help button home button Am J Pathol Epitomics Buy 2 Antibodies Get 1 Free Special Offer
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Low, Q. E. H.
Right arrow Articles by DiPietro, L. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Low, Q. E. H.
Right arrow Articles by DiPietro, L. A.
(American Journal of Pathology. 2001;159:457-463.)
© 2001 American Society for Investigative Pathology

Short Communications

Wound Healing in MIP-1{alpha}-/- and MCP-1-/- Mice

Quentin E. H. Low*{dagger}, Iulia A. Drugea{dagger}{ddagger}, Lisa A. Duffner{dagger}§, Daniel G. Quinn*, Donald N. Cook, Barrett J. Rollins||, Elizabeth J. Kovacs{dagger}§** and Luisa A. DiPietro*{dagger}{ddagger}**

From the Department of Microbiology and Immunology,*
Burn and Shock Trauma Institute,{dagger}
the Departments of Surgery{ddagger}
and Cell Biology, Neurobiology, and Anatomy,§
Immunology and Aging Program,**
Loyola University Medical Center, Maywood, Illinois; the Department of Immunology,
Schering-Plough Research Institute, Kenilworth, New Jersey; and the Department of Adult Oncology,||
Dana-Farber Cancer Institute, Boston, Massachusetts

A salient feature of normal wound healing is the development and resolution of an acute inflammatory response. Although much is known about the function of inflammatory cells within wounds, little is known about the chemotactic and activation signals that influence this response. As the CC chemokines macrophage inflammatory protein-1{alpha} (MIP-1{alpha}) and monocyte chemotactic protein-1 (MCP-1) are abundant in acute wounds, wound repair was examined in MIP-1{alpha}-/- and MCP-1-/- mice. Surprisingly, wound re-epithelialization, angiogenesis, and collagen synthesis in MIP-1{alpha}-/- mice was nearly identical to wild-type controls. In contrast, MCP-1-/- mice displayed significantly delayed wound re-epithelialization, with the greatest delay at day 3 after injury (28 ± 5% versus 79 ± 14% re-epithelialization, P < 0.005). Wound angiogenesis was also delayed in MCP-1-/- mice, with a 48% reduction in capillary density at day 5 after injury. Collagen synthesis was impeded as well, with the wounds of MCP-1-/- mice containing significantly less hydroxyproline than those of control mice (25 ± 3 versus 50 ± 8 µg/wound at day 5, P < 0.0001). No change in the number of wound macrophages was observed in MCP-1-/- mice, suggesting that monocyte recruitment into wounds is independent of this chemokine. The data suggest that MCP-1 plays a critical role in healing wounds, most likely by influencing the effector state of macrophages and other cell types.




This article has been cited by other articles:


