help button home button Am J Pathol ASIP WHAT IS IT?
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow A correction has been published
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Young, A. N.
Right arrow Articles by Neish, A. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Young, A. N.
Right arrow Articles by Neish, A. S.
(American Journal of Pathology. 2001;158:1639-1651.)
© 2001 American Society for Investigative Pathology

Regular Articles

Expression Profiling of Renal Epithelial Neoplasms

A Method for Tumor Classification and Discovery of Diagnostic Molecular Markers

Andrew N. Young*§, Mahul B. Amin*{dagger}, Carlos S. Moreno{ddagger}, So Dug Lim*, Cynthia Cohen*, John A. Petros{dagger}§, Fray F. Marshall{dagger} and Andrew S. Neish*

From the Departments of Pathology and Laboratory Medicine,*
Urology,{dagger}
and Biochemistry,{ddagger}
Emory University School of Medicine, Atlanta; and the Atlanta VA Medical Center,§
Decatur, Georgia

The expression patterns of 7075 genes were analyzed in four conventional (clear cell) renal cell carcinomas (RCC), one chromophobe RCC, and two oncocytomas using cDNA microarrays. Expression profiles were compared among tumors using various clustering algorithms, thereby separating the tumors into two categories consistent with corresponding histopathological diagnoses. Specifically, conventional RCCs were distinguished from chromophobe RCC/oncocytomas based on large-scale gene expression patterns. Chromophobe RCC/oncocytomas displayed similar expression profiles, including genes involved with oxidative phosphorylation and genes expressed normally by distal nephron, consistent with the mitochondrion-rich morphology of these tumors and the theory that both lesions are related histogenetically to distal nephron epithelium. Conventional RCCs underexpressed mitochondrial and distal nephron genes, and were further distinguished from chromophobe RCC/oncocytomas by overexpression of vimentin and class II major histocompatibility complex-related molecules. Novel, tumor-specific expression of four genes—vimentin, class II major histocompatibility complex-associated invariant chain (CD74), parvalbumin, and galectin-3—was confirmed in an independent tumor series by immunohistochemistry. Vimentin was a sensitive, specific marker for conventional RCCs, and parvalbumin was detected primarily in chromophobe RCC/oncocytomas. In conclusion, histopathological subtypes of renal epithelial neoplasia were characterized by distinct patterns of gene expression. Expression patterns were useful for identifying novel molecular markers with potential diagnostic utility.




