help button home button Am J Pathol ASIP MEMBERSHIP
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gusev, Y.
Right arrow Articles by Wheeler, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gusev, Y.
Right arrow Articles by Wheeler, V.
(American Journal of Pathology. 2001;159:63-69.)
© 2001 American Society for Investigative Pathology

Technical Advance

Rolling Circle Amplification

A New Approach to Increase Sensitivity for Immunohistochemistry and Flow Cytometry

Yuriy Gusev, Jason Sparkowski, Arumugham Raghunathan, Harley Ferguson, Jr., Jane Montano, Nancy Bogdan, Barry Schweitzer, Steven Wiltshire, Stephen F. Kingsmore, Warren Maltzman and Vanessa Wheeler

From Molecular Staging Inc., New Haven, Connecticut

Immunohistochemistry is a method that can provide complementary diagnostic and prognostic information to morphological observations and soluble assays. Sensitivity, specificity, or requirements for arduous sample preparation or signal amplification procedures often limit the application of this approach to routine clinical specimens. Rolling circle amplification (RCA) generates a localized signal via an isothermal amplification of an oligonucleotide circle. The application of this approach to immunohistochemistry could extend the utility of these methods to include a more complete set of immunological and molecular probes. RCA-mediated signal amplification was successfully applied to the sensitive and specific detection of a variety of cell surface antigens (CD3, CD20, and epithelial membrane antigen) and intracellular molecules (vimentin and prostate-specific antigen) within a variety of routinely fixed specimens, as well as samples prepared for flow cytometry. RCA technology, which has an intrinsically wide dynamic range, is a robust and simple procedure that can provide a universal platform for the localization of a wide variety of molecules as a function of either antigenicity or nucleic acid sequence. The use of RCA in this way could enhance the use of markers of current interest as well as permit the integration of emerging information from genomics and proteomics intocell- and tissue-based analyses.




This article has been cited by other articles:


Home page
 Clin. Chem.Home page
A. Raghunathan, M. P. Sorette, H. R. Ferguson Jr, and S. P. Piccoli
Rolling Circle Amplification Technology as a Potential Tool in Detection and Monitoring of Cancer by Flow Cytometry
Clin. Chem., October 1, 2002; 48(10): 1853 - 1855.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the American Society for Investigative Pathology.