Home page
 J. Immunol.Home page
Y. Ishida, J.-L. Gao, and P. M. Murphy
Chemokine Receptor CX3CR1 Mediates Skin Wound Healing by Promoting Macrophage and Fibroblast Accumulation and Function
J. Immunol., January 1, 2008; 180(1): 569 - 579.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
K. T. Keylock, V. J. Vieira, M. A. Wallig, L. A. DiPietro, M. Schrementi, and J. A. Woods
Exercise accelerates cutaneous wound healing and decreases wound inflammation in aged mice
Am J Physiol Regulatory Integrative Comp Physiol, January 1, 2008; 294(1): R179 - R184.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
J. Wang, H. Jiao, T. L. Stewart, M. V. H. Lyons, H. A. Shankowsky, P. G. Scott, and E. E. Tredget
Accelerated wound healing in leukocyte-specific, protein 1-deficient mouse is associated with increased infiltration of leukocytes and fibrocytes
J. Leukoc. Biol., December 1, 2007; 82(6): 1554 - 1563.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
K. E. Inouye, H. Shi, J. K. Howard, C. H. Daly, G. M. Lord, B. J. Rollins, and J. S. Flier
Absence of CC Chemokine Ligand 2 Does Not Limit Obesity-Associated Infiltration of Macrophages Into Adipose Tissue
Diabetes, September 1, 2007; 56(9): 2242 - 2250.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. Gutierrez-Fernandez, M. Inada, M. Balbin, A. Fueyo, A. S. Pitiot, A. Astudillo, K. Hirose, M. Hirata, S. D. Shapiro, A. Noel, et al.
Increased inflammation delays wound healing in mice deficient in collagenase-2 (MMP-8)
FASEB J, August 1, 2007; 21(10): 2580 - 2591.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Crit. Care Med.Home page
C. P. Baran, J. M. Opalek, S. McMaken, C. A. Newland, J. M. O'Brien Jr., M. G. Hunter, B. D. Bringardner, M. M. Monick, D. R. Brigstock, P. C. Stromberg, et al.
Important Roles for Macrophage Colony-stimulating Factor, CC Chemokine Ligand 2, and Mononuclear Phagocytes in the Pathogenesis of Pulmonary Fibrosis
Am. J. Respir. Crit. Care Med., July 1, 2007; 176(1): 78 - 89.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
T.A. Silva, G.P. Garlet, S.Y. Fukada, J.S. Silva, and F.Q. Cunha
Chemokines in Oral Inflammatory Diseases: Apical Periodontitis and Periodontal Disease
J. Dent. Res., April 1, 2007; 86(4): 306 - 319.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. A. Eming, S. Werner, P. Bugnon, C. Wickenhauser, L. Siewe, O. Utermohlen, J. M. Davidson, T. Krieg, and A. Roers
Accelerated Wound Closure in Mice Deficient for Interleukin-10
Am. J. Pathol., January 1, 2007; 170(1): 188 - 202.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
C. Lamagna, M. Aurrand-Lions, and B. A. Imhof
Dual role of macrophages in tumor growth and angiogenesis
J. Leukoc. Biol., October 1, 2006; 80(4): 705 - 713.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
J. G. Madson, D. T. Lynch, K. L. Tinkum, S. K. Putta, and L. A. Hansen
Erbb2 Regulates Inflammation and Proliferation in the Skin after Ultraviolet Irradiation
Am. J. Pathol., October 1, 2006; 169(4): 1402 - 1414.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
F. Hildebrand, W. J. Hubbard, M. A. Choudhry, M. Frink, H.-C. Pape, S. L. Kunkel, and I. H. Chaudry
Kupffer Cells and Their Mediators: The Culprits in Producing Distant Organ Damage after Trauma-Hemorrhage
Am. J. Pathol., September 1, 2006; 169(3): 784 - 794.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
L. A. Beck, B. Tancowny, M. E. Brummet, S. Y. Asaki, S. L. Curry, M. B. Penno, M. Foster, A. Bahl, and C. Stellato
Functional Analysis of the Chemokine Receptor CCR3 on Airway Epithelial Cells.
J. Immunol., September 1, 2006; 177(5): 3344 - 3354.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
S. M. Stamatovic, R. F. Keep, M. Mostarica-Stojkovic, and A. V. Andjelkovic
CCL2 Regulates Angiogenesis via Activation of Ets-1 Transcription Factor
J. Immunol., August 15, 2006; 177(4): 2651 - 2661.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
H. Sell, D. Dietze-Schroeder, U. Kaiser, and J. Eckel
Monocyte Chemotactic Protein-1 Is a Potential Player in the Negative Cross-Talk between Adipose Tissue and Skeletal Muscle
Endocrinology, May 1, 2006; 147(5): 2458 - 2467.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
Y. Zhao, T. Shimizu, J. Nishihira, Y. Koyama, T. Kushibiki, A. Honda, H. Watanabe, R. Abe, Y. Tabata, and H. Shimizu
Tissue Regeneration Using Macrophage Migration Inhibitory Factor-Impregnated Gelatin Microbeads in Cutaneous Wounds
Am. J. Pathol., December 1, 2005; 167(6): 1519 - 1529.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