This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
Y. Gore, D. Starlets, N. Maharshak, S. Becker-Herman, U. Kaneyuki, L. Leng, R. Bucala, and I. Shachar
Macrophage Migration Inhibitory Factor Induces B Cell Survival by Activation of a CD74-CD44 Receptor Complex
J. Biol. Chem., February 1, 2008; 283(5): 2784 - 2792.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
S. Rohan, J. J. Tu, J. Kao, P. Mukherjee, F. Campagne, X. K. Zhou, E. Hyjek, M. A. Alonso, and Y.-T. Chen
Gene Expression Profiling Separates Chromophobe Renal Cell Carcinoma from Oncocytoma and Identifies Vesicular Transport and Cell Junction Proteins as Differentially Expressed Genes
Clin. Cancer Res., December 1, 2006; 12(23): 6937 - 6945.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
K. Hotakainen, S. Lintula, B. Ljungberg, P. Finne, A. Paju, U.-H. Stenman, and J. Stenman
Expression Of Human Chorionic Gonadotropin {beta}-Subunit Type I Genes Predicts Adverse Outcome In Renal Cell Carcinoma
J. Mol. Diagn., November 1, 2006; 8(5): 598 - 603.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
D. Starlets, Y. Gore, I. Binsky, M. Haran, N. Harpaz, L. Shvidel, S. Becker-Herman, A. Berrebi, and I. Shachar
Cell-surface CD74 initiates a signaling cascade leading to cell proliferation and survival
Blood, June 15, 2006; 107(12): 4807 - 4816.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
J. D. Raman, N. P. Mongan, L. Liu, S. K. Tickoo, D. M. Nanus, D. S. Scherr, and L. J. Gudas
Decreased expression of the human stem cell marker, Rex-1 (zfp-42), in renal cell carcinoma
Carcinogenesis, March 1, 2006; 27(3): 499 - 507.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
Y.-T. Chen, J. J. Tu, J. Kao, X. K. Zhou, and M. Mazumdar
Messenger RNA Expression Ratios among Four Genes Predict Subtypes of Renal Cell Carcinoma and Distinguish Oncocytoma from Carcinoma
Clin. Cancer Res., September 15, 2005; 11(18): 6558 - 6566.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
X. J. Yang, M.-H. Tan, H. L. Kim, J. A. Ditlev, M. W. Betten, C. E. Png, E. J. Kort, K. Futami, K. A. Furge, M. Takahashi, et al.
A Molecular Classification of Papillary Renal Cell Carcinoma
Cancer Res., July 1, 2005; 65(13): 5628 - 5637.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. Togashi, T. Katagiri, S. Ashida, T. Fujioka, O. Maruyama, Y. Wakumoto, Y. Sakamoto, M. Fujime, Y. Kawachi, T. Shuin, et al.
Hypoxia-Inducible Protein 2 (HIG2), a Novel Diagnostic Marker for Renal Cell Carcinoma and Potential Target for Molecular Therapy
Cancer Res., June 1, 2005; 65(11): 4817 - 4826.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
A. N. Schuetz, Q. Yin-Goen, M. B. Amin, C. S. Moreno, C. Cohen, C. D. Hornsby, W. L. Yang, J. A. Petros, M. M. Issa, J. G. Pattaras, et al.
Molecular Classification of Renal Tumors by Gene Expression Profiling
J. Mol. Diagn., May 1, 2005; 7(2): 206 - 218.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
E. Wang, R. Lichtenfels, J. Bukur, Y. Ngalame, M. C. Panelli, B. Seliger, and F. M. Marincola
Ontogeny and Oncogenesis Balance the Transcriptional Profile of Renal Cell Cancer
Cancer Res., October 15, 2004; 64(20): 7279 - 7287.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M.-H. Tan, C. G. Rogers, J. T. Cooper, J. A. Ditlev, T. J. Maatman, X. Yang, K. A. Furge, and B. T. Teh
Gene Expression Profiling of Renal Cell Carcinoma
Clin. Cancer Res., September 15, 2004; 10(18): 6315S - 6321S.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
W. M. Stadler
Therapeutic Options for Variant Renal Cancer: A True Orphan Disease
Clin. Cancer Res., September 15, 2004; 10(18): 6393S - 6396S.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
K. Struckmann, P. Schraml, R. Simon, K. Elmenhorst, M. Mirlacher, J. Kononen, and H. Moch
Impaired Expression of the Cell Cycle Regulator BTG2 Is Common in Clear Cell Renal Cell Carcinoma
Cancer Res., March 1, 2004; 64(5): 1632 - 1638.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
G. Li, M. Cuilleron, A. Gentil-Perret, M. Cottier, K. Passebosc-Faure, C. Lambert, C. Genin, and J. Tostain
