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Short Communications |
From the Divisions of Molecular Medicine*
and
Epidemiology and Prevention,
and the
Department of Pathology and Genetics,
Aichi
Cancer Center, Nagoya; and SRL, Inc.,
Tokyo, Japan
The diagnosis of mantle cell lymphoma (MCL) is particularly
important for clinical management because of a remarkable prognostic
difference between MCL and other types of B-cell lymphoma. In addition
to immunohistochemical analysis, we have established a 5'
exonuclease-based real-time reverse transcriptase-mediated quantitative
polymerase chain reaction (RQ-PCR) method to detect cyclin D1
overexpression for the diagnosis of MCL. The RQ-PCR could detect cyclin
D1 overexpression in all nine examined MCL cases, in contrast
genomic PCR detected t(11;14) in only two of nine cases. By RQ-PCR the
expression of G6PDH was significantly higher in myeloid leukemias
than those in B-cell lymphomas (P = 0.018).
As a result, cyclin D1/G6PDH ratio ranged from 0.78 to 12.4
(mean, 1.83) in MCL, exclusively higher than those in
other B-cell lymphoma (0.00009 
0.16) and myeloid leukemia
(0.00011 0.085). The high expression of cyclin D1 in certain
myeloid leukemias was identified to reflect their proliferative
activity and not to represent the oncogenic overexpression. The 95%
confidence interval of the cyclin D1/G6PDH ratio was 0.29 11.1
for MCL, 0.014 0.25 for other B-cell lymphomas and
0.000014 0.083 for myeloid leukemia, suggesting that a
cutoff value can be set at 0.25. The RQ-PCR of cyclin D1 is convenient
and especially useful for the diagnosis of MCL.
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