S. Saika, K. Ikeda, O. Yamanaka, T. Miyamoto, Y. Ohnishi, M. Sato, Y. Muragaki, A. Ooshima, Y. Nakajima, W. W.-Y. Kao, et al.
Expression of Smad7 in Mouse Eyes Accelerates Healing of Corneal Tissue after Exposure to Alkali
Am. J. Pathol., May 1, 2005; 166(5): 1405 - 1418.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
O. Dewald, P. Zymek, K. Winkelmann, A. Koerting, G. Ren, T. Abou-Khamis, L. H. Michael, B. J. Rollins, M. L. Entman, and N. G. Frangogiannis
CCL2/Monocyte Chemoattractant Protein-1 Regulates Inflammatory Responses Critical to Healing Myocardial Infarcts
Circ. Res., April 29, 2005; 96(8): 881 - 889.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
J. C. I. Singh, S. M. Cruickshank, D. J. Newton, L. Wakenshaw, A. Graham, J. Lan, J. P. A. Lodge, P. J. Felsburg, and S. R. Carding
Toll-like receptor-mediated responses of primary intestinal epithelial cells during the development of colitis
Am J Physiol Gastrointest Liver Physiol, March 1, 2005; 288(3): G514 - G524.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
T. R. Kyriakides, M. J. Foster, G. E. Keeney, A. Tsai, C. M. Giachelli, I. Clark-Lewis, B. J. Rollins, and P. Bornstein
The CC Chemokine Ligand, CCL2/MCP1, Participates in Macrophage Fusion and Foreign Body Giant Cell Formation
Am. J. Pathol., December 1, 2004; 165(6): 2157 - 2166.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
G. Lakos, S. Takagawa, S.-J. Chen, A. M. Ferreira, G. Han, K. Masuda, X.-J. Wang, L. A. DiPietro, and J. Varga
Targeted Disruption of TGF-{beta}/Smad3 Signaling Modulates Skin Fibrosis in a Mouse Model of Scleroderma
Am. J. Pathol., July 1, 2004; 165(1): 203 - 217.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
G. L. Warren, L. O'Farrell, M. Summan, T. Hulderman, D. Mishra, M. I. Luster, W. A. Kuziel, and P. P. Simeonova
Role of CC chemokines in skeletal muscle functional restoration after injury
Am J Physiol Cell Physiol, May 1, 2004; 286(5): C1031 - C1036.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
R. Mori, T. Kondo, T. Nishie, T. Ohshima, and M. Asano
Impairment of Skin Wound Healing in {beta}-1,4-Galactosyltransferase-Deficient Mice with Reduced Leukocyte Recruitment
Am. J. Pathol., April 1, 2004; 164(4): 1303 - 1314.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
H. Amano, K. Morimoto, M. Senba, H. Wang, Y. Ishida, A. Kumatori, H. Yoshimine, K. Oishi, N. Mukaida, and T. Nagatake
Essential Contribution of Monocyte Chemoattractant Protein-1/C-C Chemokine Ligand-2 to Resolution and Repair Processes in Acute Bacterial Pneumonia
J. Immunol., January 1, 2004; 172(1): 398 - 409.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. W. M. Minto, L.-P. Erwig, and A. J. Rees
Heterogeneity of Macrophage Activation in Anti-Thy-1.1 Nephritis
Am. J. Pathol., November 1, 2003; 163(5): 2033 - 2041.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
M. L. Lindsey, J. Yoshioka, C. MacGillivray, S. Muangman, J. Gannon, A. Verghese, M. Aikawa, P. Libby, S. M. Krane, and R. T. Lee
Effect of a Cleavage-Resistant Collagen Mutation on Left Ventricular Remodeling
Circ. Res., August 8, 2003; 93(3): 238 - 245.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
S. WERNER and R. GROSE
Regulation of Wound Healing by Growth Factors and Cytokines
Physiol Rev, July 1, 2003; 83(3): 835 - 870.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Sartipy and D. J. Loskutoff
Monocyte chemoattractant protein 1 in obesity and insulin resistance
PNAS, June 10, 2003; 100(12): 7265 - 7270.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
H. Shimizu, S. Maruyama, Y. Yuzawa, T. Kato, Y. Miki, S. Suzuki, W. Sato, Y. Morita, H. Maruyama, K. Egashira, et al.
Anti-Monocyte Chemoattractant Protein-1 Gene Therapy Attenuates Renal Injury Induced by Protein-Overload Proteinuria
J. Am. Soc. Nephrol., June 1, 2003; 14(6): 1496 - 1505.
[Abstract] [Full Text] [PDF]

Home page
 J. Leukoc. Biol.Home page
S. Yoshida, A. Yoshida, T. Ishibashi, S. G. Elner, and V. M. Elner
Role of MCP-1 and MIP-1{alpha} in retinal neovascularization during postischemic inflammation in a mouse model of retinal neovascularization
J. Leukoc. Biol., January 1, 2003; 73(1): 137 - 144.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
A. Agah, T. R. Kyriakides, J. Lawler, and P. Bornstein
The Lack of Thrombospondin-1 (TSP1) Dictates the Course of Wound Healing in Double-TSP1/TSP2-Null Mice
Am. J. Pathol., September 1, 2002; 161(3): 831 - 839.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2001 by the American Society for Investigative Pathology.