Rapid and Sensitive Detection of Messenger RNA Expression for Molecular Differential Diagnosis of Renal Cell Carcinoma
Clin. Cancer Res., December 15, 2003; 9(17): 6441 - 6446.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pathol.Home page
S D Jenson, R S Robetorye, S D Bohling, J A Schumacher, J W Morgan, M S Lim, and K S J Elenitoba-Johnson
Validation of cDNA microarray gene expression data obtained from linearly amplified RNA
Mol. Pathol., December 1, 2003; 56(6): 307 - 312.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
N. C. Twine, J. A. Stover, B. Marshall, G. Dukart, M. Hidalgo, W. Stadler, T. Logan, J. Dutcher, G. Hudes, A. J. Dorner, et al.
Disease-associated Expression Profiles in Peripheral Blood Mononuclear Cells from Patients with Advanced Renal Cell Carcinoma
Cancer Res., September 15, 2003; 63(18): 6069 - 6075.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
X. Zhang, I. H. Diab, and Z. E. Zehner
ZBP-89 represses vimentin gene transcription by interacting with the transcriptional activator, Sp1
Nucleic Acids Res., June 1, 2003; 31(11): 2900 - 2914.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. P.T. Higgins, R. Shinghal, H. Gill, J. H. Reese, M. Terris, R. J. Cohen, M. Fero, J. R. Pollack, M. van de Rijn, and J. D. Brooks
Gene Expression Patterns in Renal Cell Carcinoma Assessed by Complementary DNA Microarray
Am. J. Pathol., March 1, 2003; 162(3): 925 - 932.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
E. C. Ibrahim, Y. Allory, F. Commo, B. Gattegno, P. Callard, and P. Paul
Altered Pattern of Major Histocompatibility Complex Expression in Renal Carcinoma: Tumor-Specific Expression of the Nonclassical Human Leukocyte Antigen-G Molecule Is Restricted to Clear Cell Carcinoma While Up-Regulation of Other Major Histocompatibility Complex Antigens Is Primarily Distributed in All Subtypes of Renal Carcinoma
Am. J. Pathol., February 1, 2003; 162(2): 501 - 508.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
T. J. Giordano, D. G. Thomas, R. Kuick, M. Lizyness, D. E. Misek, A. L. Smith, D. Sanders, R. T. Aljundi, P. G. Gauger, N. W. Thompson, et al.
Distinct Transcriptional Profiles of Adrenocortical Tumors Uncovered by DNA Microarray Analysis
Am. J. Pathol., February 1, 2003; 162(2): 521 - 531.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
T. Weinschenk, C. Gouttefangeas, M. Schirle, F. Obermayr, S. Walter, O. Schoor, R. Kurek, W. Loeser, K.-H. Bichler, D. Wernet, et al.
Integrated Functional Genomics Approach for the Design of Patient-individual Antitumor Vaccines
Cancer Res., October 15, 2002; 62(20): 5818 - 5827.
[Abstract] [Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
R. S. Robetorye, S. D. Bohling, J. W. Morgan, G. C. Fillmore, M. S. Lim, and K. S. J. Elenitoba-Johnson
Microarray Analysis of B-Cell Lymphoma Cell Lines with the t(14;18)
J. Mol. Diagn., August 1, 2002; 4(3): 123 - 136.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
D. A. Kirschmann, E. A. Seftor, S. F. T. Fong, D. R. C. Nieva, C. M. Sullivan, E. M. Edwards, P. Sommer, K. Csiszar, and M. J. C. Hendrix
A Molecular Role for Lysyl Oxidase in Breast Cancer Invasion
Cancer Res., August 1, 2002; 62(15): 4478 - 4483.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
A. T. Gewirtz, L. S. Collier-Hyams, A. N. Young, T. Kucharzik, W. J. Guilford, J. F. Parkinson, I. R. Williams, A. S. Neish, and J. L. Madara
Lipoxin A4 Analogs Attenuate Induction of Intestinal Epithelial Proinflammatory Gene Expression and Reduce the Severity of Dextran Sodium Sulfate-Induced Colitis
J. Immunol., May 15, 2002; 168(10): 5260 - 5267.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
H. Simonnet, N. Alazard, K. Pfeiffer, C. Gallou, C. Beroud, J. Demont, R. Bouvier, H. Schagger, and C. Godinot
Low mitochondrial respiratory chain content correlates with tumor aggressiveness in renal cell carcinoma
Carcinogenesis, May 1, 2002; 23(5): 759 - 768.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the American Society for Investigative Pathology.