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(American Journal of Pathology. 2001;159:483-491.)
© 2001 American Society for Investigative Pathology

Technical Advances

The Generation and Characterization of a Cell Line Derived from a Sporadic Renal Angiomyolipoma

Use of Telomerase to Obtain Stable Populations of Cells from Benign Neoplasms

Jack L. Arbiser*, Raymond Yeung{dagger}, Sharon W. Weiss{ddagger}, Zoya K. Arbiser{ddagger}, Mahul B. Amin{ddagger}, Cynthia Cohen{ddagger}, David Frank§, Sudipta Mahajan§, G. Scott Herron, Jiwei Yang, Hiroki Onda§, H. B. Zhang§, Xianhe Bai*, Erik Uhlmann||, Allison Loehr||, Hope Northrup**, Paul Au**, Ian Davis{dagger}{dagger}, David E. Fisher{dagger}{dagger} and David H. Gutmann||

From the Departments of Dermatology

* and Pathology,

{ddagger} Emory University School of Medicine and the Winship Cancer Institute, Atlanta, Georgia; the Department of Internal Medicine,

{dagger} University of Washington School of Medicine, Seattle, Washington; the Departments of Adult Oncology

§ and Pediatric Oncology,

{dagger}{dagger} Dana Farber Cancer Institute and the Harvard Medical School, Boston, Massachusetts; the Department of Dermatology,

Stanford University School of Medicine, Stanford, California; the Department of Neurology,

|| Washington University School of Medicine, St. Louis, Missouri; and the Department of Pediatrics/Medical Genetics,

** University of Texas–Houston Division of Pediatric Hematology/Oncology, Houston, Texas

Angiomyolipomas are benign tumors of the kidney derived from putative perivascular epithelioid cells, that may undergo differentiation into cells with features of melanocytes, smooth muscle, and fat. To gain further insight into angiomyolipomas, we have generated the first human angiomyolipoma cell line by sequential introduction of SV40 large T antigen and human telomerase into human angiomyolipoma cells. These cells show phenotypic characteristics of angiomyolipomas, namely differentiation markers of smooth muscle (smooth muscle actin), adipose tissue (peroxisome proliferator-activator receptor {gamma}, PPAR{gamma}), and melanocytes (microophthalmia, MITF), thus demonstrating that a single cell type can exhibit all of these phenotypes. These cells should serve as a valuable tool to elucidate signal transduction pathways underlying renal angiomyolipomas.




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