This Article Full Text Full Text (PDF) A correction has been published Purchase Article View Shopping Cart Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ahmed, Z. Articles by Cuzner, M. L. Search for Related Content PubMed PubMed Citation Articles by Ahmed, Z. Articles by Cuzner, M. L.

(American Journal of Pathology. 2001;158:2127-2138.)
© 2001 American Society for Investigative Pathology

Regular Article

Myelin/Axonal Pathology in Interleukin-12 Induced Serial Relapses of Experimental Allergic Encephalomyelitis in the Lewis Rat

Zubair Ahmed^*, Djordje Gveric^*, Gareth Pryce^*, David Baker^*, John P. Leonard and M. Louise Cuzner^*

From the Neuroinflammation Group,^*
Department of Neurochemistry, Institute of Neurology, University College London, London, United Kingdom; and the Genetics Institute,
Andover, Massachusetts

Lewis rats, on recovery from monophasic clinical experimental allergic encephalomyelitis (EAE), can be induced to develop repeated paralytic relapses with a graded reduction in clinical severity following intraperitoneal administration of IL-12. By the time of the third relapse, the number and size of inflammatory cuffs in the spinal cord were reduced with the makeup of the cellular infiltrate shifting to a significantly increased number of B cells. Serum levels of myelin basic protein (MBP)-specific IgG1 and IgG2b were found to rise over time while MBP and MBP peptide-positive macrophages and microglia became evident in perivascular cuffs and in spinal cord parenchyma, indicative of myelin phagocytosis. Axonal death was observed in semithin and EM sections of spinal cord in third relapse animals in association with iNOS and tPA immunostaining throughout gray and white matter. These neurotoxic or excitotoxic agents may contribute to axonal damage directly or indirectly by activated microglia and macrophages, leading to limited damage of the axonal-myelin unit.

This article has been cited by other articles:

				M. J Nunez, J. Balboa, M. Rey-Mendez, J. Brenlla, M. Gonzalez-Peteiro, E. Rodrigo, and M. Freire-Garabal Effects of amphetamine and cocaine on the development of acute experimental allergic encephalomyelitis in Lewis rats Human and Experimental Toxicology, August 1, 2007; 26(8): 637 - 643. [Abstract] [PDF]

				E. East, D. Baker, G. Pryce, H. R. Lijnen, M. L. Cuzner, and D. Gveric A Role for the Plasminogen Activator System in Inflammation and Neurodegeneration in the Central Nervous System during Experimental Allergic Encephalomyelitis Am. J. Pathol., August 1, 2005; 167(2): 545 - 554. [Abstract] [Full Text] [PDF]

				A. J. GROOM, T. SMITH, and L. TURSKI Multiple Sclerosis and Glutamate Ann. N.Y. Acad. Sci., May 1, 2003; 993(1): 229 - 275. [Abstract] [Full Text] [PDF]

				H. P. M. Brok, M. van Meurs, E. Blezer, A. Schantz, D. Peritt, G. Treacy, J. D. Laman, J. Bauer, and B. A. 't Hart Prevention of Experimental Autoimmune Encephalomyelitis in Common Marmosets Using an Anti-IL-12p40 Monoclonal Antibody J. Immunol., December 1, 2002; 169(11): 6554 - 6563. [Abstract] [Full Text] [PDF]

				Z. Ahmed, A. I. Doward, G. Pryce, D. L. Taylor, J. M. Pocock, J. P. Leonard, D. Baker, and M. L. Cuzner A Role for Caspase-1 and -3 in the Pathology of Experimental Allergic Encephalomyelitis : Inflammation Versus Degeneration Am. J. Pathol., November 1, 2002; 161(5): 1577 - 1586. [Abstract] [Full Text] [PDF]

				T. B. Jones, D. M. Basso, A. Sodhi, J. Z. Pan, R. P. Hart, R. C. MacCallum, S. Lee, C. C. Whitacre, and P. G. Popovich Pathological CNS Autoimmune Disease Triggered by Traumatic Spinal Cord Injury: Implications for Autoimmune Vaccine Therapy J. Neurosci., April 1, 2002; 22(7): 2690 - 2700. [Abstract] [Full Text] [PDF]