| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Technical Advance |
From the Institutes of Clinical Pathology*
and
Neurology,
University of Vienna, Vienna,
Austria; the Department of Biology,
University of Konstanz, Konstanz, Germany; and the Department of
Immunology and Cell Biology,
Forschungsinstitut Borstel, Borstel, Germany
Monoclonal antibody MIB-1 is a reliable tool for determining proliferating cells in human tissues, but does not react with the homologous mouse antigen and is therefore useless in experimental pathology using mice as model systems. Standard method for assessment of cellular proliferation in formalin-fixed, paraffin-embedded murine tissues is immunohistochemical detection of DNA synthesis using antibodies against exogenously injected 5-bromodeoxyuridine (BrdU), which is a tedious procedure and not useful for routine investigations. We tested monoclonal antibody MIB-5 and monoclonal and polyclonal anti-MCM3 antibodies as immunohistochemical proliferation markers for paraffin-embedded nonneoplastic and neoplastic tissues of wild-type and transgenic mice, compared to anti-BrdU immunostaining. Percentage of proliferating cells was determined with continuously decreasing antibody dilutions. Percentages of MIB-5 and anti-BrdU immunostained cells correlated strongly, as well as percentage of MIB-5-decorated cells and frequency of mitotic figures. Anti-MCM3 antibodies labeled significantly higher percentages of cells than anti-BrdU or MIB-5, and showed a linear decrease with increasing antibody dilutions. We conclude that MIB-5 detects reliably the cell growth fraction in formalin fixed, paraffin-embedded murine tissues, bypassing methodological drawbacks of BrdU. Anti-MCM3 antibodies are less useful for determination of proliferating cells although they might detect the fraction of cells remaining competent for proliferation.
This article has been cited by other articles:
 |
|
 |
|
  R. Hlushchuk, O. Riesterer, O. Baum, J. Wood, G. Gruber, M. Pruschy, and V. Djonov Tumor Recovery by Angiogenic Switch from Sprouting to Intussusceptive Angiogenesis after Treatment with PTK787/ZK222584 or Ionizing Radiation Am. J. Pathol., October 1, 2008; 173(4): 1173 - 1185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. K. Meyerholz, J. A. DeGraaff, J. M. Gallup, A. K. Olivier, and M. R. Ackermann Depletion of Alveolar Glycogen Corresponds With Immunohistochemical Development of CD208 Antigen Expression in Perinatal Lamb Lung J. Histochem. Cytochem., November 1, 2006; 54(11): 1247 - 1253. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Cassie, I. Koturbash, D. Hudson, M. Baker, Y. Ilnytskyy, R. Rodriguez-Juarez, E. Weber, and O. Kovalchuk Novel retinoblastoma binding protein RBBP9 modulates sex-specific radiation responses in vivo Carcinogenesis, March 1, 2006; 27(3): 465 - 474. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Narasimhaiah, A. Tuchman, S. L. Lin, and J. R. Naegele Oxidative Damage and Defective DNA Repair is Linked to Apoptosis of Migrating Neurons and Progenitors During Cerebral Cortex Development in Ku70-Deficient Mice Cereb Cortex, June 1, 2005; 15(6): 696 - 707. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Carvalho, H. Vala, C. Pinto, M. Pinho, and M. C. Peleteiro Immunohistochemical Studies of Epithelial Cell Proliferation and p53 Mutation in Bovine Ocular Squamous Cell Carcinoma Vet. Pathol., January 1, 2005; 42(1): 66 - 73. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Cooperman, R. Neely, D. T. Teachey, S. Grupp, and J. K. Choi Cell Division Rates of Primary Human Precursor B Cells in Culture Reflect In Vivo Rates Stem Cells, November 1, 2004; 22(6): 1111 - 1120. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Emmenegger, S. Man, Y. Shaked, G. Francia, J. W. Wong, D. J. Hicklin, and R. S. Kerbel A Comparative Analysis of Low-Dose Metronomic Cyclophosphamide Reveals Absent or Low-Grade Toxicity on Tissues Highly Sensitive to the Toxic Effects of Maximum Tolerated Dose Regimens Cancer Res., June 1, 2004; 64(11): 3994 - 4000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Muskhelishvili, J. R. Latendresse, R. L. Kodell, and E. B. Henderson Evaluation of Cell Proliferation in Rat Tissues with BrdU, PCNA, Ki-67(MIB-5) Immunohistochemistry and In Situ Hybridization for Histone mRNA J. Histochem. Cytochem., December 1, 2003; 51(12): 1681 - 1688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Vet. Pathol., September 1, 2001; 38(5): 567 - 568. [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
