. Johnson, R. B. Dickson, and C.-Y. Lin HAI-1 regulates activation and expression of matriptase, a membrane-bound serine protease Am J Physiol Cell Physiol, August 1, 2005; 289(2): C462 - C470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. S. Knudsen, J. M. Lucas, L. Fazli, S. Hawley, S. Falcon, I. M. Coleman, D. B. Martin, C. Xu, L. D. True, M. E. Gleave, et al. Regulation of Hepatocyte Activator Inhibitor-1 Expression by Androgen and Oncogenic Transformation in the Prostate Am. J. Pathol., July 1, 2005; 167(1): 255 - 266. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. X. Pedersen, C. J. Pennington, K. Almholt, I. J. Christensen, B. S. Nielsen, D. R. Edwards, J. Romer, K. Dano, and M. Johnsen Extracellular protease mRNAs are predominantly expressed in the stromal areas of microdissected mouse breast carcinomas Carcinogenesis, July 1, 2005; 26(7): 1233 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M Lacroix, R-A Toillon, and G Leclercq Stable 'portrait' of breast tumors during progression: data from biology, pathology and genetics Endocr. Relat. Cancer, September 1, 2004; 11(3): 497 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Noel, C Maillard, N Rocks, M Jost, V Chabottaux, N E Sounni, E Maquoi, D Cataldo, and J M Foidart Membrane associated proteases and their inhibitors in tumour angiogenesis J. Clin. Pathol., June 1, 2004; 57(6): 577 - 584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. U. Di Maria and P. Comba Malignant Pleural Mesothelioma: The Puzzling Role of Gene-Environment Interaction Chest, May 1, 2004; 125(5): 1604 - 1607. [Full Text] [PDF]
|
|
|
|
|
|
C. D. Hoang, J. D'Cunha, M. G. Kratzke, C. E. Casmey, S. P. Frizelle, M. A. Maddaus, and R. A. Kratzke Gene Expression Profiling Identifies Matriptase Overexpression in Malignant Mesothelioma Chest, May 1, 2004; 125(5): 1843 - 1852. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.-J. Hung, I.-W. J. Hsu, J. L. Dreiling, M.-J. Lee, C. A. Williams, M. D. Oberst, R. B. Dickson, and C.-Y. Lin Assembly of adherens junctions is required for sphingosine 1-phosphate-induced matriptase accumulation and activation at mammary epithelial cell-cell contacts Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1159 - C1169. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Suzuki, H. Kobayashi, N. Kanayama, Y. Saga, M. Suzuki, C.-Y. Lin, R. B. Dickson, and T. Terao Inhibition of Tumor Invasion by Genomic Down-regulation of Matriptase through Suppression of Activation of Receptor-bound Pro-urokinase J. Biol. Chem., April 9, 2004; 279(15): 14899 - 14908. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. B. Martin, D. R. Gifford, M. E. Wright, A. Keller, E. Yi, D. R. Goodlett, R. Aebersold, and P. S. Nelson Quantitative Proteomic Analysis of Proteins Released by Neoplastic Prostate Epithelium Cancer Res., January 1, 2004; 64(1): 347 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Parr, G. Watkins, R. E. Mansel, and W. G. Jiang The Hepatocyte Growth Factor Regulatory Factors in Human Breast Cancer Clin. Cancer Res., January 1, 2004; 10(1): 202 - 211. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. List, R. Szabo, P. W. Wertz, J. Segre, C. C. Haudenschild, S.-Y. Kim, and T. H. Bugge Loss of proteolytically processed filaggrin caused by epidermal deletion of Matriptase/MT-SP1 J. Cell Biol., November 24, 2003; 163(4): 901 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kirchhofer, M. Peek, W. Li, J. Stamos, C. Eigenbrot, S. Kadkhodayan, J. M. Elliott, R. T. Corpuz, R. A. Lazarus, and P. Moran Tissue Expression, Protease Specificity, and Kunitz Domain Functions of Hepatocyte Growth Factor Activator Inhibitor-1B (HAI-1B), a New Splice Variant of HAI-1 J. Biol. Chem., September 19, 2003; 278(38): 36341 - 36349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Oberst, B. Singh, M. Ozdemirli, R. B. Dickson, M. D. Johnson, and C.-Y. Lin Characterization of Matriptase Expression in Normal Human Tissues J. Histochem. Cytochem., August 1, 2003; 51(8): 1017 - 1025. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Oberst, C. A. Williams, R. B. Dickson, M. D. Johnson, and C.-Y. Lin The Activation of Matriptase Requires Its Noncatalytic Domains, Serine Protease Domain, and Its Cognate Inhibitor J. Biol. Chem., July 11, 2003; 278(29): 26773 - 26779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Y. Kang, M. Dolled-Filhart, I. T. Ocal, B. Singh, C.-Y. Lin, R. B. Dickson, D. L. Rimm, and R. L. Camp Tissue Microarray Analysis of Hepatocyte Growth Factor/Met Pathway Components Reveals a Role for Met, Matriptase, and Hepatocyte Growth Factor Activator Inhibitor 1 in the Progression of Node-negative Breast Cancer Cancer Res., March 1, 2003; 63(5): 1101 - 1105. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Velasco, S. Cal, V. Quesada, L. M. Sanchez, and C. Lopez-Otin Matriptase-2, a Membrane-bound Mosaic Serine Proteinase Predominantly Expressed in Human Liver and Showing Degrading Activity against Extracellular Matrix Proteins J. Biol. Chem., September 27, 2002; 277(40): 37637 - 37646. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Hollenberg and S. J. Compton International Union of Pharmacology. XXVIII. Proteinase-Activated Receptors Pharmacol. Rev., June 1, 2002; 54(2): 203 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Oberst, M. D. Johnson, R. B. Dickson, C.-Y. Lin, B. Singh, M. Stewart, A. Williams, A. al-Nafussi, J. F. Smyth, H. Gabra, et al. Expression of the Serine Protease Matriptase and Its Inhibitor HAI-1 in Epithelial Ovarian Cancer: Correlation with Clinical Outcome and Tumor Clinicopathological Parameters Clin. Cancer Res., April 1, 2002; 8(4): 1101 - 1107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Benaud, M. Oberst, J. P. Hobson, S. Spiegel, R. B. Dickson, and C.-Y. Lin Sphingosine 1-Phosphate, Present in Serum-derived Lipoproteins, Activates Matriptase J. Biol. Chem., March 15, 2002; 277(12): 10539 - 10546. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Friedrich, P. Fuentes-Prior, E. Ong, G. Coombs, M. Hunter, R. Oehler, D. Pierson, R. Gonzalez, R. Huber, W. Bode, et al. Catalytic Domain Structures of MT-SP1/Matriptase, a Matrix-degrading Transmembrane Serine Proteinase J. Biol. Chem., January 11, 2002; 277(3): 2160 - 2168. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
